Transcript: Human XM_017015049.1

PREDICTED: Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X14, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
WNK2 (65268)
Length:
8099
CDS:
233..6916

Additional Resources:

NCBI RefSeq record:
XM_017015049.1
NBCI Gene record:
WNK2 (65268)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001162416 AGGCCAACCTGTGTACCCAG pXPR_003 CGG 2737 41% 12 0.4716 WNK2 WNK2 75671
2 BRDN0001162262 GGAGCACTACGATGAGTCCG pXPR_003 TGG 1126 17% 5 0.2308 WNK2 WNK2 75673
3 BRDN0001162408 CAAGTCCATCCGTGACCGCG pXPR_003 TGG 1603 24% 8 0.1421 WNK2 WNK2 75672
4 BRDN0001148439 GAGGACACAGACGCCGACCG pXPR_003 TGG 3803 57% 17 0.0419 LOC124902214, WNK2 WNK2 75670
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017015049.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000002252 CCAAGATTCAGCGCCCTATAA pLKO.1 4261 CDS 100% 13.200 18.480 N WNK2 n/a
2 TRCN0000002255 CCGATGAAATTGCCACGTATA pLKO.1 3906 CDS 100% 10.800 15.120 N WNK2 n/a
3 TRCN0000199250 CGGTTCCTTCAAGACGGTCTA pLKO.1 841 CDS 100% 4.050 5.670 N WNK2 n/a
4 TRCN0000197192 GCTTACCATCTTGAACGTGTG pLKO.1 3796 CDS 100% 2.250 3.150 N WNK2 n/a
5 TRCN0000002254 CGAGACCTGAAATGTGACAAT pLKO.1 1196 CDS 100% 4.950 3.960 N WNK2 n/a
6 TRCN0000194923 CCTGAAATCAAGGAGATTATT pLKO.1 1502 CDS 100% 15.000 10.500 N WNK2 n/a
7 TRCN0000002253 GAGGAAAGGTACGAGATCAAA pLKO.1 1547 CDS 100% 5.625 3.938 N WNK2 n/a
8 TRCN0000195306 CCCAAGAGATGATTGAGTCTG pLKO.1 1764 CDS 100% 4.050 2.835 N WNK2 n/a
9 TRCN0000002256 GACGCTGAAGACATACCTGAA pLKO.1 1075 CDS 100% 4.050 2.835 N WNK2 n/a
10 TRCN0000199742 GCCAAGACTGTGGGCCGTTTC pLKO.1 5456 CDS 100% 0.000 0.000 N WNK2 n/a
11 TRCN0000360826 ACAATGGAGCCATAGAGTTTA pLKO_005 1707 CDS 100% 13.200 9.240 N Wnk2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017015049.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.