Transcript: Human XM_024453811.1

PREDICTED: Homo sapiens kalirin RhoGEF kinase (KALRN), transcript variant X15, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
KALRN (8997)
Length:
8275
CDS:
393..7466

Additional Resources:

NCBI RefSeq record:
XM_024453811.1
NBCI Gene record:
KALRN (8997)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145005 GCAGAATACGTACACCAATG pXPR_003 CGG 1783 25% 11 0.6641 KALRN KALRN 77639
2 BRDN0001146234 AAGCAGAAGAAAGTTCGCGA pXPR_003 TGG 5360 76% 35 0.5167 KALRN KALRN 77637
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024453811.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000234366 GCGGAAACTCGTGACGTATTT pLKO_005 620 CDS 100% 13.200 18.480 N KALRN n/a
2 TRCN0000048211 GCTCAGAATACGTACACCAAT pLKO.1 2157 CDS 100% 4.950 6.930 N KALRN n/a
3 TRCN0000048208 CCCGGAAGAAAGAATTTATTA pLKO.1 4207 CDS 100% 15.000 10.500 N KALRN n/a
4 TRCN0000234368 CTTCAGGACACACGAAATATG pLKO_005 4912 CDS 100% 13.200 9.240 N KALRN n/a
5 TRCN0000234369 TGATTCAAGAAAGGATCATTC pLKO_005 5104 CDS 100% 10.800 7.560 N KALRN n/a
6 TRCN0000001430 CAAAGATTACTATGCACTGAA pLKO.1 7256 CDS 100% 4.950 3.465 N KALRN n/a
7 TRCN0000048210 CCTGTCCAAAGGATCACCAAA pLKO.1 4611 CDS 100% 4.950 3.465 N KALRN n/a
8 TRCN0000048212 GCATATCATCTTTGGCAACAT pLKO.1 4349 CDS 100% 4.950 3.465 N KALRN n/a
9 TRCN0000048209 GCCATGAACAACATGACCTTT pLKO.1 2856 CDS 100% 4.950 3.465 N KALRN n/a
10 TRCN0000001429 GTGGAGTTAATGTGCCTTGTT pLKO.1 6543 CDS 100% 4.950 3.465 N KALRN n/a
11 TRCN0000234367 GGACCTGGAGCTGGATATTAT pLKO_005 4109 CDS 100% 15.000 9.000 N KALRN n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024453811.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.