Construct: sgRNA BRDN0001145492
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGTATTTCTACACGAACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PHKA1 (5255)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77840
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
72618840 |
+ |
PHKA1 |
NNNTATTTCTACACGAACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
154445155 |
+ |
IL6R |
NNNTGTTTCTACACGAATAG |
NGG |
2 |
0.4629 |
Tier II |
| 3 |
NC_000003.12 |
3 |
25771839 |
- |
NGLY1 |
NNNTATTTCTACACCAACAG |
NGA |
2 |
0.0189 |
Tier II |
| 4 |
NC_000011.10 |
11 |
3002259 |
+ |
CARS1 |
NNNTATTTCTACAGGAATAG |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000003.12 |
3 |
173223588 |
- |
LOC105374224 |
NNNTATTTCTACTAGAACAG |
NGG |
2 |
0.105 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
30576268 |
- |
Kmt2b |
NNNTATTTCTACAAGAACAG |
NGG |
1 |
0.35 |
Tier II |
| 2 |
NC_000070.6 |
4 |
9389754 |
- |
Clvs1 |
NNNTAATTCTTCACGAACAG |
NGG |
2 |
0.2667 |
Tier II |
| 3 |
NC_000072.6 |
6 |
61669005 |
- |
Ccser1 |
NNNAATTTCTACACGAACTG |
NGG |
2 |
0.2564 |
Tier II |
| 4 |
NC_000085.6 |
19 |
48706823 |
- |
Sorcs3 |
NNNCATTTCTACAAGAACAG |
NGG |
2 |
0.2265 |
Tier II |
| 5 |
NC_000080.6 |
14 |
64262795 |
+ |
Msra |
NNNTATTACTACACCAACAG |
NGG |
2 |
0.2182 |
Tier II |
| 6 |
NC_000080.6 |
14 |
121950948 |
+ |
Ubac2 |
NNNTATGTCTACACGATCAG |
NGG |
2 |
0.0784 |
Tier II |
| 7 |
NC_000081.6 |
15 |
97255081 |
+ |
Pced1b |
NNNTATTTCTACACCACCAG |
NGG |
2 |
0.0481 |
Tier II |
| 8 |
NC_000084.6 |
18 |
86499332 |
+ |
Neto1 |
NNNTATTTCTACACAAACAG |
NGC |
2 |
0.0209 |
Tier II |
| 9 |
NC_000080.6 |
14 |
9622855 |
- |
Fhit |
NNNTATTGCTACAGGAACAG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000082.6 |
16 |
16825718 |
+ |
Spag6l |
NNNTATTTCTACAGGTACAG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000074.6 |
8 |
32249531 |
- |
Nrg1 |
NNNTATTTCTTCAGGAACAG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000080.6 |
14 |
64262795 |
+ |
Gm41176 |
NNNTATTACTACACCAACAG |
NGG |
2 |
0.2182 |
Tier III |
Other clones with same target sequence:
(none)
