Construct: sgRNA BRDN0001145239
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGCTTTAGAGTACTTGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PDPK1 (5170)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77452
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
2565674 |
+ |
PDPK1 |
NNNGCTTTAGAGTACTTGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
79128851 |
- |
RBFOX3 |
NNNGCTTTAAAGTACCTGCA |
NGG |
2 |
0.6222 |
Tier II |
| 3 |
NC_000005.10 |
5 |
5272973 |
+ |
ADAMTS16 |
NNNACTTTGGAGTACTTGCA |
NGG |
2 |
0.6 |
Tier II |
| 4 |
NC_000007.14 |
7 |
142949210 |
- |
KEL |
NNNGCCTTAGAGTACTTGAA |
NGG |
2 |
0.3896 |
Tier II |
| 5 |
NC_000009.12 |
9 |
97662641 |
+ |
NCBP1 |
NNNGCTTTATATTACTTGCA |
NGG |
2 |
0.3125 |
Tier II |
| 6 |
NC_000009.12 |
9 |
97662641 |
+ |
XPA |
NNNGCTTTATATTACTTGCA |
NGG |
2 |
0.3125 |
Tier II |
| 7 |
NC_000011.10 |
11 |
33099384 |
- |
CSTF3 |
NNNCATTTAGAGTACTTGCA |
NGG |
2 |
0.3025 |
Tier II |
| 8 |
NC_000003.12 |
3 |
22005864 |
+ |
ZNF385D |
NNNGCTTTAGAGTACTTAAA |
NGG |
2 |
0.2967 |
Tier II |
| 9 |
NC_000014.9 |
14 |
62836486 |
+ |
KCNH5 |
NNNGCTTCAGAGTACTTGCA |
NAG |
2 |
0.2593 |
Tier II |
| 10 |
NC_000017.11 |
17 |
72814164 |
- |
SLC39A11 |
NNNGCTTTTGAGTACTTGAA |
NGG |
2 |
0.2571 |
Tier II |
| 11 |
NC_000002.12 |
2 |
111143152 |
- |
BCL2L11 |
NNNGCTTTAGATTACTTTCA |
NGG |
2 |
0.2564 |
Tier II |
| 12 |
NC_000006.12 |
6 |
42789029 |
+ |
BICRAL |
NNNGCTTGAGAGTAGTTGCA |
NGG |
2 |
0.0367 |
Tier II |
| 13 |
NC_000010.11 |
10 |
12235841 |
- |
CDC123 |
NNNGCTTTAGAGTACTTACA |
NTG |
2 |
0.027 |
Tier II |
| 14 |
NC_000010.11 |
10 |
90766961 |
- |
HTR7 |
NNNGCTTTAGAGTACCTGCA |
NTG |
2 |
0.026 |
Tier II |
| 15 |
NC_000013.11 |
13 |
94000042 |
+ |
GPC6 |
NNNGCTTTAGAGTACTTGCT |
NTG |
2 |
0.0234 |
Tier II |
| 16 |
NC_000003.12 |
3 |
185778577 |
- |
IGF2BP2 |
NNNGCTGTAGAGTACTTGCA |
NGT |
2 |
0.0095 |
Tier II |
| 17 |
NC_000020.11 |
20 |
23371267 |
+ |
GZF1 |
NNNGCTTTAGAGTACTTGAA |
NGT |
2 |
0.0069 |
Tier II |
| 18 |
NC_000016.10 |
16 |
2634519 |
- |
PDPK2P |
NNNGCTTTAGAGTACTTGCA |
NGG |
0 |
1.0 |
Tier III |
| 19 |
NC_000003.12 |
3 |
127888531 |
+ |
LOC107986129 |
NNNGCTTGAAAGTACTTGCA |
NGG |
2 |
0.6844 |
Tier III |
| 20 |
NC_000005.10 |
5 |
5272973 |
+ |
LOC101929200 |
NNNACTTTGGAGTACTTGCA |
NGG |
2 |
0.6 |
Tier III |
| 21 |
NC_000007.14 |
7 |
149563108 |
+ |
ZNF767P |
NNNGCTCTAGAGTACTTACA |
NGG |
2 |
0.476 |
Tier III |
| 22 |
NC_000006.12 |
6 |
42789029 |
+ |
LOC401261 |
NNNGCTTGAGAGTAGTTGCA |
NGG |
2 |
0.0367 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
24101626 |
- |
Pdpk1 |
NNNGCTTTAGAGTACTTGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000077.6 |
11 |
20240031 |
+ |
Cep68 |
NNNACTTTAGAGTACTTGCT |
NGG |
2 |
0.54 |
Tier I |
| 3 |
NC_000079.6 |
13 |
97161234 |
- |
Gfm2 |
NNNGCTTTAAAGTACTTTCA |
NGG |
2 |
0.6222 |
Tier II |
| 4 |
NC_000077.6 |
11 |
33309503 |
- |
Ranbp17 |
NNNCCTTTAGAATACTTGCA |
NGG |
2 |
0.4941 |
Tier II |
| 5 |
NC_000068.7 |
2 |
38271758 |
- |
Dennd1a |
NNNGATGTAGAGTACTTGCA |
NGG |
2 |
0.3361 |
Tier II |
| 6 |
NC_000068.7 |
2 |
115921169 |
- |
Meis2 |
NNNGCTTTAGATTTCTTGCA |
NGG |
2 |
0.2051 |
Tier II |
| 7 |
NC_000083.6 |
17 |
86308480 |
- |
Prkce |
NNNGCTGTAGAGTACTTGCC |
NGG |
2 |
0.1337 |
Tier II |
| 8 |
NC_000070.6 |
4 |
65943024 |
- |
Astn2 |
NNNGCTTCAGAGTACTTGCA |
NGT |
2 |
0.0161 |
Tier II |
| 9 |
NC_000071.6 |
5 |
36249144 |
- |
Sorcs2 |
NNNGCTTTAGAGTACTTGCA |
NCA |
2 |
0.0 |
Tier II |
| 10 |
NC_000069.6 |
3 |
103538352 |
+ |
Gm34049 |
NNNGCTCTAGAGTTCTTGCA |
NGG |
2 |
0.3667 |
Tier III |
| 11 |
NC_000071.6 |
5 |
111930179 |
- |
Gm36794 |
NNNGCTTAAGAGTACTTGCA |
NCG |
2 |
0.0857 |
Tier III |
| 12 |
NC_000068.7 |
2 |
147259960 |
- |
Nkx2-2os |
NNNGCTTTAGAGTATTTGCA |
NAG |
2 |
0.0173 |
Tier III |
Other clones with same target sequence:
(none)
