Construct: ORF ccsbBroad304_03323
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002711.1_s304c1, BRDN0000462302
- Derived from:
- ccsbBroadEn_03323
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LARS1 (51520)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03323
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51520 | LARS1 | leucyl-tRNA synthetase 1 | NM_020117.11 | 100% | 100% | |
| 2 | human | 51520 | LARS1 | leucyl-tRNA synthetase 1 | NM_016460.3 | 97.7% | 97.7% | 211_212ins81 |
| 3 | human | 51520 | LARS1 | leucyl-tRNA synthetase 1 | NM_001317964.1 | 96% | 96% | 291_292ins138 |
| 4 | human | 51520 | LARS1 | leucyl-tRNA synthetase 1 | NM_001317965.1 | 95.4% | 95.4% | 0_1ins162 |
| 5 | human | 51520 | LARS1 | leucyl-tRNA synthetase 1 | XM_011537656.3 | 95.4% | 95.4% | 0_1ins162 |
| 6 | mouse | 107045 | Lars | leucyl-tRNA synthetase | NM_134137.2 | 85.8% | 90% | (many diffs) |
| 7 | mouse | 107045 | Lars | leucyl-tRNA synthetase | XR_001782319.1 | 74.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3597
- ORF length:
- 3528
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggaaaga aaaggaacag ccaaagtgga ctttttgaag aagattgaga 121 aagaaatcca acagaaatgg gatactgaga gagtgtttga ggtcaatgca tctaatttag 181 agaaacagac cagcaagggc aagtattttg taaccttccc atatccatat atgaatggac 241 gccttcattt gggacacacg ttttctttat ccaaatgtga gtttgctgta gggtaccagc 301 gattgaaagg aaaatgttgt ctgtttccct ttggcctgca ctgtactgga atgcctatta 361 aggcatgtgc tgataagttg aaaagagaaa tagagctgta tggttgcccc cctgattttc 421 cagatgaaga agaggaagag gaagaaacca gtgttaaaac agaagatata ataattaagg 481 ataaagctaa aggaaaaaag agtaaagctg ctgctaaagc tggatcttct aaataccagt 541 ggggcattat gaaatccctt ggcctgtctg atgaagagat agtaaaattt tctgaagcag 601 aacattggct tgattatttc ccgccactgg ctattcagga tttaaaaaga atgggtttga 661 aggtagactg gcgtcgttcc ttcatcacca ctgatgttaa tccttactat gattcatttg 721 tcagatggca atttttaaca ttaagagaaa gaaacaaaat taaatttggg aagcggtata 781 caatttactc tccgaaagat ggacagcctt gcatggatca tgatagacaa actggagagg 841 gtgttggacc tcaggaatat actttactca aattgaaggt gcttgagcca tacccatcta 901 aattaagtgg cctgaaaggt aaaaatattt tcttggtggc tgctactctc agacctgaga 961 ccatgtttgg gcagacaaat tgttgggttc gtcctgatat gaagtacatt ggatttgaga 1021 cggtgaatgg tgatatattc atctgtaccc aaaaagcagc caggaatatg tcataccagg 1081 gctttaccaa agacaatggc gtggtgcctg ttgttaagga attaatgggg gaggaaattc 1141 ttggtgcatc actttctgca cctttaacat catacaaggt gatctatgtt ctcccaatgc 1201 taactattaa ggaggataaa ggcactggtg tggttacaag tgttccttcc gactcccctg 1261 atgatattgc tgccctcaga gacttgaaga aaaagcaagc cttacgagca aaatatggaa 1321 ttagagatga catggtcttg ccatttgagc cggtgccagt cattgaaatc ccaggttttg 1381 gaaatctttc tgctgtaacc atttgtgatg agttgaaaat tcagagccag aatgaccggg 1441 aaaaacttgc agaagcaaag gagaagatat atctaaaagg attttatgag ggtatcatgt 1501 tggtggatgg atttaaagga cagaaggttc aagatgtaaa gaagactatt cagaaaaaga 1561 tgattgacgc tggagatgca cttatttaca tggaaccaga gaaacaagtg atgtccaggt 1621 cgtcagatga atgtgttgtg gctctgtgtg accagtggta cttggattat ggagaagaga 1681 attggaagaa acagacatct cagtgcttga agaacctgga aacattctgt gaggagacca 1741 ggaggaattt tgaagccacc ttaggttggc tacaagaaca tgcttgctca agaacttatg 1801 gtctaggcac tcacctgcct tgggatgagc agtggctgat tgaatcactt tctgactcca 1861 ctatttacat ggcattttac acagttgcac acctattgca ggggggtaac ttgcatggac 1921 aggcagagtc tccgctgggc attagaccgc aacagatgac caaggaagtt tgggattatg 1981 ttttcttcaa ggaggctcca tttcctaaga ctcagattgc aaaggaaaaa ttagatcagt 2041 taaagcagga gtttgaattc tggtatcctg ttgatcttcg cgtctctggc aaggatcttg 2101 ttccaaatca tctttcatat tacctttata atcatgtggc tatgtggccg gaacaaagtg 2161 acaaatggcc tacagctgtg agagcaaatg gacatctcct cctgaactct gagaagatgt 2221 caaaatccac aggcaacttc ctcactttga cccaagctat tgacaaattt tcagcagatg 2281 gaatgcgttt ggctctggct gatgctggtg acactgtaga agatgccaac tttgtggaag 2341 ccatggcaga tgcaggtatt ctccgtctgt acacctgggt agagtgggtg aaagaaatgg 2401 ttgccaactg ggacagccta agaagtggtc ctgccagcac tttcaatgat agagtttttg 2461 ccagtgaatt gaatgcagga attataaaaa cagatcaaaa ctatgaaaag atgatgttta 2521 aagaagcttt gaaaacaggg ttttttgagt ttcaggccgc aaaagataag taccgtgaat 2581 tggctgtgga agggatgcac agagaacttg tgttccggtt tattgaagtt cagacacttc 2641 tcctcgctcc attctgtcca catttgtgtg agcacatctg gacactcctg ggaaagcctg 2701 actcaattat gaatgcttca tggcctgtgg caggtcctgt taatgaagtt ttaatacact 2761 cctcacagta tcttatggaa gtaacacatg accttagact acgactcaag aactatatga 2821 tgccagctaa agggaagaag actgacaaac aacccctgca gaagccctca cattgcacca 2881 tctatgtggc aaagaactat ccaccttggc aacataccac cctgtctgtt ctacgtaaac 2941 actttgaggc caataacgga aaactgcctg acaacaaagt cattgctagt gaactaggca 3001 gtatgccaga actgaagaaa tacatgaaga aagtcatgcc atttgttgcc atgattaagg 3061 aaaatctgga gaagatgggg cctcgtattc tggatttgca attagaattt gatgaaaagg 3121 ctgtgcttat ggagaatata gtctatctga ctaattcgct tgagctagaa cacatagaag 3181 tcaagtttgc ctccgaagca gaagataaaa tcagggaaga ctgctgtcct gggaaaccac 3241 ttaatgtttt tagaatagaa cctggtgtgt ccgtttctct ggtgaatccc cagccatcca 3301 atggccactt ctcaaccaaa attgaaatca ggcaaggaga taactgtgat tccataatca 3361 ggcgtttaat gaaaatgaat cgaggaatta aagacctttc caaagtgaaa ctgatgagat 3421 ttgatgatcc actgttgggg cctcgacgag ttcctgtcct gggaaaggag tacaccgaga 3481 agacccccat ttctgagcat gctgttttca atgtggacct catgagcaag aaaattcatc 3541 tgactgagaa tgggataagg gtggatattg gcgatacaat aatctatctg gttcatttgc 3601 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 3661 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 3721 tta