Construct: sgRNA BRDN0001144725

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAACATCTTGACCACCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCYL1 (57410)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76066

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 65531626 - SCYL1 NNNACATCTTGACCACCACA NGG 0 1.0 Tier I
2 NC_000001.11 1 65366141 + DNAJC6 NNNACATCTTGACCACTACA NAG 2 0.121 Tier I
3 NC_000009.12 9 36966573 - PAX5 NNNACATCTTCACCACCACA NAG 2 0.1111 Tier I
4 NC_000008.11 8 108482155 + EMC2 NNNACATCTTCACCAACACA NGG 2 0.4286 Tier II
5 NC_000010.11 10 77465854 - KCNMA1 NNNACATCTAGACCACCATA NGG 2 0.3956 Tier II
6 NC_000009.12 9 18742922 + ADAMTSL1 NNNTGATCTTGACCACCACA NGG 2 0.3818 Tier II
7 NC_000010.11 10 54276497 - PCDH15 NNNACATTTTCACCACCACA NGG 2 0.375 Tier II
8 NC_000002.12 2 191304104 - MYO1B NNNAGAGCTTGACCACCACA NGG 2 0.3529 Tier II
9 NC_000010.11 10 131295448 + TCERG1L NNNACATCTTCACCACCACG NGG 2 0.3277 Tier II
10 NC_000001.11 1 231968368 + DISC1 NNNACATCTGGACCACAACA NGG 2 0.2333 Tier II
11 NC_000022.11 22 29220914 - EMID1 NNNACATCTTGACCTCCACA NGG 1 0.2 Tier II
12 NC_000002.12 2 45838118 - PRKCE NNNACACCTTGACCACCCCA NGG 2 0.131 Tier II
13 NC_000017.11 17 79153275 - RBFOX3 NNNACATCTTGGCCAGCACA NGG 2 0.1111 Tier II
14 NC_000002.12 2 229065813 + PID1 NNNCCATCTTGACCACCACA NAG 2 0.0915 Tier II
15 NC_000008.11 8 97137946 - CPQ NNNACATCTTGACCTCCAGA NGG 2 0.025 Tier II
16 NC_000020.11 20 49333601 - LOC105372649 NNNCCATCTTGACCACCACA NGG 1 0.3529 Tier III
17 NC_000008.11 8 41178899 + LOC105379771 NNNACATCTAGTCCACCACA NGG 2 0.2857 Tier III
18 NC_000010.11 10 31949741 + LOC107984219 NNNACATTTTGACCATCACA NGG 2 0.2692 Tier III
19 NC_000001.11 1 231968368 + TSNAX-DISC1 NNNACATCTGGACCACAACA NGG 2 0.2333 Tier III
20 NC_000019.10 19 27947132 - LINC02987 NNNACATCCTGAGCACCACA NGG 2 0.1259 Tier III
21 NC_000008.11 8 97137946 - LOC101927066 NNNACATCTTGACCTCCAGA NGG 2 0.025 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 101599188 + Dnajc6 NNNACATCTTGACCACTACA NAG 2 0.121 Tier I
2 NC_000083.6 17 71310871 + Emilin2 NNNACATCTTGAGCCCCACA NGG 2 0.0372 Tier I
3 NC_000076.6 10 28245866 - Ptprk NNNACAACTAGACCACCACA NGG 2 0.75 Tier II
4 NC_000073.6 7 119599857 - Acsm2 NNNACATGATGACCACCACA NGG 2 0.5969 Tier II
5 NC_000081.6 15 86447076 + Tbc1d22a NNNAGATCCTGACCACCACA NGG 2 0.5538 Tier II
6 NC_000070.6 4 6695732 + Tox NNNACATTGTGACCACCACA NGG 2 0.5417 Tier II
7 NC_000082.6 16 38318823 + Maats1 NNNGCATCTTGACCACCTCA NGG 2 0.3125 Tier II
8 NC_000067.6 1 132536771 - Cntn2 NNNAAATCTTGACCACTACA NGG 2 0.2667 Tier II
9 NC_000070.6 4 128703133 + Phc2 NNNACATCTGGACCACCAAA NGG 2 0.2143 Tier II
10 NC_000070.6 4 128703273 + Phc2 NNNACATCTGGACCACCAAA NGG 2 0.2143 Tier II
11 NC_000074.6 8 69423712 - Gm9495 NNNACATCTTGACCTACACA NGG 2 0.2 Tier II
12 NC_000080.6 14 30962322 + Nek4 NNNACATCTTGACCTACACA NGG 2 0.2 Tier II
13 NC_000080.6 14 65861624 + Ccdc25 NNNACATCTTGACTACCACA NAG 2 0.1901 Tier II
14 NC_000077.6 11 8535755 + Tns3 NNNACATCTTGACCACTGCA NGG 2 0.1867 Tier II
15 NC_000077.6 11 47895583 + Sgcd NNNACATCTGGTCCACCACA NGG 2 0.1667 Tier II
16 NC_000068.7 2 158820563 - Dhx35 NNNACATCTAGAGCACCACA NGG 2 0.1169 Tier II
17 NC_000072.6 6 39996360 + Tmem178b NNNACATCTTGACCACCTCC NGG 2 0.1136 Tier II
18 NC_000072.6 6 146441148 - Itpr2 NNNACATCTTTACCAGCACA NGG 2 0.0592 Tier II
19 NC_000071.6 5 31076391 + Cad NNNCCATCTTGACCAGCACA NGG 2 0.0543 Tier II
20 NC_000075.6 9 62531232 - Coro2b NNNACATCTTGTCCAGCACA NGG 2 0.0513 Tier II
21 NC_000080.6 14 59456449 - Cab39l NNNACATCATGACCACCACA NTG 2 0.0362 Tier II
22 NC_000076.6 10 36984042 + Hdac2 NNNACATCGTGACCACCACA NGC 2 0.0138 Tier II
23 NC_000069.6 3 126979428 - Ank2 NNNACTTCTTGACCACCACA NGT 2 0.0115 Tier II
24 NC_000077.6 11 59105212 + Obscn NNNACATCTGGACCACCACA NGC 2 0.0111 Tier II
25 NC_000071.6 5 119078435 - Gm31034 NNNACATCTTGACCACCACA NGG 0 1.0 Tier III
26 NC_000078.6 12 25160663 - Gm36287 NNNACATCATGACCAACACA NGG 2 0.9286 Tier III
27 NC_000082.6 16 73314398 - 4930500H12Rik NNNACATTCTGACCACCACA NGG 2 0.8077 Tier III
28 NC_000073.6 7 41673983 - Vmn2r-ps54 NNNACATGTTGACCACCACT NGG 2 0.3857 Tier III
29 NC_000073.6 7 69606530 - Gm7627 NNNACATCTTCACCACCACA NAG 2 0.1111 Tier III
30 NC_000086.7 X 90834929 - Bmp4-ps NNNATATCTTGACCACCAGA NGG 2 0.0795 Tier III
31 NC_000078.6 12 112268611 - Gm36894 NNNACATCTTGATCACCACA NGT 2 0.0113 Tier III
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Other clones with same target sequence:

(none)