Transcript: Human XM_017005007.1

PREDICTED: Homo sapiens mitogen-activated protein kinase kinase kinase 19 (MAP3K19), transcript variant X12, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MAP3K19 (80122)
Length:
4688
CDS:
121..3381

Additional Resources:

NCBI RefSeq record:
XM_017005007.1
NBCI Gene record:
MAP3K19 (80122)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146020 GTAAGGACACCAGATCGCGT pXPR_003 GGG 702 22% 7 1.0492 MAP3K19 MAP3K19 76693
2 BRDN0001148579 ATGGAGCCGAACATATCTCA pXPR_003 CGG 2111 65% 7 1.0316 MAP3K19 MAP3K19 76694
3 BRDN0001148706 GGGGTCATCAAAGACTACAC pXPR_003 TGG 1387 43% 7 0.7132 MAP3K19 MAP3K19 76695
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017005007.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196258 GCAGACGTATCACCAATAAAT pLKO.1 2252 CDS 100% 15.000 21.000 N MAP3K19 n/a
2 TRCN0000355773 TGGCTCAATCTCTAGTATTAT pLKO_005 3574 3UTR 100% 15.000 21.000 N MAP3K19 n/a
3 TRCN0000002392 TCTGGCTATGGACGGAAATCA pLKO.1 3863 3UTR 100% 5.625 4.500 N MAP3K19 n/a
4 TRCN0000363113 ACACGTATTACCGTGAGATAT pLKO_005 2216 CDS 100% 13.200 9.240 N MAP3K19 n/a
5 TRCN0000355772 CCGACAGAGCCAAGACTAAAT pLKO_005 2071 CDS 100% 13.200 9.240 N MAP3K19 n/a
6 TRCN0000002394 GATGGTGTTCTGTAAATATAC pLKO.1 3619 3UTR 100% 13.200 9.240 N MAP3K19 n/a
7 TRCN0000002391 AGCATTGGTTGTACTGTGTTT pLKO.1 3893 3UTR 100% 4.950 3.465 N MAP3K19 n/a
8 TRCN0000002393 GAGCATTGGTTGTACTGTGTT pLKO.1 3892 3UTR 100% 4.950 3.465 N MAP3K19 n/a
9 TRCN0000194794 CCAACTGATTTGATGACAGTT pLKO.1 244 CDS 100% 0.495 0.347 N MAP3K19 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017005007.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.