Transcript: Human XM_017018737.1

PREDICTED: Homo sapiens citron rho-interacting serine/threonine kinase (CIT), transcript variant X7, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CIT (11113)
Length:
8686
CDS:
84..6245

Additional Resources:

NCBI RefSeq record:
XM_017018737.1
NBCI Gene record:
CIT (11113)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146852 CCAGCTTGATGTGTCCTGTG pXPR_003 CGG 695 11% 7 0.8792 CIT CIT 76723
2 BRDN0001149187 AGACTCGGTGATCCGCTGAT pXPR_003 CGG 3364 55% 27 0.6957 CIT CIT 76721
3 BRDN0001149264 GCAGCTAAACCAGCTGACCG pXPR_003 AGG 2995 49% 25 -0.0647 CIT CIT 76722
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017018737.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000006315 GCGTCCTCATACCAGGATAAA pLKO.1 5820 CDS 100% 13.200 18.480 N CIT n/a
2 TRCN0000277814 GCGTCCTCATACCAGGATAAA pLKO_005 5820 CDS 100% 13.200 18.480 N CIT n/a
3 TRCN0000006316 CGGAAGTATTCCGACACCATA pLKO.1 315 CDS 100% 4.950 6.930 N CIT n/a
4 TRCN0000277746 CGGAAGTATTCCGACACCATA pLKO_005 315 CDS 100% 4.950 6.930 N CIT n/a
5 TRCN0000195505 CCATTTCCTCAGGAGCGATTT pLKO.1 5794 CDS 100% 10.800 7.560 N CIT n/a
6 TRCN0000006314 GCCAATAAACTTGCAGCAAAT pLKO.1 2568 CDS 100% 10.800 7.560 N CIT n/a
7 TRCN0000277745 GCCAATAAACTTGCAGCAAAT pLKO_005 2568 CDS 100% 10.800 7.560 N CIT n/a
8 TRCN0000006313 CCCTCCTATTAGAGTACGAAA pLKO.1 7667 3UTR 100% 4.950 3.465 N CIT n/a
9 TRCN0000277747 CCCTCCTATTAGAGTACGAAA pLKO_005 7667 3UTR 100% 4.950 3.465 N CIT n/a
10 TRCN0000197232 GCATCCAAATGTCTCGAATGT pLKO.1 4329 CDS 100% 4.950 3.465 N CIT n/a
11 TRCN0000006317 GCTGATCTACTGAAGACAGAA pLKO.1 3759 CDS 100% 4.950 3.465 N CIT n/a
12 TRCN0000277748 GCTGATCTACTGAAGACAGAA pLKO_005 3759 CDS 100% 4.950 3.465 N CIT n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017018737.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.