Construct: ORF ccsbBroad304_07622
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013730.1_s304c1, BRDN0000410821
- Derived from:
- ccsbBroadEn_07622
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA4D (10507)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07622
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371194.2 | 99.9% | 99.8% | 1775G>A |
| 2 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371195.1 | 99.9% | 99.8% | 1775G>A |
| 3 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371196.1 | 99.9% | 99.8% | 1775G>A |
| 4 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371197.1 | 99.9% | 99.8% | 1775G>A |
| 5 | human | 10507 | SEMA4D | semaphorin 4D | NM_006378.4 | 99.9% | 99.8% | 1775G>A |
| 6 | human | 10507 | SEMA4D | semaphorin 4D | XM_011518123.2 | 99.9% | 99.8% | 1775G>A |
| 7 | human | 10507 | SEMA4D | semaphorin 4D | XM_011518124.2 | 99.9% | 99.8% | 1775G>A |
| 8 | human | 10507 | SEMA4D | semaphorin 4D | XM_011518125.1 | 99.9% | 99.8% | 1775G>A |
| 9 | human | 10507 | SEMA4D | semaphorin 4D | XM_011518127.2 | 99.9% | 99.8% | 1775G>A |
| 10 | human | 10507 | SEMA4D | semaphorin 4D | XM_011518128.2 | 99.9% | 99.8% | 1775G>A |
| 11 | human | 10507 | SEMA4D | semaphorin 4D | XM_011518129.1 | 99.9% | 99.8% | 1775G>A |
| 12 | human | 10507 | SEMA4D | semaphorin 4D | XM_011518130.2 | 99.9% | 99.8% | 1775G>A |
| 13 | human | 10507 | SEMA4D | semaphorin 4D | XM_011518131.2 | 99.9% | 99.8% | 1775G>A |
| 14 | human | 10507 | SEMA4D | semaphorin 4D | XM_011518133.2 | 99.9% | 99.8% | 1775G>A |
| 15 | human | 10507 | SEMA4D | semaphorin 4D | XM_011518134.2 | 99.9% | 99.8% | 1775G>A |
| 16 | human | 10507 | SEMA4D | semaphorin 4D | XM_017014193.2 | 99.9% | 99.8% | 1775G>A |
| 17 | human | 10507 | SEMA4D | semaphorin 4D | XM_017014194.1 | 99.9% | 99.8% | 1775G>A |
| 18 | human | 10507 | SEMA4D | semaphorin 4D | XM_017014195.1 | 99.9% | 99.8% | 1775G>A |
| 19 | human | 10507 | SEMA4D | semaphorin 4D | XM_017014198.1 | 99.9% | 99.8% | 1775G>A |
| 20 | human | 10507 | SEMA4D | semaphorin 4D | NM_001142287.2 | 76.2% | 63.9% | (many diffs) |
| 21 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371198.1 | 76.2% | 63.9% | (many diffs) |
| 22 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371199.1 | 76.2% | 63.9% | (many diffs) |
| 23 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371200.1 | 76.2% | 63.9% | (many diffs) |
| 24 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371201.1 | 76.2% | 63.9% | (many diffs) |
| 25 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371202.1 | 74.3% | 67.8% | (many diffs) |
| 26 | human | 10507 | SEMA4D | semaphorin 4D | XM_017014199.1 | 59.6% | 57.3% | (many diffs) |
| 27 | human | 10507 | SEMA4D | semaphorin 4D | XR_001746156.1 | 58.7% | (many diffs) | |
| 28 | human | 10507 | SEMA4D | semaphorin 4D | XR_001746153.1 | 58.4% | (many diffs) | |
| 29 | human | 10507 | SEMA4D | semaphorin 4D | NR_163886.1 | 51.6% | (many diffs) | |
| 30 | human | 10507 | SEMA4D | semaphorin 4D | NR_163887.1 | 51% | (many diffs) | |
| 31 | human | 10507 | SEMA4D | semaphorin 4D | NR_163884.1 | 50.8% | (many diffs) | |
| 32 | human | 10507 | SEMA4D | semaphorin 4D | NR_163885.1 | 50.2% | (many diffs) | |
| 33 | human | 10507 | SEMA4D | semaphorin 4D | NR_163888.1 | 49.9% | (many diffs) | |
| 34 | human | 10507 | SEMA4D | semaphorin 4D | NR_163889.1 | 49.7% | (many diffs) | |
| 35 | mouse | 20354 | Sema4d | sema domain, immunoglobulin... | NM_001281880.1 | 82.7% | 85.3% | (many diffs) |
| 36 | mouse | 20354 | Sema4d | sema domain, immunoglobulin... | NM_013660.4 | 82.7% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2652
- ORF length:
- 2586
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag gatgtgcacc cccattaggg ggctgctcat ggcccttgca gtgatgtttg 121 ggacagcgat ggcatttgca cccatacccc ggatcacctg ggagcacaga gaggtgcacc 181 tggtgcagtt tcatgagcca gacatctaca actactcagc cttgctgctg agcgaggaca 241 aggacacctt gtacataggt gcccgggagg cggtcttcgc tgtgaacgca ctcaacatct 301 ccgagaagca gcatgaggtg tattggaagg tctcagaaga caaaaaagca aaatgtgcag 361 aaaaggggaa atcaaaacag acagagtgcc tcaactacat ccgggtgctg cagccactca 421 gcgccacttc cctttacgtg tgtgggacca acgcattcca gccggcctgt gaccacctga 481 acttaacatc ctttaagttt ctggggaaaa atgaagatgg caaaggaaga tgtccctttg 541 acccagcaca cagctacaca tccgtcatgg ttgatggaga actttattcg gggacgtcgt 601 ataatttttt gggaagtgaa cccatcatct cccgaaattc ttcccacagt cctctgagga 661 cagaatatgc aatcccttgg ctgaacgagc ctagtttcgt gtttgctgac gtgatccgaa 721 aaagcccaga cagccccgac ggcgaggatg acagggtcta cttcttcttc acggaggtgt 781 ctgtggagta tgagtttgtg ttcagggtgc tgatcccacg gatagcaaga gtgtgcaagg 841 gggaccaggg cggcctgagg accttgcaga agaaatggac ctccttcctg aaagcccgac 901 tcatctgctc ccggccagac agcggcttgg tcttcaatgt gctgcgggat gtcttcgtgc 961 tcaggtcccc gggcctgaag gtgcctgtgt tctatgcact cttcacccca cagctgaaca 1021 acgtggggct gtcggcagtg tgcgcctaca acctgtccac agccgaggag gtcttctccc 1081 acgggaagta catgcagagc accacagtgg agcagtccca caccaagtgg gtgcgctata 1141 atggcccggt acccaagccg cggcctggag cgtgcatcga cagcgaggca cgggccgcca 1201 actacaccag ctccttgaat ttgccagaca agacgctgca gttcgttaaa gaccaccctt 1261 tgatggatga ctcggtaacc ccaatagaca acaggcccag gttaatcaag aaagatgtga 1321 actacaccca gatcgtggtg gaccggaccc aggccctgga tgggactgtc tatgatgtca 1381 tgtttgtcag cacagaccgg ggagctctgc acaaagccat cagcctcgag cacgctgttc 1441 acatcatcga ggagacccag ctcttccagg actttgagcc agtccagacc ctgctgctgt 1501 cttcaaagaa gggcaacagg tttgtctatg ctggctctaa ctcgggcgtg gtccaggccc 1561 cgctggcctt ctgtgggaag cacggcacct gcgaggactg tgtgctggcg cgggacccct 1621 actgcgcctg gagcccgccc acagcgacct gcgtggctct gcaccagacc gagagcccca 1681 gcaggggttt gattcaggag atgagcggcg atgcttctgt gtgcccggat aaaagtaaag 1741 gaagttaccg gcagcatttt ttcaagcacg gtggcacagc ggaactgaaa tgctcccaaa 1801 aatccaacct ggcccgggtc ttttggaagt tccagaatga cgtgttgaag gccgagagcc 1861 ccaagtacgg tcttatgggc agaaaaaact tgctcatctt caacttgtca gaaggagaca 1921 gtggggtgta ccagtgcctg tcagaggaga gggttaagaa caaaacggtc ttccaagtgg 1981 tcgccaagca cgtcctggaa gtgaaggtgg ttccaaagcc cgtagtggcc cccaccttgt 2041 cagttgttca gacagaaggt agtaggattg ccaccaaagt gttggtggca tccacccaag 2101 ggtcttctcc cccaacccca gccgtgcagg ccacctcctc cggggccatc acccttcctc 2161 ccaagcctgc gcccaccggc acatcctgcg aaccaaagat cgtcatcaac acggtccccc 2221 agctccactc ggagaaaacc atgtatctta agtccagcga caaccgcctc ctcatgtccc 2281 tcttcctctt cttctttgtt ctcttcctct gcctcttttt ctacaactgc tataagggat 2341 acctgcccag acagtgcttg aaattccgct cggccctact aattgggaag aagaagccca 2401 agtcagattt ctgtgaccgt gagcagagcc tgaaggagac gttagtagag ccagggagct 2461 tctcccagca gaatggggag caccccaagc cagccctgga caccggctat gagaccgagc 2521 aagacaccat caccagcaaa gtccccacgg atagggagga ctcacagagg atcgacgacc 2581 tttctgccag ggacaagccc tttgacgtca agtgtgagct gaagttcgct gactcagacg 2641 cagatggaga ctgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 2701 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 2761 caatcaacct ctggatta