Construct: sgRNA BRDN0001145693

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCTCCATCAAAAATGAAGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKCQ (5588)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75554

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 6485189 - PRKCQ NNNCCATCAAAAATGAAGCA NGG 0 1.0 Tier I
2 NC_000003.12 3 47909801 - MAP4 NNNCCATGAAAAATGAAGCA NGG 1 0.6429 Tier I
3 NC_000012.12 12 116974638 + FBXW8 NNNCCAATAAAAATGAAGCA NGG 2 0.7656 Tier II
4 NC_000012.12 12 125379831 + TMEM132B NNNCCATCATGAATGAAGCA NGG 2 0.5735 Tier II
5 NC_000017.11 17 80863962 + RPTOR NNNCTAACAAAAATGAAGCA NGG 2 0.5568 Tier II
6 NC_000011.10 11 125405424 + PKNOX2 NNNCTATTAAAAATGAAGCA NGG 2 0.5568 Tier II
7 NC_000001.11 1 59337929 - FGGY NNNCCATCAGAAATGAAGCA NGG 1 0.5556 Tier II
8 NC_000020.11 20 46724168 - SLC2A10 NNNTCATAAAAAATGAAGCA NGG 2 0.52 Tier II
9 NC_000003.12 3 68978304 + EOGT NNNCAATTAAAAATGAAGCA NGG 2 0.5 Tier II
10 NC_000002.12 2 20672029 - GDF7 NNNCTTTCAAAAATGAAGCA NGG 2 0.4545 Tier II
11 NC_000001.11 1 71955492 + NEGR1 NNNCCACCAAAAGTGAAGCA NGG 2 0.4484 Tier II
12 NC_000004.12 4 81141890 + PRKG2 NNNCCATCAGAGATGAAGCA NGG 2 0.4012 Tier II
13 NC_000006.12 6 45006378 - SUPT3H NNNCCATTAAAAATGAAGAA NGG 2 0.375 Tier II
14 NC_000019.10 19 41399525 - BCKDHA NNNCCACCAAAAATGAAGTA NGG 2 0.3173 Tier II
15 NC_000005.10 5 10721670 + DAP NNNCCCTCAAGAATGAAGCA NGG 2 0.2955 Tier II
16 NC_000012.12 12 108246713 + WSCD2 NNNCCATGAAAAATGAAGAA NGG 2 0.2755 Tier II
17 NC_000023.11 X 33309904 + DMD NNNCCATCAAAAATGAAGCA NAG 1 0.2593 Tier II
18 NC_000013.11 13 33382101 + STARD13 NNNCCATCATAAACGAAGCA NGG 2 0.2521 Tier II
19 NC_000003.12 3 111751345 + PHLDB2 NNNCCATTAAAAATGAAGCA NAG 2 0.2269 Tier II
20 NC_000003.12 3 49533457 - DAG1 NNNCCATCAAAAATGAAGCG NAG 2 0.1983 Tier II
21 NC_000023.11 X 108123772 + ATG4A NNNCCATCCCAAATGAAGCA NGG 2 0.1905 Tier II
22 NC_000003.12 3 10853798 - SLC6A11 NNNCCATCAAACATGAATCA NGG 2 0.1754 Tier II
23 NC_000008.11 8 27830436 + PBK NNNCTATCAAAAATGAAGCA NAG 2 0.165 Tier II
24 NC_000016.10 16 71032772 - HYDIN NNNGCATCAAAAATGAAGCA NAG 2 0.1296 Tier II
25 NC_000020.11 20 42672545 + PTPRT NNNCCATCTAAACTGAAGCA NGG 2 0.1263 Tier II
26 NC_000006.12 6 131690386 + ENPP3 NNNCCATCAACATTGAAGCA NGG 2 0.12 Tier II
27 NC_000003.12 3 114912195 + ZBTB20 NNNCCTTCAAAAATGAAGGA NGG 2 0.0893 Tier II
28 NC_000008.11 8 1481212 + DLGAP2 NNNCCATCAAAAATGAAAGA NGG 2 0.0865 Tier II
29 NC_000008.11 8 76685312 - ZFHX4 NNNCCATAAAAAATGAAGGA NGG 2 0.0813 Tier II
30 NC_000005.10 5 93992189 + ARB2A NNNCCTTCAAAAATGAAGCA NGA 2 0.0496 Tier II
31 NC_000017.11 17 9163715 + NTN1 NNNCCAACAAAAATGAAGCA NTG 2 0.0341 Tier II
32 NC_000002.12 2 50258075 - NRXN1 NNNCCATCAACAATGAAGCA NGA 2 0.0278 Tier II
33 NC_000013.11 13 25733449 - ATP8A2 NNNCCATGAAAAATGAAGCA NGC 2 0.0143 Tier II
34 NC_000008.11 8 10195810 - MSRA NNNCCATCAAAAATGGAGCA NGA 2 0.0134 Tier II
35 NC_000022.11 22 32061810 - SLC5A1 NNNCCATCCAAAATGAAGCA NGC 2 0.0127 Tier II
36 NC_000008.11 8 96812378 + CPQ NNNCCTTCAAAAATGAAGCA NGT 2 0.0115 Tier II
37 NC_000008.11 8 80044581 + TPD52 NNNCCCTCAAAAATGAAGCA NGC 2 0.0101 Tier II
38 NC_000008.11 8 80044581 + TPD52-MRPS28 NNNCCCTCAAAAATGAAGCA NGC 2 0.0101 Tier II
39 NC_000007.14 7 39182921 + POU6F2 NNNCCAGCAAAAATGCAGCA NGG 2 0.0 Tier II
40 NC_000012.12 12 130606376 + RIMBP2 NNNCCATAAAAAAGGAAGCA NGG 2 0.0 Tier II
41 NC_000002.12 2 205291734 + PARD3B NNNCCATCAAAAAGGAAGCA NAG 2 0.0 Tier II
42 NC_000018.10 18 76886082 - ZNF236 NNNCCATCAAAAAGGAAGCA NGC 2 0.0 Tier II
43 NC_000002.12 2 224712880 - CCDC195 NNNCCATCAAAAAGGAAGGA NGG 2 0.0 Tier II
44 NC_000007.14 7 36433734 - ANLN NNNCCATCAAAAATGAAGCA NAA 2 0.0 Tier II
45 NC_000003.12 3 67562045 - SUCLG2 NNNCCATCAAAAATGCAGAA NGG 2 0.0 Tier II
46 NC_000011.10 11 19511548 + NAV2 NNNCCATCAAAATTGTAGCA NGG 2 0.0 Tier II
47 NC_000007.14 7 86854837 + GRM3 NNNCCATCAAAGAGGAAGCA NGG 2 0.0 Tier II
48 NC_000010.11 10 122217736 - TACC2 NNNCTATCAAAAAGGAAGCA NGG 2 0.0 Tier II
49 NC_000010.11 10 37409986 + LOC107984223 NNNCTAACAAAAATGAAGCA NGG 2 0.5568 Tier III
50 NC_000012.12 12 10727913 + LOC112268104 NNNCCCTCAAAAATAAAGCA NGG 2 0.4278 Tier III
51 NC_000008.11 8 80225460 - LOC105375920 NNNCCATCCAAAATGAAACA NGG 2 0.3956 Tier III
52 NC_000006.12 6 93740861 + TSG1 NNNCAATCAAAAATGAACCA NGG 2 0.2721 Tier III
53 NC_000002.12 2 21073048 + LOC124905593 NNNCCATCAGCAATGAAGCA NGG 2 0.2222 Tier III
54 NC_000011.10 11 105466203 + LOC105369468 NNNCCATAAAAAATGAAGCA NAG 2 0.1685 Tier III
55 NC_000014.9 14 46600934 - LOC105370481 NNNCCCTCAAATATGAAGCA NGG 2 0.1515 Tier III
56 NC_000006.12 6 127610619 + LOC105377996 NNNCCATGAAAACTGAAGCA NGG 2 0.1353 Tier III
57 NC_000001.11 1 146678154 + HYDIN2 NNNGCATCAAAAATGAAGCA NAG 2 0.1296 Tier III
58 NC_000005.10 5 124331013 - LINC01170 NNNCCATCAACAATGAGGCA NGG 2 0.0706 Tier III
59 NC_000013.11 13 62888621 + LOC105370234 NNNCTATCAAAAATGAAGCA NGA 2 0.0442 Tier III
60 NC_000007.14 7 94323465 + LOC107986821 NNNCCATCAAAAATTAAGCA NGA 2 0.0099 Tier III
61 NC_000007.14 7 94323465 + LOC112267858 NNNCCATCAAAAATTAAGCA NGA 2 0.0099 Tier III
62 NC_000018.10 18 76886082 - LOC100421894 NNNCCATCAAAAAGGAAGCA NGC 2 0.0 Tier III
63 NC_000002.12 2 224712880 - LOC105373910 NNNCCATCAAAAAGGAAGGA NGG 2 0.0 Tier III
64 NC_000011.10 11 19511548 + NAV2-AS4 NNNCCATCAAAATTGTAGCA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 21827195 - Gpr158 NNNCCTTCAAAAACGAAGCA NGG 2 0.2041 Tier I
2 NC_000071.6 5 73023894 - Fryl NNNCCATCAAGAATGAAGCA NAG 2 0.1685 Tier I
3 NC_000072.6 6 63507062 - Grid2 NNNCCACCAAAAATAAAGCA NGG 2 0.6471 Tier II
4 NC_000068.7 2 26277555 + Ccdc187 NNNCCAACAAAAATGAAGCT NGG 2 0.525 Tier II
5 NC_000084.6 18 82668006 + Zfp236 NNNACATCTAAAATGAAGCA NGG 2 0.5053 Tier II
6 NC_000086.7 X 140983373 + Atg4a NNNCCATCCTAAATGAAGCA NGG 2 0.5042 Tier II
7 NC_000084.6 18 16802398 + Cdh2 NNNCAATTAAAAATGAAGCA NGG 2 0.5 Tier II
8 NC_000081.6 15 48290938 + Csmd3 NNNCCATAAAAAATGAAACA NGG 2 0.45 Tier II
9 NC_000072.6 6 88091044 - Rpn1 NNNTCATCAAAAATGAAGAA NGG 2 0.3429 Tier II
10 NC_000086.7 X 57098825 + Vgll1 NNNCCATTAAATATGAAGCA NGG 2 0.2917 Tier II
11 NC_000069.6 3 5223068 - Zfhx4 NNNCCATAAAAAATGAAGAA NGG 2 0.2786 Tier II
12 NC_000075.6 9 69218137 + Rora NNNCCATCCAAAATGAACCA NGG 2 0.2721 Tier II
13 NC_000083.6 17 28360380 + Tulp1 NNNCCCTCAGAAATGAAGCA NGG 2 0.2525 Tier II
14 NC_000074.6 8 115243234 - Wwox NNNCCATCAAATATGAATCA NGG 2 0.2222 Tier II
15 NC_000069.6 3 31297485 - Slc7a14 NNNCCATCAAAATAGAAGCA NGG 2 0.1857 Tier II
16 NC_000086.7 X 87322877 + Il1rapl1 NNNCCATCAGAAATGAAGCA NAG 2 0.144 Tier II
17 NC_000077.6 11 108948971 + Axin2 NNNCCATCAGAAATGAAGCC NGG 2 0.1263 Tier II
18 NC_000073.6 7 87864963 + Grm5 NNNCCATCAAAAATTAAGTA NGG 2 0.0659 Tier II
19 NC_000077.6 11 121542967 - Tbcd NNNCCAACAAAAATGAAGCA NGA 2 0.0608 Tier II
20 NC_000086.7 X 147056402 - Htr2c NNNCCATCAAAATTGGAGCA NGG 2 0.0577 Tier II
21 NC_000078.6 12 57554755 + Ttc6 NNNCCATCAGAAATGAAGCA NGA 2 0.0386 Tier II
22 NC_000085.6 19 55229923 + Gucy2g NNNCCATCAAACATGATGCA NGG 2 0.0351 Tier II
23 NC_000068.7 2 125962584 + Galk2 NNNCCATCAAAAATGATGCA NGC 2 0.003 Tier II
24 NC_000085.6 19 58528837 - Ccdc172 NNNCCATCAAAAATGATGCA NGC 2 0.003 Tier II
25 NC_000079.6 13 24328670 - Cmah NNNCCATCAAAAAGGAACCA NGG 2 0.0 Tier II
26 NC_000071.6 5 129592950 + Mmp17 NNNCCATCAAAAAGGAAGCA NGT 2 0.0 Tier II
27 NC_000078.6 12 51010042 + 1700008C04Rik NNNCCAGCAAAAATAAAGCA NGG 2 0.5536 Tier III
28 NC_000086.7 X 87322877 + Gm18920 NNNCCATCAGAAATGAAGCA NAG 2 0.144 Tier III
29 NC_000070.6 4 106048317 + Gm30266 NNNCCAGCAAAAATGAGGCA NGG 2 0.1038 Tier III
30 NC_000084.6 18 21320432 - Gm41675 NNNCCATCAAATATGAAGCA NAG 2 0.0864 Tier III
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Other clones with same target sequence:

(none)