Transcript: Human XM_017015752.2

PREDICTED: Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
HECTD2 (143279)
Length:
4065
CDS:
407..1687

Additional Resources:

NCBI RefSeq record:
XM_017015752.2
NBCI Gene record:
HECTD2 (143279)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017015752.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000412949 GTCAGTACCCATTCGTTATTT pLKO_005 462 CDS 100% 15.000 21.000 N Hectd2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017015752.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491311 AGACCCACCAAAACAATGAAACTA pLX_317 9.4% 54.8% 54.8% V5 (not translated due to prior stop codon) 0_1ins1050 n/a
2 TRCN0000488652 GGGGAAACATCGGCAACATGCCAC pLX_317 14.4% 54.8% 54.8% V5 0_1ins1050;1278_1279insG n/a
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