Construct: ORF ccsbBroad304_15192

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014038.1_s304c1, BRDN0000462243
Derived from:
ccsbBroadEn_15192
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
GARNL3 (84253)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15192
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84253 GARNL3 GTPase activating Rap/RanGA... NM_001286779.1 99.4% 35.2% (many diffs)
2 human 84253 GARNL3 GTPase activating Rap/RanGA... NM_032293.5 98% 35.2% (many diffs)
3 human 84253 GARNL3 GTPase activating Rap/RanGA... XM_017015202.1 96.4% 34.2% (many diffs)
4 human 84253 GARNL3 GTPase activating Rap/RanGA... XM_005252268.2 95.6% 33.7% (many diffs)
5 human 84253 GARNL3 GTPase activating Rap/RanGA... XM_011519089.3 95.6% 33.7% (many diffs)
6 human 84253 GARNL3 GTPase activating Rap/RanGA... XM_024447693.1 95.6% 33.7% (many diffs)
7 human 84253 GARNL3 GTPase activating Rap/RanGA... XM_011519086.3 93.3% 32.9% (many diffs)
8 human 84253 GARNL3 GTPase activating Rap/RanGA... XM_011519087.2 93.3% 32.9% (many diffs)
9 human 84253 GARNL3 GTPase activating Rap/RanGA... XM_005252267.3 92.1% 32.5% (many diffs)
10 human 84253 GARNL3 GTPase activating Rap/RanGA... XM_011519090.2 84.7% 20.8% (many diffs)
11 human 84253 GARNL3 GTPase activating Rap/RanGA... XM_024447694.1 84.7% 20.8% (many diffs)
12 human 84253 GARNL3 GTPase activating Rap/RanGA... XM_024447695.1 84.7% 20.8% (many diffs)
13 human 84253 GARNL3 GTPase activating Rap/RanGA... XM_024447696.1 84.7% 20.8% (many diffs)
14 human 84253 GARNL3 GTPase activating Rap/RanGA... XR_001746389.1 79.7% (many diffs)
15 human 84253 GARNL3 GTPase activating Rap/RanGA... NR_104591.1 75.7% (many diffs)
16 human 84253 GARNL3 GTPase activating Rap/RanGA... XM_024447697.1 55.9% 28.5% (many diffs)
17 mouse 99326 Garnl3 GTPase activating RANGAP do... NM_178888.4 88% 34.2% (many diffs)
18 mouse 99326 Garnl3 GTPase activating RANGAP do... XM_006498486.3 86.1% 33.5% (many diffs)
19 mouse 99326 Garnl3 GTPase activating RANGAP do... XM_006498483.3 84.6% 32.7% (many diffs)
20 mouse 99326 Garnl3 GTPase activating RANGAP do... XM_006498484.3 84.6% 32.7% (many diffs)
21 mouse 99326 Garnl3 GTPase activating RANGAP do... XM_006498485.3 84.6% 32.7% (many diffs)
22 mouse 99326 Garnl3 GTPase activating RANGAP do... XM_006498482.3 81.8% 31.6% (many diffs)
23 mouse 99326 Garnl3 GTPase activating RANGAP do... XM_006498481.3 81.5% 31.5% (many diffs)
24 mouse 99326 Garnl3 GTPase activating RANGAP do... XM_006498487.3 74.8% 20% (many diffs)
25 mouse 99326 Garnl3 GTPase activating RANGAP do... XM_006498488.3 74.8% 20% (many diffs)
26 mouse 99326 Garnl3 GTPase activating RANGAP do... XM_011239223.2 74.8% 20% (many diffs)
27 mouse 99326 Garnl3 GTPase activating RANGAP do... XM_011239224.2 74.8% 20% (many diffs)
28 mouse 99326 Garnl3 GTPase activating RANGAP do... XM_011239225.2 74.8% 20% (many diffs)
29 mouse 99326 Garnl3 GTPase activating RANGAP do... XM_017319358.1 74.8% 20% (many diffs)
30 mouse 99326 Garnl3 GTPase activating RANGAP do... XR_001783216.1 44.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1152
ORF length:
1086
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa gcatttttgt tccagctctg tctcggaaga cctaggctgt agacgtgggg
121atttcagtag gaaacattat ggatctgtgg agctgcttat ttccagtgat gctgatggag
181ccatccaaag ggctggaaga ttcagagtgg aaaatggctc ttcagatgag aatgcaactg
241ccctgcctgg tacttggcga agaacagacg tgcacttaga gaacccagaa taccacacca
301gatggtattt caaatatttt ttaggacaag tccgtcagaa ctacattgga aacgatgccg
361agaagagccc tttcttcttg tccgtgaccc tttctgacca aaacaatcaa cgtgtccctc
421aataccgtgc aattctttgg agaaaaacag gtacccagaa aatatgcctt ccctacagtc
481ccacaaaaac tctttctgtg aagtccatct taagtgccat gaatctggac aaatttgaga
541aaggccccag ggaaattttt catcctgaaa tacaaaagga cttgctggtt cttgaagaac
601aagagggctc tgtgaatttc aagtttgggg ttctttttgc caaagatggg cagctcactg
661atgatgagat gttcagcaat gaaattggaa gcgagccttt tcaaaaattt ttaaatcttc
721tgggtgacac aatcactcta aagggctgga cgggctaccg tggcggtctg gataccaaaa
781atgataccac agggatacat tcagtttata ctgtgtacca agggcatgag atcatgtttc
841atgtttccac catgttgcca tattccaaag agaacaaaca gcaggtggaa aggaaacgcc
901acattggaaa cgatatcgtc accattgtgt tccaagaagg agaggaatct tctcctgcct
961ttaagccttc catgatccgc tcccacttta cacatatttt tgccttagtg agatacaatc
1021aacaaaatga caattacagg ctgaaaatat tttcagaaga gagcgtacca ctctttggcc
1081ctcccttgcc aactccacca gtgtttacag accaccagga attcagggat ctttttgcta
1141gtgaaattaa ttaatggtga aaaagccact ttggaaaccc caacatttgc ccagaaacgt
1201cggcgtaccc tggatatgtt gattagatct ttacaccagg atttgatgcc agatttgcat
1261aagaacatgc ttaatagacg atcttttagt gatgtcttac cagagtcacc caagtcagcg
1321cggaagaaag aggaggcccg ccaggcggag tttgttagaa tagggcaggc actaaaactg
1381aaatccattg tgagagggga tgctccatca agcttggcag cttcagggat ctgtaaaaaa
1441gagccgtggg agccccagtg tttctgcagt aatttccctc atgaagccgt gtgtgcagat
1501ccctggggcc aggccttgct ggtttccact gatgctggcg tcttgctagt ggatgatgac
1561cttccatcag tgcccgtgtt tgacagaact ctgccagtga agcaaatgcg tgtgcttgag
1621accctggacc ttctggttct cagagcagac aaaggaaaag atgctcgcct ctttgtcttc
1681aggctaagtg ctctgcaaaa gggccttgag gggaagcagg ctgggaagag caggtctgac
1741tgcagagaaa acaagttgga gaaaacaaaa ggctgccacc tgtatgctat taacactcac
1801cacagcagag agctgaggat tgtggttgca attcggaata aactgcttct gatcacaaga
1861aaacacaaca agccaagcgg ggtcaccagc acctcattgt tatctcccct gtctgagtca
1921cctgttgaag aattccagta catcagggag atctgtctgt ctgactctcc catggtgatg
1981accttagtgg atgggccagc tgaagagagt gacaatctca tctgtgtggc ttatcgacac
2041caatttgatg tggtgaatga gagcacagga gaagccttca ggctgcacca cgtggaggcc
2101aacagggtta attttgttgc agctattgat gtgtacgaag atggagaagc tggtttgctg
2161ttgtgttaca actacagttg catctataaa aaggtttgcc cctttaatgg tggctctttt
2221ttggttcaac cttctgcgtc agatttccag ttctgttgga accaggctcc ctatgcaatt
2281gtctgtgctt tcccgtatct cctggccttc accaccgact ccatggagat ccgcctggtg
2341gtgaacggga acctggtcca cactgcagtc gtgccgcagc tgcagctggt ggcctccagg
2401tcggatatat acttcacagc aactgcagct gtgaatgagg tctcatctgg aggcagctcc
2461aagggggcca gtgcccgaaa ttctcctcag acacccccgg gccgagatac tccagtattt
2521ccttcttccc tgggggaagg tgaaattcaa tcaaaaaatc tgtacaagat tccacttaga
2581aacctcgtgg gcagaagcat cgaacgacct ctgaagtcac ccttagtctc caaggtcatc
2641accccaccca ctcccatcca gtgtgggcct tgctgccatt ccagtcacgc actccttgtc
2701cctgtctcgc atggagatca aagaaatagc aagcaggacc cgcagggaac tactgggcct
2761ctcggatgaa ggtggaccca agtcagaagg agcgccaaag gccaaatcaa aaccccggaa
2821gcggttagaa gaaagccaag gaggccccaa gccaggggca gtgaggtcat ctagcagtga
2881caggatccca tcaggctcct tggaaagtgc ttctacttcc gaagccaacc ctgaggggca
2941ctcagccagc tctgaccagg accctgtggc agacagagag ggcagcccgg tctccggcag
3001cagccccttc cagctcacgg ctttctccga tgaagacatt atagacttga agtacccaac
3061tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc tcgattctac
3121gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc tctggatta

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