Transcript: Human XR_002959585.1

PREDICTED: Homo sapiens queuine tRNA-ribosyltransferase accessory subunit 2 (QTRT2), transcript variant X8, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
QTRT2 (79691)
Length:
4047
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002959585.1
NBCI Gene record:
QTRT2 (79691)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002959585.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000034702 CCGATGGAGAAGTATCTTGTA pLKO.1 663 3UTR 100% 4.950 6.930 N QTRT2 n/a
2 TRCN0000034701 CCTGCTTATGATGCACAACTT pLKO.1 1484 3UTR 100% 4.950 6.930 N QTRT2 n/a
3 TRCN0000034700 CCAAGGCTCATATCTGGTGTT pLKO.1 971 3UTR 100% 4.050 3.240 N QTRT2 n/a
4 TRCN0000294093 GATATAAGGTCTGCTTAAATA pLKO_005 1707 3UTR 100% 15.000 10.500 N QTRT2 n/a
5 TRCN0000294092 TGAAGTCTTGACAGAATATAA pLKO_005 442 3UTR 100% 15.000 10.500 N QTRT2 n/a
6 TRCN0000306918 ACCCATCACACGCTGCATAAT pLKO_005 365 3UTR 100% 13.200 9.240 N QTRT2 n/a
7 TRCN0000294094 GGGACCACACCATGGATATTC pLKO_005 300 3UTR 100% 13.200 9.240 N QTRT2 n/a
8 TRCN0000034699 CCCGGCTGGTTATGTAACAAA pLKO.1 538 3UTR 100% 5.625 3.938 N QTRT2 n/a
9 TRCN0000430981 GCCACCATGCCTGGCTAATTT pLKO_005 3225 3UTR 100% 15.000 7.500 Y GTF2IRD2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002959585.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08946 pDONR223 100% 30.7% None (many diffs) n/a
2 ccsbBroad304_08946 pLX_304 0% 30.7% V5 (many diffs) n/a
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