Transcript: Human XM_017001265.1

PREDICTED: Homo sapiens calpain 8 (CAPN8), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CAPN8 (388743)
Length:
1868
CDS:
13..1641

Additional Resources:

NCBI RefSeq record:
XM_017001265.1
NBCI Gene record:
CAPN8 (388743)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017001265.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000352323 GGAGACCCTCTTCAAACTATT pLKO_005 1545 CDS 100% 13.200 18.480 N CAPN8 n/a
2 TRCN0000352324 TCTATTGGGAAACTGATTATA pLKO_005 1367 CDS 100% 15.000 12.000 N CAPN8 n/a
3 TRCN0000352440 ATGCGGGAATCTTTCACTTTC pLKO_005 67 CDS 100% 10.800 7.560 N CAPN8 n/a
4 TRCN0000377606 CCACTGCTTGCTTCTTGTCAC pLKO_005 1676 3UTR 100% 4.050 2.835 N CAPN8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017001265.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10096 pDONR223 100% 77% 72.6% None (many diffs) n/a
2 ccsbBroad304_10096 pLX_304 0% 77% 72.6% V5 (many diffs) n/a
3 TRCN0000478113 CACCGTATCTTCTGGCCACGATAA pLX_317 20.5% 77% 72.6% V5 (many diffs) n/a
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