Construct: ORF ccsbBroad304_15192
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014038.1_s304c1, BRDN0000462243
- Derived from:
- ccsbBroadEn_15192
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- GARNL3 (84253)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15192
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84253 | GARNL3 | GTPase activating Rap/RanGA... | NM_001286779.1 | 99.4% | 35.2% | (many diffs) |
| 2 | human | 84253 | GARNL3 | GTPase activating Rap/RanGA... | NM_032293.5 | 98% | 35.2% | (many diffs) |
| 3 | human | 84253 | GARNL3 | GTPase activating Rap/RanGA... | XM_017015202.1 | 96.4% | 34.2% | (many diffs) |
| 4 | human | 84253 | GARNL3 | GTPase activating Rap/RanGA... | XM_005252268.2 | 95.6% | 33.7% | (many diffs) |
| 5 | human | 84253 | GARNL3 | GTPase activating Rap/RanGA... | XM_011519089.3 | 95.6% | 33.7% | (many diffs) |
| 6 | human | 84253 | GARNL3 | GTPase activating Rap/RanGA... | XM_024447693.1 | 95.6% | 33.7% | (many diffs) |
| 7 | human | 84253 | GARNL3 | GTPase activating Rap/RanGA... | XM_011519086.3 | 93.3% | 32.9% | (many diffs) |
| 8 | human | 84253 | GARNL3 | GTPase activating Rap/RanGA... | XM_011519087.2 | 93.3% | 32.9% | (many diffs) |
| 9 | human | 84253 | GARNL3 | GTPase activating Rap/RanGA... | XM_005252267.3 | 92.1% | 32.5% | (many diffs) |
| 10 | human | 84253 | GARNL3 | GTPase activating Rap/RanGA... | XM_011519090.2 | 84.7% | 20.8% | (many diffs) |
| 11 | human | 84253 | GARNL3 | GTPase activating Rap/RanGA... | XM_024447694.1 | 84.7% | 20.8% | (many diffs) |
| 12 | human | 84253 | GARNL3 | GTPase activating Rap/RanGA... | XM_024447695.1 | 84.7% | 20.8% | (many diffs) |
| 13 | human | 84253 | GARNL3 | GTPase activating Rap/RanGA... | XM_024447696.1 | 84.7% | 20.8% | (many diffs) |
| 14 | human | 84253 | GARNL3 | GTPase activating Rap/RanGA... | XR_001746389.1 | 79.7% | (many diffs) | |
| 15 | human | 84253 | GARNL3 | GTPase activating Rap/RanGA... | NR_104591.1 | 75.7% | (many diffs) | |
| 16 | human | 84253 | GARNL3 | GTPase activating Rap/RanGA... | XM_024447697.1 | 55.9% | 28.5% | (many diffs) |
| 17 | mouse | 99326 | Garnl3 | GTPase activating RANGAP do... | NM_178888.4 | 88% | 34.2% | (many diffs) |
| 18 | mouse | 99326 | Garnl3 | GTPase activating RANGAP do... | XM_006498486.3 | 86.1% | 33.5% | (many diffs) |
| 19 | mouse | 99326 | Garnl3 | GTPase activating RANGAP do... | XM_006498483.3 | 84.6% | 32.7% | (many diffs) |
| 20 | mouse | 99326 | Garnl3 | GTPase activating RANGAP do... | XM_006498484.3 | 84.6% | 32.7% | (many diffs) |
| 21 | mouse | 99326 | Garnl3 | GTPase activating RANGAP do... | XM_006498485.3 | 84.6% | 32.7% | (many diffs) |
| 22 | mouse | 99326 | Garnl3 | GTPase activating RANGAP do... | XM_006498482.3 | 81.8% | 31.6% | (many diffs) |
| 23 | mouse | 99326 | Garnl3 | GTPase activating RANGAP do... | XM_006498481.3 | 81.5% | 31.5% | (many diffs) |
| 24 | mouse | 99326 | Garnl3 | GTPase activating RANGAP do... | XM_006498487.3 | 74.8% | 20% | (many diffs) |
| 25 | mouse | 99326 | Garnl3 | GTPase activating RANGAP do... | XM_006498488.3 | 74.8% | 20% | (many diffs) |
| 26 | mouse | 99326 | Garnl3 | GTPase activating RANGAP do... | XM_011239223.2 | 74.8% | 20% | (many diffs) |
| 27 | mouse | 99326 | Garnl3 | GTPase activating RANGAP do... | XM_011239224.2 | 74.8% | 20% | (many diffs) |
| 28 | mouse | 99326 | Garnl3 | GTPase activating RANGAP do... | XM_011239225.2 | 74.8% | 20% | (many diffs) |
| 29 | mouse | 99326 | Garnl3 | GTPase activating RANGAP do... | XM_017319358.1 | 74.8% | 20% | (many diffs) |
| 30 | mouse | 99326 | Garnl3 | GTPase activating RANGAP do... | XR_001783216.1 | 44.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1152
- ORF length:
- 1086
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcatttttgt tccagctctg tctcggaaga cctaggctgt agacgtgggg 121 atttcagtag gaaacattat ggatctgtgg agctgcttat ttccagtgat gctgatggag 181 ccatccaaag ggctggaaga ttcagagtgg aaaatggctc ttcagatgag aatgcaactg 241 ccctgcctgg tacttggcga agaacagacg tgcacttaga gaacccagaa taccacacca 301 gatggtattt caaatatttt ttaggacaag tccgtcagaa ctacattgga aacgatgccg 361 agaagagccc tttcttcttg tccgtgaccc tttctgacca aaacaatcaa cgtgtccctc 421 aataccgtgc aattctttgg agaaaaacag gtacccagaa aatatgcctt ccctacagtc 481 ccacaaaaac tctttctgtg aagtccatct taagtgccat gaatctggac aaatttgaga 541 aaggccccag ggaaattttt catcctgaaa tacaaaagga cttgctggtt cttgaagaac 601 aagagggctc tgtgaatttc aagtttgggg ttctttttgc caaagatggg cagctcactg 661 atgatgagat gttcagcaat gaaattggaa gcgagccttt tcaaaaattt ttaaatcttc 721 tgggtgacac aatcactcta aagggctgga cgggctaccg tggcggtctg gataccaaaa 781 atgataccac agggatacat tcagtttata ctgtgtacca agggcatgag atcatgtttc 841 atgtttccac catgttgcca tattccaaag agaacaaaca gcaggtggaa aggaaacgcc 901 acattggaaa cgatatcgtc accattgtgt tccaagaagg agaggaatct tctcctgcct 961 ttaagccttc catgatccgc tcccacttta cacatatttt tgccttagtg agatacaatc 1021 aacaaaatga caattacagg ctgaaaatat tttcagaaga gagcgtacca ctctttggcc 1081 ctcccttgcc aactccacca gtgtttacag accaccagga attcagggat ctttttgcta 1141 gtgaaattaa ttaatggtga aaaagccact ttggaaaccc caacatttgc ccagaaacgt 1201 cggcgtaccc tggatatgtt gattagatct ttacaccagg atttgatgcc agatttgcat 1261 aagaacatgc ttaatagacg atcttttagt gatgtcttac cagagtcacc caagtcagcg 1321 cggaagaaag aggaggcccg ccaggcggag tttgttagaa tagggcaggc actaaaactg 1381 aaatccattg tgagagggga tgctccatca agcttggcag cttcagggat ctgtaaaaaa 1441 gagccgtggg agccccagtg tttctgcagt aatttccctc atgaagccgt gtgtgcagat 1501 ccctggggcc aggccttgct ggtttccact gatgctggcg tcttgctagt ggatgatgac 1561 cttccatcag tgcccgtgtt tgacagaact ctgccagtga agcaaatgcg tgtgcttgag 1621 accctggacc ttctggttct cagagcagac aaaggaaaag atgctcgcct ctttgtcttc 1681 aggctaagtg ctctgcaaaa gggccttgag gggaagcagg ctgggaagag caggtctgac 1741 tgcagagaaa acaagttgga gaaaacaaaa ggctgccacc tgtatgctat taacactcac 1801 cacagcagag agctgaggat tgtggttgca attcggaata aactgcttct gatcacaaga 1861 aaacacaaca agccaagcgg ggtcaccagc acctcattgt tatctcccct gtctgagtca 1921 cctgttgaag aattccagta catcagggag atctgtctgt ctgactctcc catggtgatg 1981 accttagtgg atgggccagc tgaagagagt gacaatctca tctgtgtggc ttatcgacac 2041 caatttgatg tggtgaatga gagcacagga gaagccttca ggctgcacca cgtggaggcc 2101 aacagggtta attttgttgc agctattgat gtgtacgaag atggagaagc tggtttgctg 2161 ttgtgttaca actacagttg catctataaa aaggtttgcc cctttaatgg tggctctttt 2221 ttggttcaac cttctgcgtc agatttccag ttctgttgga accaggctcc ctatgcaatt 2281 gtctgtgctt tcccgtatct cctggccttc accaccgact ccatggagat ccgcctggtg 2341 gtgaacggga acctggtcca cactgcagtc gtgccgcagc tgcagctggt ggcctccagg 2401 tcggatatat acttcacagc aactgcagct gtgaatgagg tctcatctgg aggcagctcc 2461 aagggggcca gtgcccgaaa ttctcctcag acacccccgg gccgagatac tccagtattt 2521 ccttcttccc tgggggaagg tgaaattcaa tcaaaaaatc tgtacaagat tccacttaga 2581 aacctcgtgg gcagaagcat cgaacgacct ctgaagtcac ccttagtctc caaggtcatc 2641 accccaccca ctcccatcca gtgtgggcct tgctgccatt ccagtcacgc actccttgtc 2701 cctgtctcgc atggagatca aagaaatagc aagcaggacc cgcagggaac tactgggcct 2761 ctcggatgaa ggtggaccca agtcagaagg agcgccaaag gccaaatcaa aaccccggaa 2821 gcggttagaa gaaagccaag gaggccccaa gccaggggca gtgaggtcat ctagcagtga 2881 caggatccca tcaggctcct tggaaagtgc ttctacttcc gaagccaacc ctgaggggca 2941 ctcagccagc tctgaccagg accctgtggc agacagagag ggcagcccgg tctccggcag 3001 cagccccttc cagctcacgg ctttctccga tgaagacatt atagacttga agtacccaac 3061 tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc tcgattctac 3121 gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc tctggatta