Construct: ORF ccsbBroad304_11529
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012483.1_s304c1, BRDN0000402211
- Derived from:
- ccsbBroadEn_11529
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBCK1 (10616)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11529
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10616 | RBCK1 | RANBP2-type and C3HC4-type ... | XM_011529140.2 | 60.5% | 55% | (many diffs) |
| 2 | human | 10616 | RBCK1 | RANBP2-type and C3HC4-type ... | NM_006462.6 | 49.1% | 45.2% | 627_787del;852_1404del |
| 3 | human | 10616 | RBCK1 | RANBP2-type and C3HC4-type ... | XM_011529139.3 | 44.9% | 39.6% | (many diffs) |
| 4 | human | 10616 | RBCK1 | RANBP2-type and C3HC4-type ... | NM_031229.4 | 44.4% | 39.4% | (many diffs) |
| 5 | human | 10616 | RBCK1 | RANBP2-type and C3HC4-type ... | XM_005260645.2 | 43% | 38.2% | (many diffs) |
| 6 | human | 10616 | RBCK1 | RANBP2-type and C3HC4-type ... | XM_011529138.1 | 41% | 36.2% | (many diffs) |
| 7 | human | 10616 | RBCK1 | RANBP2-type and C3HC4-type ... | XM_011529137.2 | 39.7% | 35.1% | (many diffs) |
| 8 | human | 10616 | RBCK1 | RANBP2-type and C3HC4-type ... | NM_001323956.1 | 37.5% | 17% | 0_1ins223;468_1020del |
| 9 | human | 10616 | RBCK1 | RANBP2-type and C3HC4-type ... | NM_001323958.2 | 37.5% | 17% | 0_1ins223;468_1020del |
| 10 | human | 10616 | RBCK1 | RANBP2-type and C3HC4-type ... | XM_017027594.2 | 37.5% | 17% | 0_1ins223;468_1020del |
| 11 | human | 10616 | RBCK1 | RANBP2-type and C3HC4-type ... | XM_017027595.1 | 37.5% | 17% | 0_1ins223;468_1020del |
| 12 | human | 10616 | RBCK1 | RANBP2-type and C3HC4-type ... | XR_001754131.1 | 28.8% | 1_440del;480_624del;1276_2390del | |
| 13 | human | 10616 | RBCK1 | RANBP2-type and C3HC4-type ... | XR_937029.3 | 27.1% | 1_440del;481_799del;1450_2545del | |
| 14 | human | 10616 | RBCK1 | RANBP2-type and C3HC4-type ... | XR_001754130.2 | 26.8% | 1_440del;481_799del;1450_2568del | |
| 15 | human | 10616 | RBCK1 | RANBP2-type and C3HC4-type ... | NR_136659.2 | 18.7% | (many diffs) | |
| 16 | mouse | 24105 | Rbck1 | RanBP-type and C3HC4-type z... | XM_017318025.1 | 37% | 17.4% | (many diffs) |
| 17 | mouse | 24105 | Rbck1 | RanBP-type and C3HC4-type z... | XR_866272.2 | 24.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 757
- ORF length:
- 690
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttgggcatgg gcacagccac gccagatgga cgagaagacc aagaaaggct gtgggtgagc 121 gtggaggatg ctcagatgca caccgtcacc atctggctca cagtgcgccc tgatatgaca 181 gtggcgtctc tcaaggacat ggtttttctg gactatggct tcccaccagt cttgcagcag 241 tgggtgattg ggcagcggct ggcacgagac caggagaccc tgcactccca tggggtgcgg 301 cagaatgggg acagtgccta cctctatctg ctgtcagccc gcaacacctc cctcaaccct 361 caggagctgc agcgggagcg gcagctgcgg atgctggaag atctgggctt caaggacctc 421 acgctgcagc cgcggggccc tctggagcca ggccccccaa agcccggggt cccccaggaa 481 cccggacggg ggcagccaga tgcagtgcct gagcccccac cggtgggctg gcagtgcccc 541 gggtgcacct tcatcaacaa gcccacgcgg cctggctgtg agatgtgctg ccgggcgcgc 601 cccgaggcct accaggtccc cgcctcatac cagcccgacg aggaggagcg agcgcgcctg 661 gcgggcgagg aggaggcgct gcgtcagtac cagcagggag tgcctgcagg gcaccatccg 721 caacagccag gaggcggagg tctcctgccc cttcattgcc caactttctt gtacaaagtg 781 gttggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc 841 gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga tta