Construct: sgRNA BRDN0001145445

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGGGCTAATGCACAGAACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYO3B (140469)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75569

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 170401490 - MYO3B NNNGCTAATGCACAGAACAG NGG 0 1.0 Tier I
2 NC_000010.11 10 88965610 + ACTA2 NNNGCCAATGCACAGAACAG NGG 1 0.9091 Tier II
3 NC_000010.11 10 88965610 + FAS NNNGCCAATGCACAGAACAG NGG 1 0.9091 Tier II
4 NC_000013.11 13 113127889 + F10 NNNGCCAAAGCACAGAACAG NGG 2 0.8442 Tier II
5 NC_000008.11 8 36932133 - KCNU1 NNNGCTAATGCACAGAACAT NGG 1 0.7 Tier II
6 NC_000002.12 2 127703353 - WDR33 NNNGCCAATGCACAGAAAAG NGG 2 0.4895 Tier II
7 NC_000002.12 2 127703353 - SFT2D3 NNNGCCAATGCACAGAAAAG NGG 2 0.4895 Tier II
8 NC_000004.12 4 153257533 - TRIM2 NNNGTTCATGCACAGAACAG NGG 2 0.2784 Tier II
9 NC_000015.10 15 89270237 - FANCI NNNTGTAATGCACAGAACAG NGG 2 0.2182 Tier II
10 NC_000011.10 11 68924747 - IGHMBP2 NNNTCTAATCCACAGAACAG NGG 2 0.1455 Tier II
11 NC_000013.11 13 77010873 + FBXL3 NNNTCTAATGTACAGAACAG NGG 2 0.1119 Tier II
12 NC_000012.12 12 41434284 - PDZRN4 NNNGCGAATGCAGAGAACAG NGG 2 0.0779 Tier II
13 NC_000010.11 10 113091889 - TCF7L2 NNNGCTAATGCAGAGAAAAG NGG 2 0.0734 Tier II
14 NC_000019.10 19 18474772 + ELL NNNGCTACTGCAGAGAACAG NGG 2 0.0584 Tier II
15 NC_000006.12 6 150230822 + PPP1R14C NNNGGTAATGCACAGAACAG NGA 2 0.0417 Tier II
16 NC_000001.11 1 205012112 - NFASC NNNGCTAGTGCACAGCACAG NGG 2 0.0 Tier II
17 NC_000013.11 13 27853359 - PLUT NNNGCTGATGCACAGAACAG NCG 2 0.0756 Tier III
18 NC_000018.10 18 35464124 + ZNF24TR NNNTCTAATGCACAGACCAG NGG 2 0.0642 Tier III
19 NC_000006.12 6 134442785 - LINC01010 NNNGCTAATGCAAAGAAGAG NGG 2 0.0513 Tier III
20 NC_000006.12 6 134442785 - CT69 NNNGCTAATGCAAAGAAGAG NGG 2 0.0513 Tier III
21 NC_000010.11 10 36055332 - LOC107984222 NNNGCTAATGCCCAGGACAG NGG 2 0.0506 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 40629452 - Slc9a4 NNNGCTACTGCATAGAACAG NGG 2 0.3 Tier I
2 NC_000083.6 17 37009981 + Zfp57 NNNGCTAAGGCACGGAACAG NGG 2 0.2889 Tier I
3 NC_000077.6 11 105412999 + Marchf10 NNNGCCAGTGCACAGAACAG NGG 2 0.6667 Tier II
4 NC_000070.6 4 99729654 - Alg6 NNNGGTAATGCACAGAACAA NGG 2 0.5625 Tier II
5 NC_000067.6 1 186669972 + Tgfb2 NNNGCTAAAGCACAGAAAAG NGG 2 0.5 Tier II
6 NC_000084.6 18 35599094 - Paip2 NNNGAAAATGCACAGAACAG NGG 2 0.4952 Tier II
7 NC_000077.6 11 70395616 + Pelp1 NNNCCCAATGCACAGAACAG NGG 2 0.4813 Tier II
8 NC_000071.6 5 136830309 + Col26a1 NNNGCTCATACACAGAACAG NGG 2 0.4083 Tier II
9 NC_000067.6 1 171154752 - Mpz NNNGCCAATGCACAGAACGG NGG 2 0.3409 Tier II
10 NC_000071.6 5 23945032 - Fam126a NNNGCTATTCCACAGAACAG NGG 2 0.1714 Tier II
11 NC_000077.6 11 57151476 + Gria1 NNNGCTCATGCTCAGAACAG NGG 2 0.1458 Tier II
12 NC_000072.6 6 97436539 - Frmd4b NNNGCTAATGCCCAGAAAAG NGG 2 0.1417 Tier II
13 NC_000070.6 4 111277452 - Agbl4 NNNGCTACTGCACAGAACAG NAG 2 0.1111 Tier II
14 NC_000080.6 14 29423245 + Cacna2d3 NNNGCTAATGCAAAGAACAG NAG 2 0.0997 Tier II
15 NC_000078.6 12 30245086 + Sntg2 NNNGCTAATGCAAAGAAGAG NGG 2 0.0513 Tier II
16 NC_000084.6 18 38787330 - Arhgap26 NNNGCAAATGCACAGAACAG NGT 2 0.014 Tier II
17 NC_000078.6 12 117260294 + Ptprn2 NNNGCTAGTGCACAGAACAG NGT 2 0.0118 Tier II
18 NC_000071.6 5 100862499 - Gpat3 NNNGCTAAGGCACAGCACAG NGG 2 0.0 Tier II
19 NC_000076.6 10 110203148 - Nav3 NNNGCTAATGCACAGAACAG NCC 2 0.0 Tier II
20 NC_000071.6 5 92689250 + Shroom3 NNNGCTAATGTACAGCACAG NGG 2 0.0 Tier II
21 NC_000071.6 5 136830309 + Gm30654 NNNGCTCATACACAGAACAG NGG 2 0.4083 Tier III
22 NC_000074.6 8 39685221 - Gm36741 NNNACTAATGCACAGAACAG NAG 2 0.2333 Tier III
23 NC_000072.6 6 53224741 - Gm4872 NNNGCTAATGCACATAACTG NGG 2 0.0769 Tier III
24 NC_000067.6 1 51514528 - Gm17767 NNNGCTAATGCTGAGAACAG NGG 2 0.0455 Tier III
25 NC_000078.6 12 117260294 + Gm5441 NNNGCTAGTGCACAGAACAG NGT 2 0.0118 Tier III
26 NC_000076.6 10 110203148 - LOC115487471 NNNGCTAATGCACAGAACAG NCC 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)