Construct: ORF ccsbBroad304_08946
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013500.1_s304c1, BRDN0000403497
- Derived from:
- ccsbBroadEn_08946
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- QTRT2 (79691)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08946
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79691 | QTRT2 | queuine tRNA-ribosyltransfe... | NM_024638.4 | 99.9% | 99.7% | 16A>T |
| 2 | human | 79691 | QTRT2 | queuine tRNA-ribosyltransfe... | NM_001256835.2 | 97.1% | 96.9% | 1_36del;52A>T |
| 3 | human | 79691 | QTRT2 | queuine tRNA-ribosyltransfe... | XM_005247769.4 | 79.2% | 79.2% | 0_1ins258 |
| 4 | human | 79691 | QTRT2 | queuine tRNA-ribosyltransfe... | XM_006713744.4 | 79.2% | 79.2% | 0_1ins258 |
| 5 | human | 79691 | QTRT2 | queuine tRNA-ribosyltransfe... | XM_011513137.3 | 79.2% | 79.1% | 1_36del;52A>T;1053_1053delAins229 |
| 6 | human | 79691 | QTRT2 | queuine tRNA-ribosyltransfe... | NM_001256836.2 | 73.8% | 73.4% | 1_1delAins314;6_7insA;10_13delTGGCinsAACAAACA |
| 7 | human | 79691 | QTRT2 | queuine tRNA-ribosyltransfe... | NM_001256837.2 | 70.3% | 70.3% | 0_1ins369 |
| 8 | human | 79691 | QTRT2 | queuine tRNA-ribosyltransfe... | XM_005247770.3 | 70.3% | 70.3% | 0_1ins369 |
| 9 | human | 79691 | QTRT2 | queuine tRNA-ribosyltransfe... | XM_017007182.1 | 70.3% | 70.3% | 0_1ins369 |
| 10 | human | 79691 | QTRT2 | queuine tRNA-ribosyltransfe... | XM_017007183.2 | 70.3% | 70.3% | 0_1ins369 |
| 11 | human | 79691 | QTRT2 | queuine tRNA-ribosyltransfe... | XM_017007184.2 | 51.9% | 52% | 0_1ins369;648_648delAins229 |
| 12 | human | 79691 | QTRT2 | queuine tRNA-ribosyltransfe... | XR_001740268.2 | 31.6% | (many diffs) | |
| 13 | human | 79691 | QTRT2 | queuine tRNA-ribosyltransfe... | XR_002959585.1 | 30.7% | (many diffs) | |
| 14 | mouse | 106248 | Qtrt2 | queuine tRNA-ribosyltransfe... | XM_006521668.1 | 85.6% | 87.2% | (many diffs) |
| 15 | mouse | 106248 | Qtrt2 | queuine tRNA-ribosyltransfe... | XM_006521670.3 | 85.6% | 87.2% | (many diffs) |
| 16 | mouse | 106248 | Qtrt2 | queuine tRNA-ribosyltransfe... | NM_029128.2 | 71.1% | 72.3% | (many diffs) |
| 17 | mouse | 106248 | Qtrt2 | queuine tRNA-ribosyltransfe... | XM_011245795.2 | 68% | 69.8% | (many diffs) |
| 18 | mouse | 106248 | Qtrt2 | queuine tRNA-ribosyltransfe... | XM_011245796.2 | 60.3% | 62.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1311
- ORF length:
- 1245
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gctgagtctt tccaaggtag ttaatggctg tcgcctagga aaaataaaaa 121 acctgggcaa aacaggggac cacaccatgg atattccagg ctgccttctg tataccaaga 181 ctggctccgc cccacacctc acccatcaca cgctgcataa tatccacggg gttcctgcca 241 tggctcagct tacgctgtca tccctagcag aacatcatga agtcttgaca gaatataaag 301 aaggagttgg aaagtttata ggcatgccag aatcactctt gtactgctcc ctgcacgatc 361 cagtcagccc ctgcccggct ggttatgtaa caaacaagtc tgtgtctgtg tggagtgttg 421 caggacgagt ggaaatgact gtttccaagt tcatggcaat tcagaaggcc cttcagccag 481 actggttcca gtgcctctcc gatggagaag tatcttgtaa ggaagcaact tccataaaaa 541 gggtcagaaa gtctgttgac cgatcacttc ttttcttgga taactgtctg cggctgcagg 601 aagagtcaga ggttcttcag aagagtgtga tcattggagt gattgaaggt ggagatgtga 661 tggaagagag gctgaggtca gcacgagaga cagccaagcg gcctgtgggt ggcttccttc 721 tggatggttt tcaaggaaat ccaacaaccc tggaggctag actacgcttg ctgtcatcag 781 tcactgcaga gctgccggag gacaagccaa ggctcatatc tggtgttagt cggccagatg 841 aggtgctcga gtgtattgaa agaggagtgg acttatttga gagttttttc ccttatcaag 901 taacagagcg gggatgtgcc ctgactttca gttttgatta ccagccgaat cctgaagaga 961 cactactaca acaaaatgga acacaagaag aaataaaatg tatggatcaa ataaagaaaa 1021 ttgaaacaac tggttgcaac caagaaataa catcatttga aattaatctg aaggaaaaaa 1081 agtaccagga ggactttaac ccgctggtga gaggatgttc ctgttactgc tgtaagaatc 1141 acactcgggc atacatccac catctgctgg tgaccaatga gctgctggcc ggagtcctgc 1201 ttatgatgca caactttgaa cactactttg ggtttttcca ttacatccgg gaagcactaa 1261 aaagtgacaa actggcacag ttgaaagagc tcatccacag gcaagcatct tgcccaactt 1321 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1381 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta