Construct: sgRNA BRDN0001145662

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CGAAGTCTTTCTTAGATGGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80233

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000014.9 14 50916831 - PYGL NNNAGTCTTTCATAGAAGGT NGG 2 0.4267 Tier II
2 NC_000002.12 2 154002009 + GALNT13 NNNAGTCTTTCTTGGAAGGT NGG 2 0.2489 Tier II
3 NC_000003.12 3 52730732 + NEK4 NNNTTTCTTTCTTAGATGGT NGG 2 0.1909 Tier II
4 NC_000004.12 4 146423947 - SLC10A7 NNNGGTCTTTCTTACATGGT NGG 2 0.1705 Tier II
5 NC_000015.10 15 27235374 + GABRG3 NNNAGGCTTTCTTAGATGGT NAG 2 0.1481 Tier II
6 NC_000014.9 14 37443579 - MIPOL1 NNNTGTCTTTCTTATATGGT NGG 2 0.0909 Tier II
7 NC_000010.11 10 119815307 + INPP5F NNNATTCTTTCTTAGATGGT NAG 2 0.0778 Tier II
8 NC_000018.10 18 36894986 - KIAA1328 NNNAGTCTTTCTTAGCTGGT NTG 2 0.0 Tier II
9 NC_000001.11 1 15952889 - ZBTB17 NNNAGTCTTTCTTAGTTGGA NGG 2 0.0 Tier II
10 NC_000018.10 18 32914582 + LOC105372058 NNNAGTCATTCTTAAATGGT NGG 2 0.7529 Tier III
11 NC_000016.10 16 65529326 - LINC00922 NNNAGTCTGACTTAGATGGT NGG 2 0.5306 Tier III
12 NC_000004.12 4 79856241 - PCAT4 NNNAGTCTTTCTTGGATGGT NGG 1 0.4667 Tier III
13 NC_000007.14 7 25349641 - LOC105375195 NNNAGTCTTTTTTAGAAGGT NGG 2 0.1641 Tier III
14 NC_000010.11 10 119815307 + PHACTR2P1 NNNATTCTTTCTTAGATGGT NAG 2 0.0778 Tier III
15 NC_000010.11 10 119815307 + LOC105378513 NNNATTCTTTCTTAGATGGT NAG 2 0.0778 Tier III
16 NC_000011.10 11 45520885 + LOC105376654 NNNAGTCTTTCTGAGATGGG NGG 2 0.046 Tier III
17 NC_000001.11 1 15952889 - LOC124903855 NNNAGTCTTTCTTAGTTGGA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 94079168 + Fgd6 NNNAGTCTTACTTAGATGGT NGG 1 0.8571 Tier II
2 NC_000069.6 3 159502355 - Depdc1a NNNAGTCCTTCTTAGATGGA NGG 2 0.5625 Tier II
3 NC_000076.6 10 114439617 + Trhde NNNAGTCCTTCTTAGAAGGT NGG 2 0.5333 Tier II
4 NC_000084.6 18 57587704 - Ccdc192 NNNAGTCTTTCTCAGAAGGT NGG 2 0.4211 Tier II
5 NC_000083.6 17 86058639 - Srbd1 NNNAGTGTGTCTTAGATGGT NGG 2 0.2913 Tier II
6 NC_000079.6 13 108089101 - Ndufaf2 NNNATTCTTTCTTAGAAGGT NGG 2 0.16 Tier II
7 NC_000068.7 2 181692549 + Rgs19 NNNAGGCTTTCTGAGATGGT NGG 2 0.1491 Tier II
8 NC_000077.6 11 4944851 + Nefh NNNATTCTTTCTTAGATGCT NGG 2 0.1345 Tier II
9 NC_000071.6 5 98661771 - Cfap299 NNNAGTCTTGCTGAGATGGT NGG 2 0.1304 Tier II
10 NC_000079.6 13 100126765 + Smn1 NNNAGGCTTTCTTAGGTGGT NGG 2 0.1099 Tier II
11 NC_000079.6 13 117629562 - Hcn1 NNNAGTCTTTCTTAGAAGGG NGG 2 0.0941 Tier II
12 NC_000081.6 15 35462682 - Vps13b NNNAGTCTTTCTTAGAAGGG NGG 2 0.0941 Tier II
13 NC_000068.7 2 26471633 - Notch1 NNNATTCTTTCTGAGATGGT NGG 2 0.0783 Tier II
14 NC_000068.7 2 69382791 - Dhrs9 NNNAGTCTTTCTTAGATGGT NGA 1 0.0694 Tier II
15 NC_000076.6 10 13711842 - Aig1 NNNAGTCTTTCTTCCATGGT NGG 2 0.0584 Tier II
16 NC_000067.6 1 68614232 - Erbb4 NNNAGTCTTTCTTAGAAGGC NGG 2 0.0485 Tier II
17 NC_000086.7 X 113818256 - Dach2 NNNAGTCTTTCTTAGAAGGT NGA 2 0.037 Tier II
18 NC_000067.6 1 61890369 - Pard3b NNNAGTCTTTCTTAGATGCT NGA 2 0.0311 Tier II
19 NC_000069.6 3 145806442 - Ddah1 NNNAGACTTTCTTAGATGGT NGT 2 0.014 Tier II
20 NC_000068.7 2 5416220 + Camk1d NNNAGCCTTTCTTAGTTGGT NGG 2 0.0 Tier II
21 NC_000073.6 7 142545845 + Nctc1 NNNAGCCTTTCTTTGATGGT NGG 2 0.4848 Tier III
22 NC_000081.6 15 96494866 - Gm41392 NNNAGCCTTTCTGAGATGGT NGG 2 0.2372 Tier III
23 NC_000072.6 6 114359171 + Gm38875 NNNAGTCTTTGTTAGACGGT NGG 2 0.1765 Tier III
24 NC_000068.7 2 69382791 - Gm36393 NNNAGTCTTTCTTAGATGGT NGA 1 0.0694 Tier III
25 NC_000068.7 2 78989294 + Gm37004 NNNAGTCTGTCTTAGATGGC NGG 2 0.0563 Tier III
26 NC_000083.6 17 94814793 + Gm1976 NNNAGTCTTACTTAGATGGT NGC 2 0.019 Tier III
27 NC_000085.6 19 43640971 + Gm4311 NNNAGTCTTTATTAGCTGGT NGG 2 0.0 Tier III
28 NC_000085.6 19 43640971 + Gm34922 NNNAGTCTTTATTAGCTGGT NGG 2 0.0 Tier III
29 NC_000080.6 14 104659855 + D130009I18Rik NNNAGTCTTTCTTAGTTGCT NGG 2 0.0 Tier III
30 NC_000071.6 5 75683070 + Gm32558 NNNAGTCTTTGTTAGCTGGT NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)