Construct: ORF TRCN0000488652
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019930.1_s317c1
- DNA Barcode:
- GGGGAAACATCGGCAACATGCCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HECTD2 (143279)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488652
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 143279 | HECTD2 | HECT domain E3 ubiquitin pr... | NM_182765.5 | 99.9% | 99.8% | 2328_2329insG |
| 2 | human | 143279 | HECTD2 | HECT domain E3 ubiquitin pr... | NM_001284274.2 | 99.4% | 99.3% | 822_833del;2340_2341insG |
| 3 | human | 143279 | HECTD2 | HECT domain E3 ubiquitin pr... | NM_001348365.1 | 86.1% | 86.1% | 0_1ins321;2007_2008insG |
| 4 | human | 143279 | HECTD2 | HECT domain E3 ubiquitin pr... | XM_024447828.1 | 86.1% | 86.1% | 0_1ins321;2007_2008insG |
| 5 | human | 143279 | HECTD2 | HECT domain E3 ubiquitin pr... | XM_017015752.2 | 54.8% | 54.8% | 0_1ins1050;1278_1279insG |
| 6 | human | 143279 | HECTD2 | HECT domain E3 ubiquitin pr... | XM_017015751.1 | 51.5% | 51% | (many diffs) |
| 7 | human | 143279 | HECTD2 | HECT domain E3 ubiquitin pr... | NR_145526.1 | 46.9% | 1_154del;1676_1684delTATGCATAG;2492_4956delinsG | |
| 8 | human | 143279 | HECTD2 | HECT domain E3 ubiquitin pr... | NR_104291.2 | 46.2% | (many diffs) | |
| 9 | human | 143279 | HECTD2 | HECT domain E3 ubiquitin pr... | NM_173497.3 | 26.1% | 25.9% | (many diffs) |
| 10 | mouse | 226098 | Hectd2 | HECT domain E3 ubiquitin pr... | NM_172637.3 | 88.9% | 89.3% | (many diffs) |
| 11 | mouse | 226098 | Hectd2 | HECT domain E3 ubiquitin pr... | NM_001163471.1 | 88.8% | 89.2% | (many diffs) |
| 12 | mouse | 226098 | Hectd2 | HECT domain E3 ubiquitin pr... | XM_006526991.3 | 77.7% | 78.6% | (many diffs) |
| 13 | mouse | 226098 | Hectd2 | HECT domain E3 ubiquitin pr... | XM_017318159.1 | 60.9% | 60.8% | (many diffs) |
| 14 | mouse | 226098 | Hectd2 | HECT domain E3 ubiquitin pr... | XM_017318160.1 | 59.9% | 61.5% | (many diffs) |
| 15 | mouse | 226098 | Hectd2 | HECT domain E3 ubiquitin pr... | XM_017318161.1 | 58.2% | 56.8% | (many diffs) |
| 16 | mouse | 226098 | Hectd2 | HECT domain E3 ubiquitin pr... | XR_001782583.1 | 41.8% | (many diffs) | |
| 17 | mouse | 226098 | Hectd2 | HECT domain E3 ubiquitin pr... | XR_001782584.1 | 33.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2400
- ORF length:
- 2331
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gagtgaggcg gttcgggtac cctcgcccgc cactccgctg gtggtggcgg 121 cggccgcgcc tgaggagagg aaagggaagg agtcagagcg cgagaagctg ccgcccatcg 181 tatcggcggg cgccggcgcg accgcgggtt tggacagagg agccaaaggc caaatttcca 241 ctttcagcag ttttatttca gctgttagcc cgaagaaaga agctgctgaa aacagaagtt 301 cacctgcaca tcttgttttc cctaacatca agaatgtgag agaaccacca ccaatttgcc 361 ttgatgttag acaaaaacag cgtacatcta tggatgcatc atcatccgaa atgaaggccc 421 cagtccttcc agaacctatt cttcctatcc agcccaaaac tgtgaaagac tttcaggaag 481 atgtagaaaa agttaagtca tcaggagatt ggaaagcagt acatgatttt tatctaacaa 541 cgtttgattc tttcccagaa ttaaatgctg catttaagaa agatgccact gcctcattta 601 acaccattga agactctggg attaatgcta aatttgtgaa tgctgtgtat gataccttac 661 ttaatactcc tcaagacgtt cagaagacag tattaaaggg aatcattaac agcctgttac 721 gagaatggaa aggtccacga acaaaagatg atcttagagc atattttata cttttacaga 781 atcctcagtt taataacaca tctacgtatg tcatctatgc tcacttgcta cgacagatag 841 ctaccttagt ggaagctgac catcatttcc tagttcactg gtttaaaaaa ttgtcccaga 901 agaggttcaa acaattggta gagagattgc tgcaatttat ttctttacgc ctgtttcctg 961 caaagcctga agaatttcca cctataacaa agtgttcctg gtggattcca tcagccgcta 1021 aagtgttggc tttacttaat actgcaaaca atttagttca ccctcccctt attccttata 1081 ctgatttcta taattctaca ttggatcaca ttgatctcat ggaagaatat catacttggc 1141 aaaactttgg aaactctcac aggttttcct tctgtcagta cccattcgtt atttctgtag 1201 ctgcaaaaaa aatcattatt cagagagact cagagcaaca gatgataaac atcgcaaggc 1261 aaagtctggt ggataaagta tctcgaagac agagacctga tatgaatata ttatttctaa 1321 atatgaaagt aagacggaca catctggtta gcgattcact tgatgagcta acccggaaaa 1381 gagccgattt gaaaaagaag ttaaaagtta catttgtagg ggaagctggt ttggatatgg 1441 gtggtttgac taaagaatgg ttccttcttc taattcgcca aatttttcat ccagattatg 1501 gcatgtttac atatcacaag gattcacact gccattggtt tagcagcttt aaatgtgata 1561 actattctga attccgattg gttggaattc ttatgggact agctgtttat aacagcatca 1621 ccttggatat tcgtttccct ccctgctgtt acaagaaatt attgagccct cccatcattc 1681 ctagtgatca aaatatacca gtaggcatct gcaatgttac cgtggacgac ttatgtcaaa 1741 ttatgcctga gttggcccat ggattaagtg aactcttatc acatgaaggc aatgttgaag 1801 aagatttcta ttcaacattt caggtttttc aagaagaatt tggaataatc aagtcctata 1861 atttaaagcc cggtggtgat aaaatttcag ttaccaatca aaatagaaaa gaatatgtac 1921 agctttatac tgacttcctt ctgaacaaat ccatctataa gcagtttgct gcattttatt 1981 atggatttca tagtgtgtgt gcttcaaatg ccctaatgct gcttcgtcca gaagaggttg 2041 aaattttggt ctgtggaagt cctgacctGG ATATGCATGC TCTGCAGAGA AGTACTCAGT 2101 ATGATGGCTA TGCAAAAACG GACTTAACTA TAAAATACTT TTGGGATGTT GTGCTTGGAT 2161 TTCCTCTTGA TCTTCAAAAG AAGTTGCTAC ATTTTACTAC AGGGAGTGAC AGAGTACCTG 2221 TAGGAGGGAT GGCTGATTTG AACTTTAAAA TCTCAAAGAA TGAAACTTCT ACTAACTGCT 2281 TACCTGTGGC CCATACCTGC TTCAATCAAC TTTGCCTTCC CCCCTACAAG AGCAAAAAGG 2341 ATCTGAAACA GAAATTGATC ATTGGAATTT CAAATTCAGA AGGTTTTGGA CTTGAGGACC 2401 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2461 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2521 ATATATCTTG TGGAAAGGAC GAGGGGAAAC ATCGGCAACA TGCCACACGC GTTAAGTCga 2581 caatcaacct ctggattaca aaatttgtga aagatt