Construct: sgRNA BRDN0001144725
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAACATCTTGACCACCACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SCYL1 (57410)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76066
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000011.10 |
11 |
65531626 |
- |
SCYL1 |
NNNACATCTTGACCACCACA |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000001.11 |
1 |
65366141 |
+ |
DNAJC6 |
NNNACATCTTGACCACTACA |
NAG |
2 |
0.121 |
Tier I |
3 |
NC_000009.12 |
9 |
36966573 |
- |
PAX5 |
NNNACATCTTCACCACCACA |
NAG |
2 |
0.1111 |
Tier I |
4 |
NC_000008.11 |
8 |
108482155 |
+ |
EMC2 |
NNNACATCTTCACCAACACA |
NGG |
2 |
0.4286 |
Tier II |
5 |
NC_000010.11 |
10 |
77465854 |
- |
KCNMA1 |
NNNACATCTAGACCACCATA |
NGG |
2 |
0.3956 |
Tier II |
6 |
NC_000009.12 |
9 |
18742922 |
+ |
ADAMTSL1 |
NNNTGATCTTGACCACCACA |
NGG |
2 |
0.3818 |
Tier II |
7 |
NC_000010.11 |
10 |
54276497 |
- |
PCDH15 |
NNNACATTTTCACCACCACA |
NGG |
2 |
0.375 |
Tier II |
8 |
NC_000002.12 |
2 |
191304104 |
- |
MYO1B |
NNNAGAGCTTGACCACCACA |
NGG |
2 |
0.3529 |
Tier II |
9 |
NC_000010.11 |
10 |
131295448 |
+ |
TCERG1L |
NNNACATCTTCACCACCACG |
NGG |
2 |
0.3277 |
Tier II |
10 |
NC_000001.11 |
1 |
231968368 |
+ |
DISC1 |
NNNACATCTGGACCACAACA |
NGG |
2 |
0.2333 |
Tier II |
11 |
NC_000022.11 |
22 |
29220914 |
- |
EMID1 |
NNNACATCTTGACCTCCACA |
NGG |
1 |
0.2 |
Tier II |
12 |
NC_000002.12 |
2 |
45838118 |
- |
PRKCE |
NNNACACCTTGACCACCCCA |
NGG |
2 |
0.131 |
Tier II |
13 |
NC_000017.11 |
17 |
79153275 |
- |
RBFOX3 |
NNNACATCTTGGCCAGCACA |
NGG |
2 |
0.1111 |
Tier II |
14 |
NC_000002.12 |
2 |
229065813 |
+ |
PID1 |
NNNCCATCTTGACCACCACA |
NAG |
2 |
0.0915 |
Tier II |
15 |
NC_000008.11 |
8 |
97137946 |
- |
CPQ |
NNNACATCTTGACCTCCAGA |
NGG |
2 |
0.025 |
Tier II |
16 |
NC_000020.11 |
20 |
49333601 |
- |
LOC105372649 |
NNNCCATCTTGACCACCACA |
NGG |
1 |
0.3529 |
Tier III |
17 |
NC_000008.11 |
8 |
41178899 |
+ |
LOC105379771 |
NNNACATCTAGTCCACCACA |
NGG |
2 |
0.2857 |
Tier III |
18 |
NC_000010.11 |
10 |
31949741 |
+ |
LOC107984219 |
NNNACATTTTGACCATCACA |
NGG |
2 |
0.2692 |
Tier III |
19 |
NC_000001.11 |
1 |
231968368 |
+ |
TSNAX-DISC1 |
NNNACATCTGGACCACAACA |
NGG |
2 |
0.2333 |
Tier III |
20 |
NC_000019.10 |
19 |
27947132 |
- |
LINC02987 |
NNNACATCCTGAGCACCACA |
NGG |
2 |
0.1259 |
Tier III |
21 |
NC_000008.11 |
8 |
97137946 |
- |
LOC101927066 |
NNNACATCTTGACCTCCAGA |
NGG |
2 |
0.025 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000070.6 |
4 |
101599188 |
+ |
Dnajc6 |
NNNACATCTTGACCACTACA |
NAG |
2 |
0.121 |
Tier I |
2 |
NC_000083.6 |
17 |
71310871 |
+ |
Emilin2 |
NNNACATCTTGAGCCCCACA |
NGG |
2 |
0.0372 |
Tier I |
3 |
NC_000076.6 |
10 |
28245866 |
- |
Ptprk |
NNNACAACTAGACCACCACA |
NGG |
2 |
0.75 |
Tier II |
4 |
NC_000073.6 |
7 |
119599857 |
- |
Acsm2 |
NNNACATGATGACCACCACA |
NGG |
2 |
0.5969 |
Tier II |
5 |
NC_000081.6 |
15 |
86447076 |
+ |
Tbc1d22a |
NNNAGATCCTGACCACCACA |
NGG |
2 |
0.5538 |
Tier II |
6 |
NC_000070.6 |
4 |
6695732 |
+ |
Tox |
NNNACATTGTGACCACCACA |
NGG |
2 |
0.5417 |
Tier II |
7 |
NC_000082.6 |
16 |
38318823 |
+ |
Maats1 |
NNNGCATCTTGACCACCTCA |
NGG |
2 |
0.3125 |
Tier II |
8 |
NC_000067.6 |
1 |
132536771 |
- |
Cntn2 |
NNNAAATCTTGACCACTACA |
NGG |
2 |
0.2667 |
Tier II |
9 |
NC_000070.6 |
4 |
128703133 |
+ |
Phc2 |
NNNACATCTGGACCACCAAA |
NGG |
2 |
0.2143 |
Tier II |
10 |
NC_000070.6 |
4 |
128703273 |
+ |
Phc2 |
NNNACATCTGGACCACCAAA |
NGG |
2 |
0.2143 |
Tier II |
11 |
NC_000074.6 |
8 |
69423712 |
- |
Gm9495 |
NNNACATCTTGACCTACACA |
NGG |
2 |
0.2 |
Tier II |
12 |
NC_000080.6 |
14 |
30962322 |
+ |
Nek4 |
NNNACATCTTGACCTACACA |
NGG |
2 |
0.2 |
Tier II |
13 |
NC_000080.6 |
14 |
65861624 |
+ |
Ccdc25 |
NNNACATCTTGACTACCACA |
NAG |
2 |
0.1901 |
Tier II |
14 |
NC_000077.6 |
11 |
8535755 |
+ |
Tns3 |
NNNACATCTTGACCACTGCA |
NGG |
2 |
0.1867 |
Tier II |
15 |
NC_000077.6 |
11 |
47895583 |
+ |
Sgcd |
NNNACATCTGGTCCACCACA |
NGG |
2 |
0.1667 |
Tier II |
16 |
NC_000068.7 |
2 |
158820563 |
- |
Dhx35 |
NNNACATCTAGAGCACCACA |
NGG |
2 |
0.1169 |
Tier II |
17 |
NC_000072.6 |
6 |
39996360 |
+ |
Tmem178b |
NNNACATCTTGACCACCTCC |
NGG |
2 |
0.1136 |
Tier II |
18 |
NC_000072.6 |
6 |
146441148 |
- |
Itpr2 |
NNNACATCTTTACCAGCACA |
NGG |
2 |
0.0592 |
Tier II |
19 |
NC_000071.6 |
5 |
31076391 |
+ |
Cad |
NNNCCATCTTGACCAGCACA |
NGG |
2 |
0.0543 |
Tier II |
20 |
NC_000075.6 |
9 |
62531232 |
- |
Coro2b |
NNNACATCTTGTCCAGCACA |
NGG |
2 |
0.0513 |
Tier II |
21 |
NC_000080.6 |
14 |
59456449 |
- |
Cab39l |
NNNACATCATGACCACCACA |
NTG |
2 |
0.0362 |
Tier II |
22 |
NC_000076.6 |
10 |
36984042 |
+ |
Hdac2 |
NNNACATCGTGACCACCACA |
NGC |
2 |
0.0138 |
Tier II |
23 |
NC_000069.6 |
3 |
126979428 |
- |
Ank2 |
NNNACTTCTTGACCACCACA |
NGT |
2 |
0.0115 |
Tier II |
24 |
NC_000077.6 |
11 |
59105212 |
+ |
Obscn |
NNNACATCTGGACCACCACA |
NGC |
2 |
0.0111 |
Tier II |
25 |
NC_000071.6 |
5 |
119078435 |
- |
Gm31034 |
NNNACATCTTGACCACCACA |
NGG |
0 |
1.0 |
Tier III |
26 |
NC_000078.6 |
12 |
25160663 |
- |
Gm36287 |
NNNACATCATGACCAACACA |
NGG |
2 |
0.9286 |
Tier III |
27 |
NC_000082.6 |
16 |
73314398 |
- |
4930500H12Rik |
NNNACATTCTGACCACCACA |
NGG |
2 |
0.8077 |
Tier III |
28 |
NC_000073.6 |
7 |
41673983 |
- |
Vmn2r-ps54 |
NNNACATGTTGACCACCACT |
NGG |
2 |
0.3857 |
Tier III |
29 |
NC_000073.6 |
7 |
69606530 |
- |
Gm7627 |
NNNACATCTTCACCACCACA |
NAG |
2 |
0.1111 |
Tier III |
30 |
NC_000086.7 |
X |
90834929 |
- |
Bmp4-ps |
NNNATATCTTGACCACCAGA |
NGG |
2 |
0.0795 |
Tier III |
31 |
NC_000078.6 |
12 |
112268611 |
- |
Gm36894 |
NNNACATCTTGATCACCACA |
NGT |
2 |
0.0113 |
Tier III |
Other clones with same target sequence:
(none)