Vector Details: pLX

Vector: pLX_317

pLX_317 is not available for distribution as a Gateway Destination vector, and users should request pLX_307 instead. This is because pLX_317, by definition, is pLX_307 plus a barcode sequence, but this barcode is added in the same workflow as the ORF insert. Thus, we only have individual clones of pLX_317 with both a barcode and an ORF insert.

View vector index

Vector Information:

Vector Name:: pLX_317
Alias:: pLX_TRC307WBC, pLX_TRC317
Pol II Cassette 1:: SV40-PuroR
Pol II Cassette 2:: EF1a-Gateway-V5(c-term)
Selection Marker:: PuroR
Visible Reporter:: n/a
Cloning Site:: n/a
Epitope Tag:: n/a

Sequence Data Files

For a free vector file viewer, try ApE or SnapGene Viewer.

	GenBank[?]File in GenBank flat-file format	FASTA	SnapGene[?]File in proprietary SnapGene .DNA format	Revision Notes
1	GB	FASTA	DNA	(original version)

GenBank[?]File in GenBank flat-file format

FASTA

SnapGene[?]File in proprietary SnapGene .DNA format

Revision Notes

FASTA

DNA

(original version)

Download CSV

Control Clones

	Clone ID	Sequenced %[?]Percentage of construct sequence that has been empirically verified through direct sequence data.	Perfect Match Genes[?]100% full-length nucleotide and amino acid (for coding transcripts) alignment to current RefSeq transcript.	Near Match Genes[?]At least 99% full-length nucleotide and amino acid (for coding transcripts) alignment to current RefSeq transcript.	Lower Match Genes[?]At least 50% full-length nucleotide and amino acid (for coding transcripts) alignment to current RefSeq transcript, with at least 90% sequence identity over aligned region.	Addgene[?]Link to this entity's page in the Addgene catalog, if available
1	BRDN0000559468	30.3%	LUCIFERASE, Luciferase			n/a
2	BRDN0000559460	61.8%	BFP		GFP, eGFP	n/a
3	BRDN0000559463	72.2%	HcRed			n/a
4	BRDN0000559461	14.2%	LacZ		lacZ	n/a
5	BRDN0000559466	70%	eGFP	GFP	BFP	n/a

Clone ID

Sequenced %[?]Percentage of construct sequence that has been empirically verified through direct sequence data.

Perfect Match Genes[?]100% full-length nucleotide and amino acid (for coding transcripts) alignment to current RefSeq transcript.

Near Match Genes[?]At least 99% full-length nucleotide and amino acid (for coding transcripts) alignment to current RefSeq transcript.

Lower Match Genes[?]At least 50% full-length nucleotide and amino acid (for coding transcripts) alignment to current RefSeq transcript, with at least 90% sequence identity over aligned region.

Notes

Addgene[?]Link to this entity's page in the Addgene catalog, if available

BRDN0000559468

30.3%

LUCIFERASE, Luciferase

n/a

BRDN0000559460

61.8%

BFP

GFP, eGFP

n/a

BRDN0000559463

72.2%

HcRed

n/a

BRDN0000559461

14.2%

LacZ

lacZ

n/a

BRDN0000559466

70%

eGFP

GFP

BFP

n/a

Download CSV

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