Construct: sgRNA BRDN0001145588

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGGAAGGAAACATGGAACTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKCA (5578)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75926

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 66689030 + PRKCA NNNAAGGAAACATGGAACTC NGG 0 1.0 Tier I
2 NC_000004.12 4 20716178 + PACRGL NNNAAGGATCCATGGAACTC NGG 2 0.2 Tier I
3 NC_000001.11 1 26342817 - CRYBG2 NNNAAGGAGACATGGAACTC NGA 2 0.0463 Tier I
4 NC_000012.12 12 76439627 - OSBPL8 NNNAAGAAAACATGAAACTC NGG 2 0.9412 Tier II
5 NC_000005.10 5 159875430 + ADRA1B NNNAAGGAAAAATGAAACTC NGG 2 0.7059 Tier II
6 NC_000007.14 7 107607614 - BCAP29 NNNAAGAAAACATGGAATTC NGG 2 0.6429 Tier II
7 NC_000007.14 7 107607614 - DUS4L-BCAP29 NNNAAGAAAACATGGAATTC NGG 2 0.6429 Tier II
8 NC_000006.12 6 161833998 + PRKN NNNTAGAAAACATGGAACTC NGG 2 0.6364 Tier II
9 NC_000016.10 16 265520 + FAM234A NNNAAGGAGTCATGGAACTC NGG 2 0.5882 Tier II
10 NC_000009.12 9 20822358 + FOCAD NNNAAGGAAACAAGGAATTC NGG 2 0.4451 Tier II
11 NC_000002.12 2 164708815 - COBLL1 NNNAGGGAAACATGGAACTA NGG 2 0.36 Tier II
12 NC_000002.12 2 115422273 + DPP10 NNNAAGGCAACATAGAACTC NGG 2 0.3214 Tier II
13 NC_000013.11 13 77077001 + MYCBP2 NNNAATGCAACATGGAACTC NGG 2 0.2857 Tier II
14 NC_000004.12 4 44645703 + YIPF7 NNNAAAGAAACATTGAACTC NGG 2 0.2667 Tier II
15 NC_000015.10 15 28219015 + HERC2 NNNAAGAAAAGATGGAACTC NGG 2 0.25 Tier II
16 NC_000023.11 X 46518152 + ZNF674 NNNAGGGAAACATGGAACTT NGG 2 0.216 Tier II
17 NC_000009.12 9 37020289 + PAX5 NNNAATGAAACATTGAACTC NGG 2 0.1778 Tier II
18 NC_000002.12 2 229480863 + DNER NNNCAGGAAACATGGAACTA NGG 2 0.1765 Tier II
19 NC_000020.11 20 20130422 - CFAP61 NNNCAGGAAACATGGAACTA NGG 2 0.1765 Tier II
20 NC_000005.10 5 130106827 + CHSY3 NNNAAGGAAACATGGAAATT NGG 2 0.1615 Tier II
21 NC_000005.10 5 159977205 + ADRA1B NNNAAGGACACATGGAACCC NGG 2 0.1576 Tier II
22 NC_000023.11 X 17127094 - REPS2 NNNAAGGACACATGGAACCC NGG 2 0.1576 Tier II
23 NC_000005.10 5 663387 - TPPP NNNAAGGAAAAATGGACCTC NGG 2 0.1324 Tier II
24 NC_000005.10 5 663387 - CEP72 NNNAAGGAAAAATGGACCTC NGG 2 0.1324 Tier II
25 NC_000012.12 12 56614970 - BAZ2A NNNAAGGAAAAATGGAGCTC NGG 2 0.1324 Tier II
26 NC_000012.12 12 125039189 + BRI3BP NNNAAGGAATCATGGAAGTC NGG 2 0.1176 Tier II
27 NC_000001.11 1 166082166 - FAM78B NNNAAGGAAACATGGACATC NGG 2 0.095 Tier II
28 NC_000019.10 19 42220933 + ZNF526 NNNAAGGAAAAATGGAACTC NCG 2 0.0804 Tier II
29 NC_000003.12 3 4435921 + SUMF1 NNNAAGGAAACATGGAACTT NAG 2 0.0778 Tier II
30 NC_000020.11 20 3606599 - ATRN NNNAAGGAAGCATGGAAGTC NGG 2 0.0741 Tier II
31 NC_000021.9 21 45923448 - PCBP3 NNNAAGGAAAGATGGAACTC NAG 2 0.0648 Tier II
32 NC_000014.9 14 76782753 - VASH1 NNNAAGGAAACTTGGGACTC NGG 2 0.0641 Tier II
33 NC_000002.12 2 51013223 + NRXN1 NNNAAGGAAACATGAAACTG NGG 2 0.0554 Tier II
34 NC_000003.12 3 171401147 - TNIK NNNAAGGAAATATGGAGCTC NGG 2 0.0543 Tier II
35 NC_000009.12 9 33254368 + BAG1 NNNAAGGAAACATAGAACTC NGA 2 0.0521 Tier II
36 NC_000008.11 8 32241022 + NRG1 NNNAAGGAAAGATGGGACTC NGG 2 0.0481 Tier II
37 NC_000004.12 4 109064727 - COL25A1 NNNAAGGAAACATGGAGCTC NAG 2 0.0458 Tier II
38 NC_000020.11 20 2948089 + PTPRA NNNAAGGAAACATGGAATTC NGA 2 0.0446 Tier II
39 NC_000018.10 18 37466386 + CELF4 NNNAAGGAAAAATGGAACTG NGG 2 0.0441 Tier II
40 NC_000014.9 14 93989914 - CCDC197 NNNAAGGAAACAGGGATCTC NGG 2 0.0348 Tier II
41 NC_000013.11 13 112394117 - SPACA7 NNNAAGGACACATGGAACTG NGG 2 0.0336 Tier II
42 NC_000005.10 5 78084122 + AP3B1 NNNAAGGAAACATGGAAATC NGC 2 0.012 Tier II
43 NC_000003.12 3 134769011 + CEP63 NNNAAGGAAACAGGGAACTC NGC 2 0.0058 Tier II
44 NC_000008.11 8 62680187 + NKAIN3 NNNAAGGAAACTTGGAACTC NGT 2 0.0054 Tier II
45 NC_000005.10 5 77208641 + PDE8B NNNAAGGAAACATGGATCTC NGC 2 0.003 Tier II
46 NC_000003.12 3 4181616 - SUMF1 NNNAAGGAAACATGGAACTC NTT 2 0.0 Tier II
47 NC_000023.11 X 120056812 - RHOXF1-AS1 NNNAAGGAAAAATGGAACTC NGG 1 0.75 Tier III
48 NC_000001.11 1 87157234 - LINC01140 NNNAAGGAAAAATAGAACTC NGG 2 0.5625 Tier III
49 NC_000004.12 4 187332322 - LOC339975 NNNAGGGAAACATGGAAATC NGG 2 0.3877 Tier III
50 NC_000014.9 14 79889095 + LOC105370582 NNNAAGGAAACATGGAACAC NGG 1 0.2857 Tier III
51 NC_000013.11 13 77077001 + MYCBP2-AS1 NNNAATGCAACATGGAACTC NGG 2 0.2857 Tier III
52 NC_000008.11 8 84163592 - LOC101929943 NNNAAGGAAAGATGGAACTC NGG 1 0.25 Tier III
53 NC_000018.10 18 71786143 + LOC105376873 NNNAAGGAAATAAGGAACTC NGG 2 0.213 Tier III
54 NC_000002.12 2 138052815 - LOC105373636 NNNAAGGAAACATGGAAATT NGG 2 0.1615 Tier III
55 NC_000012.12 12 119263074 + LOC124903032 NNNAAGGACACATGGAACCC NGG 2 0.1576 Tier III
56 NC_000006.12 6 74467207 - LOC101928516 NNNAAGGAAGCATGGAACCC NGG 2 0.1533 Tier III
57 NC_000005.10 5 79334280 + LOC102724530 NNNAAGGTAACATGGAACAC NGG 2 0.1224 Tier III
58 NC_000001.11 1 166082166 - FAM78B-AS1 NNNAAGGAAACATGGACATC NGG 2 0.095 Tier III
59 NC_000007.14 7 26077530 + LOC105375199 NNNAAGGAAAGATGGAACAC NGG 2 0.0714 Tier III
60 NC_000014.9 14 76782753 - VASH1-AS1 NNNAAGGAAACTTGGGACTC NGG 2 0.0641 Tier III
61 NC_000010.11 10 14488117 - LOC124902380 NNNAAGAAAACATGGAACTG NGG 2 0.0588 Tier III
62 NC_000018.10 18 37466386 + SNORA111 NNNAAGGAAAAATGGAACTG NGG 2 0.0441 Tier III
63 NC_000014.9 14 93989914 - LOC107984663 NNNAAGGAAACAGGGATCTC NGG 2 0.0348 Tier III
64 NC_000013.11 13 110956402 + LOC105370364 NNNAAGGACACATGGAACTG NGG 2 0.0336 Tier III
65 NC_000013.11 13 112394117 - LOC105370372 NNNAAGGACACATGGAACTG NGG 2 0.0336 Tier III
66 NC_000005.10 5 61702210 + LINC03122 NNNAAGGAAACATGGAAGGC NGG 2 0.0333 Tier III
67 NC_000001.11 1 110295228 + RBM15-AS1 NNNAAGAAAACATGGAACTC NGT 2 0.0161 Tier III
68 NC_000004.12 4 137669762 - LOC101927414 NNNAAGGAAACATGAAACTC NGT 2 0.0152 Tier III
69 NC_000004.12 4 137669762 - LOC107986314 NNNAAGGAAACATGAAACTC NGT 2 0.0152 Tier III
70 NC_000005.10 5 153975450 + LOC107983980 NNNAAGGAAACATGGCACTG NGG 2 0.0 Tier III
71 NC_000014.9 14 41017520 - LINC02315 NNNAAGGAAACATGGTCCTC NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 108012645 - Prkca NNNAAGGCAACATGGAACTC NGG 1 0.4286 Tier I
2 NC_000083.6 17 35130852 - Apom NNNAAGGAAACATGGAACTC NGA 1 0.0694 Tier I
3 NC_000082.6 16 59851859 - Epha6 NNNAAGGAAAAATGAAACTC NGG 2 0.7059 Tier II
4 NC_000072.6 6 63435122 + Grid2 NNNAATGAAACATGGAACTC NGG 1 0.6667 Tier II
5 NC_000071.6 5 139389630 + 3110082I17Rik NNNAAGGGAAAATGGAACTC NGG 2 0.55 Tier II
6 NC_000071.6 5 139389630 + Gpr146 NNNAAGGGAAAATGGAACTC NGG 2 0.55 Tier II
7 NC_000074.6 8 120366996 - Gse1 NNNAAGGAAACATAGAATTC NGG 2 0.4821 Tier II
8 NC_000083.6 17 47506953 - Ccnd3 NNNAAGGACAAATGGAACTC NGG 2 0.4286 Tier II
9 NC_000082.6 16 10697136 - Clec16a NNNAGGGACACATGGAACTC NGG 2 0.4114 Tier II
10 NC_000069.6 3 145415012 + Col24a1 NNNAAAGAAACATGGAACTT NGG 2 0.3 Tier II
11 NC_000078.6 12 37132244 + Meox2 NNNAAGGAAAATTGGAACTC NGG 2 0.25 Tier II
12 NC_000073.6 7 120106739 + Tmem159 NNNCAGGAAACAAGGAACTC NGG 2 0.2443 Tier II
13 NC_000067.6 1 129837720 + Thsd7b NNNAAGGAAACACTGAACTC NGG 2 0.2105 Tier II
14 NC_000073.6 7 114110729 - Rras2 NNNAAGCAAACATTGAACTC NGG 2 0.1833 Tier II
15 NC_000068.7 2 20083453 + Etl4 NNNAAGGAAACCAGGAACTC NGG 2 0.1822 Tier II
16 NC_000084.6 18 38296370 + Rnf14 NNNAGGGAAACATGGAACGC NGG 2 0.18 Tier II
17 NC_000070.6 4 150552080 + Rere NNNAAGGAAACTTGGAAATC NGG 2 0.1795 Tier II
18 NC_000071.6 5 52080726 + Ppargc1a NNNAAGGAAAGAAGGAACTC NGG 2 0.1731 Tier II
19 NC_000067.6 1 79760220 - Wdfy1 NNNGAGGAAACATGGAACCC NGG 2 0.1724 Tier II
20 NC_000086.7 X 136833619 + Plp1 NNNAAGGAAACATGGAAATT NGG 2 0.1615 Tier II
21 NC_000073.6 7 31130440 - Gramd1a NNNAAGGACACATGGAACCC NGG 2 0.1576 Tier II
22 NC_000085.6 19 58101471 - Atrnl1 NNNAAGGACACATGGAACCC NGG 2 0.1576 Tier II
23 NC_000068.7 2 146033527 + Cfap61 NNNACGGAAATATGGAACTC NGG 2 0.1538 Tier II
24 NC_000072.6 6 18107050 + Asz1 NNNAAGTAAACATTGAACTC NGG 2 0.1524 Tier II
25 NC_000068.7 2 180974631 + Arfgap1 NNNAAGGCAACTTGGAACTC NGG 2 0.1429 Tier II
26 NC_000079.6 13 112692219 + Slc38a9 NNNAAGGAAACATGAATCTC NGG 2 0.1255 Tier II
27 NC_000074.6 8 108314766 + Zfhx3 NNNAAGGAAAGATGGAACTA NGG 2 0.125 Tier II
28 NC_000068.7 2 125091958 - Myef2 NNNAAGGCAACATTGAACTC NGG 2 0.1143 Tier II
29 NC_000069.6 3 79928855 + Gask1b NNNAGGGAAACATGGAAGTC NGG 2 0.096 Tier II
30 NC_000069.6 3 108101440 - Gnat2 NNNAAGGAAACAGGGAACCC NGG 2 0.072 Tier II
31 NC_000071.6 5 115051155 + Sppl3 NNNAAGGAAAGATGGAACGC NGG 2 0.0625 Tier II
32 NC_000073.6 7 46939496 + Uevld NNNAAGGAAACATGTAACTT NGG 2 0.0429 Tier II
33 NC_000070.6 4 124875462 - Maneal NNNAAGGAAACATTGAACTG NGG 2 0.0157 Tier II
34 NC_000069.6 3 81183716 - Pdgfc NNNAAGGAAACATGCAACTC NTG 2 0.0106 Tier II
35 NC_000085.6 19 56427000 - Casp7 NNNAAGGAAACATGGGACTC NTG 2 0.0075 Tier II
36 NC_000076.6 10 28367460 + Ptprk NNNAAGGAAACATGGAAGTC NGC 2 0.003 Tier II
37 NC_000079.6 13 19496838 - 9330199G10Rik NNNAAGGAAACATGAAACTC NGG 1 0.9412 Tier III
38 NC_000084.6 18 4359644 - 4833419F23Rik NNNAAGGAAAGATAGAACTC NGG 2 0.1875 Tier III
39 NC_000078.6 12 114494967 + Igh NNNAAGGAAACATAGACCTC NGG 2 0.1324 Tier III
40 NC_000078.6 12 114546315 + Igh NNNAAGGAAACATAGACCTC NGG 2 0.1324 Tier III
41 NC_000076.6 10 97710085 - Gm10754 NNNAAGGAAACATGGAATTC NGA 2 0.0446 Tier III
42 NC_000076.6 10 84056054 + Gm34184 NNNAAGGAAACATGGAACAC NGA 2 0.0198 Tier III
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Other clones with same target sequence:

(none)