Construct: sgRNA BRDN0001145693
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTCCATCAAAAATGAAGCA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- PRKCQ (5588)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000010.11 | 10 | 6485189 | - | PRKCQ | NNNCCATCAAAAATGAAGCA | NGG | 0 | 1.0 | Tier I |
2 | NC_000003.12 | 3 | 47909801 | - | MAP4 | NNNCCATGAAAAATGAAGCA | NGG | 1 | 0.6429 | Tier I |
3 | NC_000012.12 | 12 | 116974638 | + | FBXW8 | NNNCCAATAAAAATGAAGCA | NGG | 2 | 0.7656 | Tier II |
4 | NC_000012.12 | 12 | 125379831 | + | TMEM132B | NNNCCATCATGAATGAAGCA | NGG | 2 | 0.5735 | Tier II |
5 | NC_000017.11 | 17 | 80863962 | + | RPTOR | NNNCTAACAAAAATGAAGCA | NGG | 2 | 0.5568 | Tier II |
6 | NC_000011.10 | 11 | 125405424 | + | PKNOX2 | NNNCTATTAAAAATGAAGCA | NGG | 2 | 0.5568 | Tier II |
7 | NC_000001.11 | 1 | 59337929 | - | FGGY | NNNCCATCAGAAATGAAGCA | NGG | 1 | 0.5556 | Tier II |
8 | NC_000020.11 | 20 | 46724168 | - | SLC2A10 | NNNTCATAAAAAATGAAGCA | NGG | 2 | 0.52 | Tier II |
9 | NC_000003.12 | 3 | 68978304 | + | EOGT | NNNCAATTAAAAATGAAGCA | NGG | 2 | 0.5 | Tier II |
10 | NC_000002.12 | 2 | 20672029 | - | GDF7 | NNNCTTTCAAAAATGAAGCA | NGG | 2 | 0.4545 | Tier II |
11 | NC_000001.11 | 1 | 71955492 | + | NEGR1 | NNNCCACCAAAAGTGAAGCA | NGG | 2 | 0.4484 | Tier II |
12 | NC_000004.12 | 4 | 81141890 | + | PRKG2 | NNNCCATCAGAGATGAAGCA | NGG | 2 | 0.4012 | Tier II |
13 | NC_000006.12 | 6 | 45006378 | - | SUPT3H | NNNCCATTAAAAATGAAGAA | NGG | 2 | 0.375 | Tier II |
14 | NC_000019.10 | 19 | 41399525 | - | BCKDHA | NNNCCACCAAAAATGAAGTA | NGG | 2 | 0.3173 | Tier II |
15 | NC_000005.10 | 5 | 10721670 | + | DAP | NNNCCCTCAAGAATGAAGCA | NGG | 2 | 0.2955 | Tier II |
16 | NC_000012.12 | 12 | 108246713 | + | WSCD2 | NNNCCATGAAAAATGAAGAA | NGG | 2 | 0.2755 | Tier II |
17 | NC_000023.11 | X | 33309904 | + | DMD | NNNCCATCAAAAATGAAGCA | NAG | 1 | 0.2593 | Tier II |
18 | NC_000013.11 | 13 | 33382101 | + | STARD13 | NNNCCATCATAAACGAAGCA | NGG | 2 | 0.2521 | Tier II |
19 | NC_000003.12 | 3 | 111751345 | + | PHLDB2 | NNNCCATTAAAAATGAAGCA | NAG | 2 | 0.2269 | Tier II |
20 | NC_000003.12 | 3 | 49533457 | - | DAG1 | NNNCCATCAAAAATGAAGCG | NAG | 2 | 0.1983 | Tier II |
21 | NC_000023.11 | X | 108123772 | + | ATG4A | NNNCCATCCCAAATGAAGCA | NGG | 2 | 0.1905 | Tier II |
22 | NC_000003.12 | 3 | 10853798 | - | SLC6A11 | NNNCCATCAAACATGAATCA | NGG | 2 | 0.1754 | Tier II |
23 | NC_000008.11 | 8 | 27830436 | + | PBK | NNNCTATCAAAAATGAAGCA | NAG | 2 | 0.165 | Tier II |
24 | NC_000016.10 | 16 | 71032772 | - | HYDIN | NNNGCATCAAAAATGAAGCA | NAG | 2 | 0.1296 | Tier II |
25 | NC_000020.11 | 20 | 42672545 | + | PTPRT | NNNCCATCTAAACTGAAGCA | NGG | 2 | 0.1263 | Tier II |
26 | NC_000006.12 | 6 | 131690386 | + | ENPP3 | NNNCCATCAACATTGAAGCA | NGG | 2 | 0.12 | Tier II |
27 | NC_000003.12 | 3 | 114912195 | + | ZBTB20 | NNNCCTTCAAAAATGAAGGA | NGG | 2 | 0.0893 | Tier II |
28 | NC_000008.11 | 8 | 1481212 | + | DLGAP2 | NNNCCATCAAAAATGAAAGA | NGG | 2 | 0.0865 | Tier II |
29 | NC_000008.11 | 8 | 76685312 | - | ZFHX4 | NNNCCATAAAAAATGAAGGA | NGG | 2 | 0.0813 | Tier II |
30 | NC_000005.10 | 5 | 93992189 | + | ARB2A | NNNCCTTCAAAAATGAAGCA | NGA | 2 | 0.0496 | Tier II |
31 | NC_000017.11 | 17 | 9163715 | + | NTN1 | NNNCCAACAAAAATGAAGCA | NTG | 2 | 0.0341 | Tier II |
32 | NC_000002.12 | 2 | 50258075 | - | NRXN1 | NNNCCATCAACAATGAAGCA | NGA | 2 | 0.0278 | Tier II |
33 | NC_000013.11 | 13 | 25733449 | - | ATP8A2 | NNNCCATGAAAAATGAAGCA | NGC | 2 | 0.0143 | Tier II |
34 | NC_000008.11 | 8 | 10195810 | - | MSRA | NNNCCATCAAAAATGGAGCA | NGA | 2 | 0.0134 | Tier II |
35 | NC_000022.11 | 22 | 32061810 | - | SLC5A1 | NNNCCATCCAAAATGAAGCA | NGC | 2 | 0.0127 | Tier II |
36 | NC_000008.11 | 8 | 96812378 | + | CPQ | NNNCCTTCAAAAATGAAGCA | NGT | 2 | 0.0115 | Tier II |
37 | NC_000008.11 | 8 | 80044581 | + | TPD52 | NNNCCCTCAAAAATGAAGCA | NGC | 2 | 0.0101 | Tier II |
38 | NC_000008.11 | 8 | 80044581 | + | TPD52-MRPS28 | NNNCCCTCAAAAATGAAGCA | NGC | 2 | 0.0101 | Tier II |
39 | NC_000007.14 | 7 | 39182921 | + | POU6F2 | NNNCCAGCAAAAATGCAGCA | NGG | 2 | 0.0 | Tier II |
40 | NC_000012.12 | 12 | 130606376 | + | RIMBP2 | NNNCCATAAAAAAGGAAGCA | NGG | 2 | 0.0 | Tier II |
41 | NC_000002.12 | 2 | 205291734 | + | PARD3B | NNNCCATCAAAAAGGAAGCA | NAG | 2 | 0.0 | Tier II |
42 | NC_000018.10 | 18 | 76886082 | - | ZNF236 | NNNCCATCAAAAAGGAAGCA | NGC | 2 | 0.0 | Tier II |
43 | NC_000002.12 | 2 | 224712880 | - | CCDC195 | NNNCCATCAAAAAGGAAGGA | NGG | 2 | 0.0 | Tier II |
44 | NC_000007.14 | 7 | 36433734 | - | ANLN | NNNCCATCAAAAATGAAGCA | NAA | 2 | 0.0 | Tier II |
45 | NC_000003.12 | 3 | 67562045 | - | SUCLG2 | NNNCCATCAAAAATGCAGAA | NGG | 2 | 0.0 | Tier II |
46 | NC_000011.10 | 11 | 19511548 | + | NAV2 | NNNCCATCAAAATTGTAGCA | NGG | 2 | 0.0 | Tier II |
47 | NC_000007.14 | 7 | 86854837 | + | GRM3 | NNNCCATCAAAGAGGAAGCA | NGG | 2 | 0.0 | Tier II |
48 | NC_000010.11 | 10 | 122217736 | - | TACC2 | NNNCTATCAAAAAGGAAGCA | NGG | 2 | 0.0 | Tier II |
49 | NC_000010.11 | 10 | 37409986 | + | LOC107984223 | NNNCTAACAAAAATGAAGCA | NGG | 2 | 0.5568 | Tier III |
50 | NC_000012.12 | 12 | 10727913 | + | LOC112268104 | NNNCCCTCAAAAATAAAGCA | NGG | 2 | 0.4278 | Tier III |
51 | NC_000008.11 | 8 | 80225460 | - | LOC105375920 | NNNCCATCCAAAATGAAACA | NGG | 2 | 0.3956 | Tier III |
52 | NC_000006.12 | 6 | 93740861 | + | TSG1 | NNNCAATCAAAAATGAACCA | NGG | 2 | 0.2721 | Tier III |
53 | NC_000002.12 | 2 | 21073048 | + | LOC124905593 | NNNCCATCAGCAATGAAGCA | NGG | 2 | 0.2222 | Tier III |
54 | NC_000011.10 | 11 | 105466203 | + | LOC105369468 | NNNCCATAAAAAATGAAGCA | NAG | 2 | 0.1685 | Tier III |
55 | NC_000014.9 | 14 | 46600934 | - | LOC105370481 | NNNCCCTCAAATATGAAGCA | NGG | 2 | 0.1515 | Tier III |
56 | NC_000006.12 | 6 | 127610619 | + | LOC105377996 | NNNCCATGAAAACTGAAGCA | NGG | 2 | 0.1353 | Tier III |
57 | NC_000001.11 | 1 | 146678154 | + | HYDIN2 | NNNGCATCAAAAATGAAGCA | NAG | 2 | 0.1296 | Tier III |
58 | NC_000005.10 | 5 | 124331013 | - | LINC01170 | NNNCCATCAACAATGAGGCA | NGG | 2 | 0.0706 | Tier III |
59 | NC_000013.11 | 13 | 62888621 | + | LOC105370234 | NNNCTATCAAAAATGAAGCA | NGA | 2 | 0.0442 | Tier III |
60 | NC_000007.14 | 7 | 94323465 | + | LOC107986821 | NNNCCATCAAAAATTAAGCA | NGA | 2 | 0.0099 | Tier III |
61 | NC_000007.14 | 7 | 94323465 | + | LOC112267858 | NNNCCATCAAAAATTAAGCA | NGA | 2 | 0.0099 | Tier III |
62 | NC_000018.10 | 18 | 76886082 | - | LOC100421894 | NNNCCATCAAAAAGGAAGCA | NGC | 2 | 0.0 | Tier III |
63 | NC_000002.12 | 2 | 224712880 | - | LOC105373910 | NNNCCATCAAAAAGGAAGGA | NGG | 2 | 0.0 | Tier III |
64 | NC_000011.10 | 11 | 19511548 | + | NAV2-AS4 | NNNCCATCAAAATTGTAGCA | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000068.7 | 2 | 21827195 | - | Gpr158 | NNNCCTTCAAAAACGAAGCA | NGG | 2 | 0.2041 | Tier I |
2 | NC_000071.6 | 5 | 73023894 | - | Fryl | NNNCCATCAAGAATGAAGCA | NAG | 2 | 0.1685 | Tier I |
3 | NC_000072.6 | 6 | 63507062 | - | Grid2 | NNNCCACCAAAAATAAAGCA | NGG | 2 | 0.6471 | Tier II |
4 | NC_000068.7 | 2 | 26277555 | + | Ccdc187 | NNNCCAACAAAAATGAAGCT | NGG | 2 | 0.525 | Tier II |
5 | NC_000084.6 | 18 | 82668006 | + | Zfp236 | NNNACATCTAAAATGAAGCA | NGG | 2 | 0.5053 | Tier II |
6 | NC_000086.7 | X | 140983373 | + | Atg4a | NNNCCATCCTAAATGAAGCA | NGG | 2 | 0.5042 | Tier II |
7 | NC_000084.6 | 18 | 16802398 | + | Cdh2 | NNNCAATTAAAAATGAAGCA | NGG | 2 | 0.5 | Tier II |
8 | NC_000081.6 | 15 | 48290938 | + | Csmd3 | NNNCCATAAAAAATGAAACA | NGG | 2 | 0.45 | Tier II |
9 | NC_000072.6 | 6 | 88091044 | - | Rpn1 | NNNTCATCAAAAATGAAGAA | NGG | 2 | 0.3429 | Tier II |
10 | NC_000086.7 | X | 57098825 | + | Vgll1 | NNNCCATTAAATATGAAGCA | NGG | 2 | 0.2917 | Tier II |
11 | NC_000069.6 | 3 | 5223068 | - | Zfhx4 | NNNCCATAAAAAATGAAGAA | NGG | 2 | 0.2786 | Tier II |
12 | NC_000075.6 | 9 | 69218137 | + | Rora | NNNCCATCCAAAATGAACCA | NGG | 2 | 0.2721 | Tier II |
13 | NC_000083.6 | 17 | 28360380 | + | Tulp1 | NNNCCCTCAGAAATGAAGCA | NGG | 2 | 0.2525 | Tier II |
14 | NC_000074.6 | 8 | 115243234 | - | Wwox | NNNCCATCAAATATGAATCA | NGG | 2 | 0.2222 | Tier II |
15 | NC_000069.6 | 3 | 31297485 | - | Slc7a14 | NNNCCATCAAAATAGAAGCA | NGG | 2 | 0.1857 | Tier II |
16 | NC_000086.7 | X | 87322877 | + | Il1rapl1 | NNNCCATCAGAAATGAAGCA | NAG | 2 | 0.144 | Tier II |
17 | NC_000077.6 | 11 | 108948971 | + | Axin2 | NNNCCATCAGAAATGAAGCC | NGG | 2 | 0.1263 | Tier II |
18 | NC_000073.6 | 7 | 87864963 | + | Grm5 | NNNCCATCAAAAATTAAGTA | NGG | 2 | 0.0659 | Tier II |
19 | NC_000077.6 | 11 | 121542967 | - | Tbcd | NNNCCAACAAAAATGAAGCA | NGA | 2 | 0.0608 | Tier II |
20 | NC_000086.7 | X | 147056402 | - | Htr2c | NNNCCATCAAAATTGGAGCA | NGG | 2 | 0.0577 | Tier II |
21 | NC_000078.6 | 12 | 57554755 | + | Ttc6 | NNNCCATCAGAAATGAAGCA | NGA | 2 | 0.0386 | Tier II |
22 | NC_000085.6 | 19 | 55229923 | + | Gucy2g | NNNCCATCAAACATGATGCA | NGG | 2 | 0.0351 | Tier II |
23 | NC_000068.7 | 2 | 125962584 | + | Galk2 | NNNCCATCAAAAATGATGCA | NGC | 2 | 0.003 | Tier II |
24 | NC_000085.6 | 19 | 58528837 | - | Ccdc172 | NNNCCATCAAAAATGATGCA | NGC | 2 | 0.003 | Tier II |
25 | NC_000079.6 | 13 | 24328670 | - | Cmah | NNNCCATCAAAAAGGAACCA | NGG | 2 | 0.0 | Tier II |
26 | NC_000071.6 | 5 | 129592950 | + | Mmp17 | NNNCCATCAAAAAGGAAGCA | NGT | 2 | 0.0 | Tier II |
27 | NC_000078.6 | 12 | 51010042 | + | 1700008C04Rik | NNNCCAGCAAAAATAAAGCA | NGG | 2 | 0.5536 | Tier III |
28 | NC_000086.7 | X | 87322877 | + | Gm18920 | NNNCCATCAGAAATGAAGCA | NAG | 2 | 0.144 | Tier III |
29 | NC_000070.6 | 4 | 106048317 | + | Gm30266 | NNNCCAGCAAAAATGAGGCA | NGG | 2 | 0.1038 | Tier III |
30 | NC_000084.6 | 18 | 21320432 | - | Gm41675 | NNNCCATCAAATATGAAGCA | NAG | 2 | 0.0864 | Tier III |