Transcript: Mouse XR_001782319.1

PREDICTED: Mus musculus leucyl-tRNA synthetase (Lars), transcript variant X1, misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Lars (107045)
Length:
3838
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001782319.1
NBCI Gene record:
Lars (107045)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_001782319.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000253379 GCCGGTTGTGAAGGAGTTAAT pLKO_005 1267 3UTR 100% 13.200 18.480 N Lars n/a
2 TRCN0000253380 CTCCCTATGCTGACCATTAAA pLKO_005 1352 3UTR 100% 15.000 10.500 N Lars n/a
3 TRCN0000253378 CTGTACTGGTATGCCTATTAA pLKO_005 502 3UTR 100% 15.000 10.500 N Lars n/a
4 TRCN0000253377 CGGATACTTCTGCTCCGAGAA pLKO_005 3702 3UTR 100% 4.050 2.835 N Lars n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001782319.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03323 pDONR223 100% 74.9% None (many diffs) n/a
2 ccsbBroad304_03323 pLX_304 0% 74.9% V5 (many diffs) n/a
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