Construct: ORF TRCN0000478113
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016764.1_s317c1
- Derived from:
- ccsbBroadEn_10096
- DNA Barcode:
- CACCGTATCTTCTGGCCACGATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAPN8 (388743)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478113
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 388743 | CAPN8 | calpain 8 | NM_001143962.2 | 99.9% | 99.7% | 734C>A;1775C>T |
| 2 | human | 388743 | CAPN8 | calpain 8 | XM_017001265.1 | 77% | 72.6% | (many diffs) |
| 3 | human | 388743 | CAPN8 | calpain 8 | XM_017001266.1 | 66.2% | 57.1% | (many diffs) |
| 4 | human | 388743 | CAPN8 | calpain 8 | XM_017001267.2 | 61% | 54.4% | (many diffs) |
| 5 | human | 388743 | CAPN8 | calpain 8 | XM_017001268.1 | 53.9% | 53.6% | (many diffs) |
| 6 | mouse | 170725 | Capn8 | calpain 8 | NM_001145806.1 | 46.3% | 46.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2178
- ORF length:
- 2109
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagcccag gcagctggtg tatctaggca gcgggcagcc actcaaggtc 121 ttggctccaa ccaaaacgct ttgaagtact tgggccagga tttcaagacc ctgaggcaac 181 agtgcttgga ctcaggggtc ctatttaagg accctgagtt cccagcatgt ccatcagctt 241 tgggctacaa ggatcttgga ccaggctctc cgcaaactca aggcatcatc tggaagcggc 301 ccacggagtt gtgtcccagc cctcagttta tcgttggtgg agccacgcgc acagacattt 361 gtcagggtgg tctaggtgac tgctggcttc tggctgccat tgcctccctg accctgaatg 421 aagagctgct ttaccgggtg gtccccaggg accaggactt ccaggagaac tatgcgggaa 481 tctttcactt tcagttctgg cagtacggag agtgggtgga ggtggtcatt gacgacaggc 541 tgcccaccaa gaatggacag ctgctcttcc tacactcgga acaaggcaat gaattctgga 601 gtgccctgct ggagaaagcc tatgccaagc ttaatggttg ttatgaggct ctcgctggag 661 gttccacagt ggaggggttt gaggatttca caggtggcat ctctgagttt tatgacctga 721 agaaaccacc agccaatcta tatcagatca tccggaaggc cctctgtgcg gggtctctgc 781 tgggctgctc cattgatgtc tacagtgcag ccgaagccga agccatcacc agccagaagc 841 tggttaagag tcatgcgtac tctgtcactg gagtcgaaga ggtgaatttc cagggccatc 901 cagagaagct gatcagactc aggaatccat ggggtgaagt ggagtggtcg ggagcctgga 961 gcgatgatgc accagagtgg aatcacatag acccccggcg gaaggaagaa ctggacaaga 1021 aagttgagga tggagaattc tggatgtcac tttcagattt cgtgaggcag ttctctcggt 1081 tggagatctg caacctgtcc ccggactctc tgagtagcga ggaggtgcac aaatggaacc 1141 tggtcctgtt caacggccac tggacccggg gctccacagc tgggggctgc cagaactacc 1201 cagccacgta ctggaccaat ccccagttca aaatccgttt ggatgaagtg gatgaggacc 1261 aggaggagag catcggtgaa ccctgctgta cagtgctgct gggcctgatg cagaaaaatc 1321 gcaggtggcg gaagcggata ggacaaggca tgcttagcat cggctatgcc gtctaccagg 1381 ttcccaagga gctggagagt cacacggacg cacacttggg ccgggatttc ttcctggcct 1441 accagccctc agcccgcacc agcacctacg tcaacctgcg ggaggtctct ggccgggccc 1501 ggctgccccc tggggagtac ctggtggtgc catccacatt tgaacccttc aaagacggcg 1561 agttctgctt gagagtgttc tcagagaaga aggcccaggc cctagaaatt ggggatgtgg 1621 tagctggaaa cccatatgag ccacatccca gtgaggtgga tcaggaagat gaccagttca 1681 ggaggctgtt tgagaagttg gcagggaagg attctgagat tactgccaat gcactcaaga 1741 tacttttgaa TGAGGCGTTT TCCAAGAGAA CAGACATAAA ATTCGATGGA TTCAACATCA 1801 ACACTTGCAG GGAAATGATC AGTCTGTTGG ATAGCAATGG AATGGGCACT TTGGGGGCGG 1861 TGGAATTCAA GACGCTCTGG CTGAAGATTC AGAAGTATCT GGAGATCTAT TGGGAAACTG 1921 ATTATAACCA CTCGGGCACC ATCGATGCCC ACGAGATGAG GACAGCCCTC AGGAAGGCAG 1981 GTTTCACCCT CAACAGCCAG GTGCAGCAGA CCATTGCCCT GCGGTATGCG TGCAGCAAGC 2041 TTGGCATCAA CTTTGACAGC TTCGTGGCTT GTATGATCCG CCTGGAGACC CTCTTCAAAC 2101 TATTCAGCCT TCTGGACGAA GACAAGGATG GCATGGTTCA GCTCTCTCTG GCCGAGTGGC 2161 TGTGCTGCGT GTTGGTCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2221 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2281 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACACCGTA TCTTCTGGCC 2341 ACGATAAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt