Construct: ORF TRCN0000478113

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016764.1_s317c1
Derived from:
ccsbBroadEn_10096
DNA Barcode:
CACCGTATCTTCTGGCCACGATAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CAPN8 (388743)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478113
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 388743 CAPN8 calpain 8 NM_001143962.2 99.9% 99.7% 734C>A;1775C>T
2 human 388743 CAPN8 calpain 8 XM_017001265.1 77% 72.6% (many diffs)
3 human 388743 CAPN8 calpain 8 XM_017001266.1 66.2% 57.1% (many diffs)
4 human 388743 CAPN8 calpain 8 XM_017001267.2 61% 54.4% (many diffs)
5 human 388743 CAPN8 calpain 8 XM_017001268.1 53.9% 53.6% (many diffs)
6 mouse 170725 Capn8 calpain 8 NM_001145806.1 46.3% 46.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2178
ORF length:
2109
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcagcccag gcagctggtg tatctaggca gcgggcagcc actcaaggtc
121ttggctccaa ccaaaacgct ttgaagtact tgggccagga tttcaagacc ctgaggcaac
181agtgcttgga ctcaggggtc ctatttaagg accctgagtt cccagcatgt ccatcagctt
241tgggctacaa ggatcttgga ccaggctctc cgcaaactca aggcatcatc tggaagcggc
301ccacggagtt gtgtcccagc cctcagttta tcgttggtgg agccacgcgc acagacattt
361gtcagggtgg tctaggtgac tgctggcttc tggctgccat tgcctccctg accctgaatg
421aagagctgct ttaccgggtg gtccccaggg accaggactt ccaggagaac tatgcgggaa
481tctttcactt tcagttctgg cagtacggag agtgggtgga ggtggtcatt gacgacaggc
541tgcccaccaa gaatggacag ctgctcttcc tacactcgga acaaggcaat gaattctgga
601gtgccctgct ggagaaagcc tatgccaagc ttaatggttg ttatgaggct ctcgctggag
661gttccacagt ggaggggttt gaggatttca caggtggcat ctctgagttt tatgacctga
721agaaaccacc agccaatcta tatcagatca tccggaaggc cctctgtgcg gggtctctgc
781tgggctgctc cattgatgtc tacagtgcag ccgaagccga agccatcacc agccagaagc
841tggttaagag tcatgcgtac tctgtcactg gagtcgaaga ggtgaatttc cagggccatc
901cagagaagct gatcagactc aggaatccat ggggtgaagt ggagtggtcg ggagcctgga
961gcgatgatgc accagagtgg aatcacatag acccccggcg gaaggaagaa ctggacaaga
1021aagttgagga tggagaattc tggatgtcac tttcagattt cgtgaggcag ttctctcggt
1081tggagatctg caacctgtcc ccggactctc tgagtagcga ggaggtgcac aaatggaacc
1141tggtcctgtt caacggccac tggacccggg gctccacagc tgggggctgc cagaactacc
1201cagccacgta ctggaccaat ccccagttca aaatccgttt ggatgaagtg gatgaggacc
1261aggaggagag catcggtgaa ccctgctgta cagtgctgct gggcctgatg cagaaaaatc
1321gcaggtggcg gaagcggata ggacaaggca tgcttagcat cggctatgcc gtctaccagg
1381ttcccaagga gctggagagt cacacggacg cacacttggg ccgggatttc ttcctggcct
1441accagccctc agcccgcacc agcacctacg tcaacctgcg ggaggtctct ggccgggccc
1501ggctgccccc tggggagtac ctggtggtgc catccacatt tgaacccttc aaagacggcg
1561agttctgctt gagagtgttc tcagagaaga aggcccaggc cctagaaatt ggggatgtgg
1621tagctggaaa cccatatgag ccacatccca gtgaggtgga tcaggaagat gaccagttca
1681ggaggctgtt tgagaagttg gcagggaagg attctgagat tactgccaat gcactcaaga
1741tacttttgaa TGAGGCGTTT TCCAAGAGAA CAGACATAAA ATTCGATGGA TTCAACATCA
1801ACACTTGCAG GGAAATGATC AGTCTGTTGG ATAGCAATGG AATGGGCACT TTGGGGGCGG
1861TGGAATTCAA GACGCTCTGG CTGAAGATTC AGAAGTATCT GGAGATCTAT TGGGAAACTG
1921ATTATAACCA CTCGGGCACC ATCGATGCCC ACGAGATGAG GACAGCCCTC AGGAAGGCAG
1981GTTTCACCCT CAACAGCCAG GTGCAGCAGA CCATTGCCCT GCGGTATGCG TGCAGCAAGC
2041TTGGCATCAA CTTTGACAGC TTCGTGGCTT GTATGATCCG CCTGGAGACC CTCTTCAAAC
2101TATTCAGCCT TCTGGACGAA GACAAGGATG GCATGGTTCA GCTCTCTCTG GCCGAGTGGC
2161TGTGCTGCGT GTTGGTCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
2221TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
2281GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACACCGTA TCTTCTGGCC
2341ACGATAAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)