Transcript: Human XR_001746389.1

PREDICTED: Homo sapiens GTPase activating Rap/RanGAP domain like 3 (GARNL3), transcript variant X13, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GARNL3 (84253)
Length:
3475
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001746389.1
NBCI Gene record:
GARNL3 (84253)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001746389.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000048308 CGCTCCCACTTTACACATATT pLKO.1 1223 3UTR 100% 13.200 18.480 N GARNL3 n/a
2 TRCN0000048309 GCAGCTATTGATGTGTACGAA pLKO.1 2251 3UTR 100% 3.000 4.200 N GARNL3 n/a
3 TRCN0000048312 CCTTGGAAAGTGCTTCTACTT pLKO.1 3029 3UTR 100% 4.950 3.465 N GARNL3 n/a
4 TRCN0000048310 GCCATATTCCAAAGAGAACAA pLKO.1 1102 3UTR 100% 4.950 3.465 N GARNL3 n/a
5 TRCN0000106232 CCCGTGTTTGACAGAACTCTA pLKO.1 1705 3UTR 100% 4.950 3.465 N Garnl3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001746389.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15192 pDONR223 82.6% 79.7% None (many diffs) n/a
2 ccsbBroad304_15192 pLX_304 0% 79.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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