Transcript: Human XM_017014193.2

PREDICTED: Homo sapiens semaphorin 4D (SEMA4D), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SEMA4D (10507)
Length:
6325
CDS:
2268..4856

Additional Resources:

NCBI RefSeq record:
XM_017014193.2
NBCI Gene record:
SEMA4D (10507)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017014193.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000058147 GTACGGTCTTATGGGCAGAAA pLKO.1 4067 CDS 100% 4.950 6.930 N SEMA4D n/a
2 TRCN0000058146 CCTGAACTTAACATCCTTTAA pLKO.1 2678 CDS 100% 13.200 9.240 N SEMA4D n/a
3 TRCN0000058144 CGAACCAAAGATCGTCATCAA pLKO.1 4391 CDS 100% 4.950 3.465 N SEMA4D n/a
4 TRCN0000058143 GCCTGTGACTTGGTTTCTCTT pLKO.1 5019 3UTR 100% 4.950 3.465 N SEMA4D n/a
5 TRCN0000058145 GCCTGTGTTCTATGCACTCTT pLKO.1 3185 CDS 100% 4.950 3.465 N SEMA4D n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017014193.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07622 pDONR223 100% 99.9% 99.8% None 1775G>A n/a
2 ccsbBroad304_07622 pLX_304 0% 99.9% 99.8% V5 1775G>A n/a
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