Transcript: Mouse XM_017318025.1

PREDICTED: Mus musculus RanBP-type and C3HC4-type zinc finger containing 1 (Rbck1), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Rbck1 (24105)
Length:
1845
CDS:
254..1345

Additional Resources:

NCBI RefSeq record:
XM_017318025.1
NBCI Gene record:
Rbck1 (24105)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017318025.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000041068 GCACATGAATTGCAGGGAGTA pLKO.1 1033 CDS 100% 4.050 3.240 N Rbck1 n/a
2 TRCN0000316811 GCACATGAATTGCAGGGAGTA pLKO_005 1033 CDS 100% 4.050 3.240 N Rbck1 n/a
3 TRCN0000041070 GCAGACGACAGAGATGCTAAA pLKO.1 1099 CDS 100% 10.800 7.560 N Rbck1 n/a
4 TRCN0000349204 GCAGACGACAGAGATGCTAAA pLKO_005 1099 CDS 100% 10.800 7.560 N Rbck1 n/a
5 TRCN0000041069 CCTACCAGATACCTGCTTCAT pLKO.1 641 CDS 100% 4.950 3.465 N Rbck1 n/a
6 TRCN0000316812 CCTACCAGATACCTGCTTCAT pLKO_005 641 CDS 100% 4.950 3.465 N Rbck1 n/a
7 TRCN0000041071 CCTCCCTTAAGGACATGGTAT pLKO.1 224 5UTR 100% 4.950 3.465 N Rbck1 n/a
8 TRCN0000041072 GCCGGGTCAATGGGATTCCAT pLKO.1 1296 CDS 100% 1.000 0.700 N Rbck1 n/a
9 TRCN0000316810 GCCGGGTCAATGGGATTCCAT pLKO_005 1296 CDS 100% 1.000 0.700 N Rbck1 n/a
10 TRCN0000338176 TCTTTGAGGATGATGTCAATG pLKO_005 954 CDS 100% 10.800 7.560 N RBCK1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017318025.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11529 pDONR223 100% 37% 17.4% None (many diffs) n/a
2 ccsbBroad304_11529 pLX_304 0% 37% 17.4% V5 (many diffs) n/a
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