Construct: sgRNA BRDN0001145728

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTCATATGAGCCAGTGACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DLG3 (1741)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76157

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 70498558 + DLG3 NNNCATATGAGCCAGTGACA NGG 0 1.0 Tier I
2 NC_000001.11 1 23079522 - KDM1A NNNCACATGAGCCAGTGACA NGG 1 0.9091 Tier II
3 NC_000002.12 2 25337586 - DNMT3A NNNCACATGAGCCAGTGTCA NGG 2 0.4545 Tier II
4 NC_000012.12 12 50743984 - DIP2B NNNCATTTGAACCAGTGACA NGG 2 0.4375 Tier II
5 NC_000001.11 1 17416299 + RCC2 NNNCATAGGAGGCAGTGACA NGG 2 0.3259 Tier II
6 NC_000001.11 1 41973770 + HIVEP3 NNNCATAGGAGCCAGTGGCA NGG 2 0.2933 Tier II
7 NC_000013.11 13 66903975 + PCDH9 NNNCATATTAGCCTGTGACA NGG 2 0.2844 Tier II
8 NC_000023.11 X 70028350 + EDA NNNCATATGAGCCAGAGACA NAG 2 0.2357 Tier II
9 NC_000008.11 8 133276426 + NDRG1 NNNCATATGATCCAGTGATA NGG 2 0.1775 Tier II
10 NC_000014.9 14 24904137 - STXBP6 NNNCATATGAGCGAGTGACA NGG 1 0.1364 Tier II
11 NC_000023.11 X 150888512 + CD99L2 NNNCAGATGAGCCAGTGCCA NGG 2 0.1088 Tier II
12 NC_000003.12 3 25363917 - RARB NNNCATTTGAGCCAGTGCCA NGG 2 0.0833 Tier II
13 NC_000023.11 X 32285047 + DMD NNNCATATGACCCAGTGAGA NGG 2 0.0536 Tier II
14 NC_000008.11 8 66127049 - TRIM55 NNNCATATAAGCCAGTGACA NGA 2 0.0446 Tier II
15 NC_000002.12 2 143616453 + ARHGAP15 NNNCATATGAGCCTGTGACA NGC 2 0.0119 Tier II
16 NC_000015.10 15 57030892 - TCF12 NNNCATATGAGCCAGTTACA NTG 2 0.0097 Tier II
17 NC_000012.12 12 50743984 - LOC124902932 NNNCATTTGAACCAGTGACA NGG 2 0.4375 Tier III
18 NC_000010.11 10 36063678 - LOC107984222 NNNCGTATGAGCCAGTGAAA NGG 2 0.3086 Tier III
19 NC_000013.11 13 66903975 + PCDH9-AS2 NNNCATATTAGCCTGTGACA NGG 2 0.2844 Tier III
20 NC_000008.11 8 103268396 - LOC105369147 NNNCATCTGAGCCAGTGACT NGG 2 0.2625 Tier III
21 NC_000006.12 6 102444422 - TARDBPP5 NNNCATATGAGCCAGTGTAA NGG 2 0.2143 Tier III
22 NC_000002.12 2 114008576 - LINC01191 NNNCATATGAGCCAGTGCCT NGG 2 0.1143 Tier III
23 NC_000001.11 1 72363613 + LOC105378797 NNNCTTATGAGCCAGTGACA NAG 2 0.0943 Tier III
24 NC_000023.11 X 22604607 + PTCHD1-AS NNNCATATAAGCCAGTGACA NTG 2 0.025 Tier III
25 NC_000011.10 11 24140133 - LOC107984378 NNNCAGATGAGCCAGTGACA NTG 2 0.0223 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 100812764 + Dlg3 NNNCATATGAGCCAGTGACA NGA 1 0.0694 Tier I
2 NC_000076.6 10 31927471 - Nkain2 NNNTACATGAGCCAGTGACA NGG 2 0.7273 Tier II
3 NC_000069.6 3 145813777 + Ddah1 NNNAATATGAGCCAGTGACG NGG 2 0.644 Tier II
4 NC_000074.6 8 41301029 - Pcm1 NNNCATACGAGCCAGTGACT NGG 2 0.6 Tier II
5 NC_000082.6 16 58730731 - Cldnd1 NNNGATATGAGCCAGTGACA NGG 1 0.5 Tier II
6 NC_000082.6 16 59595232 + Crybg3 NNNAATATGGGCCAGTGACA NGG 2 0.4678 Tier II
7 NC_000073.6 7 81253454 + Pde8a NNNCATGTGAGCCAGTGACT NGG 2 0.4235 Tier II
8 NC_000078.6 12 16788629 - Greb1 NNNCATCTGAGCCAGAGACA NGG 2 0.3977 Tier II
9 NC_000086.7 X 85583366 - Tab3 NNNCATATGAGCAAGTGACA NGG 1 0.3846 Tier II
10 NC_000073.6 7 24978640 + Atp1a3 NNNCATATGAGCAAGAGACA NGG 2 0.3497 Tier II
11 NC_000075.6 9 49760183 + Ncam1 NNNAATATGAGCAAGTGACA NGG 2 0.3239 Tier II
12 NC_000073.6 7 74392412 + Slco3a1 NNNTATATGATCCAGTGACA NGG 2 0.3077 Tier II
13 NC_000084.6 18 74519193 + Myo5b NNNCATCTGAGCCTGTGACA NGG 2 0.2333 Tier II
14 NC_000070.6 4 8564916 - Rab2a NNNCATATGAGTCAGTGAAA NGG 2 0.2308 Tier II
15 NC_000073.6 7 89141273 - Tmem135 NNNCATTTGAGCCAGTGTCA NGG 2 0.2188 Tier II
16 NC_000068.7 2 152320872 + Rbck1 NNNCCTCTGAGCCAGTGACA NGG 2 0.2188 Tier II
17 NC_000068.7 2 28007599 - Col5a1 NNNCATATCAGCCAGTGGCA NGG 2 0.2154 Tier II
18 NC_000083.6 17 29605564 + Tbc1d22b NNNCAGATGAGCCAGTGACA NAG 2 0.1481 Tier II
19 NC_000083.6 17 46442849 + Ttbk1 NNNCATCTGAGCCAGTGACA NAG 2 0.1134 Tier II
20 NC_000071.6 5 81551663 - Adgrl3 NNNCTTATGAGCCAGTTACA NGG 2 0.0909 Tier II
21 NC_000074.6 8 61397120 - Sh3rf1 NNNCATATGAGCCAGCGAGA NGG 2 0.0833 Tier II
22 NC_000072.6 6 120240748 + B4galnt3 NNNCATCTGAGCCAGTGCCA NGG 2 0.0833 Tier II
23 NC_000070.6 4 46795333 - Gabbr2 NNNCATATGAGTGAGTGACA NGG 2 0.0734 Tier II
24 NC_000070.6 4 14781371 - Lrrc69 NNNCAGATGAGCCAGTGAGA NGG 2 0.0714 Tier II
25 NC_000069.6 3 83335268 - Dchs2 NNNCATATGAGCCAGTTACA NAG 2 0.0648 Tier II
26 NC_000082.6 16 24400726 - Lpp NNNCCTATGAGCCAGTGAGA NGG 2 0.0625 Tier II
27 NC_000071.6 5 117573230 - Ksr2 NNNGATATGAGCCAGTGAGA NGG 2 0.0625 Tier II
28 NC_000074.6 8 92888974 + Lpcat2 NNNCATATGAGGCAGTGACA NTG 2 0.0173 Tier II
29 NC_000071.6 5 38859422 + Clnk NNNCATATGAGCCAGTGGCA NTG 2 0.0156 Tier II
30 NC_000069.6 3 19644381 - Trim55 NNNCATATAAGCCAGTGACA NGC 2 0.0143 Tier II
31 NC_000076.6 10 96332423 - Gm33782 NNNCAGATAAGCCAGTGACA NGG 2 0.3673 Tier III
32 NC_000075.6 9 100275329 + Gm28167 NNNCATCTGAGCCAGTGACT NGG 2 0.2625 Tier III
33 NC_000084.6 18 74519193 + 1700120E14Rik NNNCATCTGAGCCTGTGACA NGG 2 0.2333 Tier III
34 NC_000068.7 2 166502723 + Gm14267 NNNCATATGAGCCAGTAACC NGG 2 0.2121 Tier III
35 NC_000073.6 7 83368247 + Gm29976 NNNCATATGGGCCAGTGAGA NGG 2 0.0694 Tier III
36 NC_000073.6 7 15307975 + Obox4-ps24 NNNCATCTGAGCCAGTGACA NTG 2 0.017 Tier III
37 NC_000073.6 7 15643131 + Obox4-ps32 NNNCATCTGAGCCAGTGACA NTG 2 0.017 Tier III
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Other clones with same target sequence:

(none)