Construct: sgRNA BRDN0001145728
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTCATATGAGCCAGTGACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DLG3 (1741)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76157
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000023.11 |
X |
70498558 |
+ |
DLG3 |
NNNCATATGAGCCAGTGACA |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000001.11 |
1 |
23079522 |
- |
KDM1A |
NNNCACATGAGCCAGTGACA |
NGG |
1 |
0.9091 |
Tier II |
3 |
NC_000002.12 |
2 |
25337586 |
- |
DNMT3A |
NNNCACATGAGCCAGTGTCA |
NGG |
2 |
0.4545 |
Tier II |
4 |
NC_000012.12 |
12 |
50743984 |
- |
DIP2B |
NNNCATTTGAACCAGTGACA |
NGG |
2 |
0.4375 |
Tier II |
5 |
NC_000001.11 |
1 |
17416299 |
+ |
RCC2 |
NNNCATAGGAGGCAGTGACA |
NGG |
2 |
0.3259 |
Tier II |
6 |
NC_000001.11 |
1 |
41973770 |
+ |
HIVEP3 |
NNNCATAGGAGCCAGTGGCA |
NGG |
2 |
0.2933 |
Tier II |
7 |
NC_000013.11 |
13 |
66903975 |
+ |
PCDH9 |
NNNCATATTAGCCTGTGACA |
NGG |
2 |
0.2844 |
Tier II |
8 |
NC_000023.11 |
X |
70028350 |
+ |
EDA |
NNNCATATGAGCCAGAGACA |
NAG |
2 |
0.2357 |
Tier II |
9 |
NC_000008.11 |
8 |
133276426 |
+ |
NDRG1 |
NNNCATATGATCCAGTGATA |
NGG |
2 |
0.1775 |
Tier II |
10 |
NC_000014.9 |
14 |
24904137 |
- |
STXBP6 |
NNNCATATGAGCGAGTGACA |
NGG |
1 |
0.1364 |
Tier II |
11 |
NC_000023.11 |
X |
150888512 |
+ |
CD99L2 |
NNNCAGATGAGCCAGTGCCA |
NGG |
2 |
0.1088 |
Tier II |
12 |
NC_000003.12 |
3 |
25363917 |
- |
RARB |
NNNCATTTGAGCCAGTGCCA |
NGG |
2 |
0.0833 |
Tier II |
13 |
NC_000023.11 |
X |
32285047 |
+ |
DMD |
NNNCATATGACCCAGTGAGA |
NGG |
2 |
0.0536 |
Tier II |
14 |
NC_000008.11 |
8 |
66127049 |
- |
TRIM55 |
NNNCATATAAGCCAGTGACA |
NGA |
2 |
0.0446 |
Tier II |
15 |
NC_000002.12 |
2 |
143616453 |
+ |
ARHGAP15 |
NNNCATATGAGCCTGTGACA |
NGC |
2 |
0.0119 |
Tier II |
16 |
NC_000015.10 |
15 |
57030892 |
- |
TCF12 |
NNNCATATGAGCCAGTTACA |
NTG |
2 |
0.0097 |
Tier II |
17 |
NC_000012.12 |
12 |
50743984 |
- |
LOC124902932 |
NNNCATTTGAACCAGTGACA |
NGG |
2 |
0.4375 |
Tier III |
18 |
NC_000010.11 |
10 |
36063678 |
- |
LOC107984222 |
NNNCGTATGAGCCAGTGAAA |
NGG |
2 |
0.3086 |
Tier III |
19 |
NC_000013.11 |
13 |
66903975 |
+ |
PCDH9-AS2 |
NNNCATATTAGCCTGTGACA |
NGG |
2 |
0.2844 |
Tier III |
20 |
NC_000008.11 |
8 |
103268396 |
- |
LOC105369147 |
NNNCATCTGAGCCAGTGACT |
NGG |
2 |
0.2625 |
Tier III |
21 |
NC_000006.12 |
6 |
102444422 |
- |
TARDBPP5 |
NNNCATATGAGCCAGTGTAA |
NGG |
2 |
0.2143 |
Tier III |
22 |
NC_000002.12 |
2 |
114008576 |
- |
LINC01191 |
NNNCATATGAGCCAGTGCCT |
NGG |
2 |
0.1143 |
Tier III |
23 |
NC_000001.11 |
1 |
72363613 |
+ |
LOC105378797 |
NNNCTTATGAGCCAGTGACA |
NAG |
2 |
0.0943 |
Tier III |
24 |
NC_000023.11 |
X |
22604607 |
+ |
PTCHD1-AS |
NNNCATATAAGCCAGTGACA |
NTG |
2 |
0.025 |
Tier III |
25 |
NC_000011.10 |
11 |
24140133 |
- |
LOC107984378 |
NNNCAGATGAGCCAGTGACA |
NTG |
2 |
0.0223 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000086.7 |
X |
100812764 |
+ |
Dlg3 |
NNNCATATGAGCCAGTGACA |
NGA |
1 |
0.0694 |
Tier I |
2 |
NC_000076.6 |
10 |
31927471 |
- |
Nkain2 |
NNNTACATGAGCCAGTGACA |
NGG |
2 |
0.7273 |
Tier II |
3 |
NC_000069.6 |
3 |
145813777 |
+ |
Ddah1 |
NNNAATATGAGCCAGTGACG |
NGG |
2 |
0.644 |
Tier II |
4 |
NC_000074.6 |
8 |
41301029 |
- |
Pcm1 |
NNNCATACGAGCCAGTGACT |
NGG |
2 |
0.6 |
Tier II |
5 |
NC_000082.6 |
16 |
58730731 |
- |
Cldnd1 |
NNNGATATGAGCCAGTGACA |
NGG |
1 |
0.5 |
Tier II |
6 |
NC_000082.6 |
16 |
59595232 |
+ |
Crybg3 |
NNNAATATGGGCCAGTGACA |
NGG |
2 |
0.4678 |
Tier II |
7 |
NC_000073.6 |
7 |
81253454 |
+ |
Pde8a |
NNNCATGTGAGCCAGTGACT |
NGG |
2 |
0.4235 |
Tier II |
8 |
NC_000078.6 |
12 |
16788629 |
- |
Greb1 |
NNNCATCTGAGCCAGAGACA |
NGG |
2 |
0.3977 |
Tier II |
9 |
NC_000086.7 |
X |
85583366 |
- |
Tab3 |
NNNCATATGAGCAAGTGACA |
NGG |
1 |
0.3846 |
Tier II |
10 |
NC_000073.6 |
7 |
24978640 |
+ |
Atp1a3 |
NNNCATATGAGCAAGAGACA |
NGG |
2 |
0.3497 |
Tier II |
11 |
NC_000075.6 |
9 |
49760183 |
+ |
Ncam1 |
NNNAATATGAGCAAGTGACA |
NGG |
2 |
0.3239 |
Tier II |
12 |
NC_000073.6 |
7 |
74392412 |
+ |
Slco3a1 |
NNNTATATGATCCAGTGACA |
NGG |
2 |
0.3077 |
Tier II |
13 |
NC_000084.6 |
18 |
74519193 |
+ |
Myo5b |
NNNCATCTGAGCCTGTGACA |
NGG |
2 |
0.2333 |
Tier II |
14 |
NC_000070.6 |
4 |
8564916 |
- |
Rab2a |
NNNCATATGAGTCAGTGAAA |
NGG |
2 |
0.2308 |
Tier II |
15 |
NC_000073.6 |
7 |
89141273 |
- |
Tmem135 |
NNNCATTTGAGCCAGTGTCA |
NGG |
2 |
0.2188 |
Tier II |
16 |
NC_000068.7 |
2 |
152320872 |
+ |
Rbck1 |
NNNCCTCTGAGCCAGTGACA |
NGG |
2 |
0.2188 |
Tier II |
17 |
NC_000068.7 |
2 |
28007599 |
- |
Col5a1 |
NNNCATATCAGCCAGTGGCA |
NGG |
2 |
0.2154 |
Tier II |
18 |
NC_000083.6 |
17 |
29605564 |
+ |
Tbc1d22b |
NNNCAGATGAGCCAGTGACA |
NAG |
2 |
0.1481 |
Tier II |
19 |
NC_000083.6 |
17 |
46442849 |
+ |
Ttbk1 |
NNNCATCTGAGCCAGTGACA |
NAG |
2 |
0.1134 |
Tier II |
20 |
NC_000071.6 |
5 |
81551663 |
- |
Adgrl3 |
NNNCTTATGAGCCAGTTACA |
NGG |
2 |
0.0909 |
Tier II |
21 |
NC_000074.6 |
8 |
61397120 |
- |
Sh3rf1 |
NNNCATATGAGCCAGCGAGA |
NGG |
2 |
0.0833 |
Tier II |
22 |
NC_000072.6 |
6 |
120240748 |
+ |
B4galnt3 |
NNNCATCTGAGCCAGTGCCA |
NGG |
2 |
0.0833 |
Tier II |
23 |
NC_000070.6 |
4 |
46795333 |
- |
Gabbr2 |
NNNCATATGAGTGAGTGACA |
NGG |
2 |
0.0734 |
Tier II |
24 |
NC_000070.6 |
4 |
14781371 |
- |
Lrrc69 |
NNNCAGATGAGCCAGTGAGA |
NGG |
2 |
0.0714 |
Tier II |
25 |
NC_000069.6 |
3 |
83335268 |
- |
Dchs2 |
NNNCATATGAGCCAGTTACA |
NAG |
2 |
0.0648 |
Tier II |
26 |
NC_000082.6 |
16 |
24400726 |
- |
Lpp |
NNNCCTATGAGCCAGTGAGA |
NGG |
2 |
0.0625 |
Tier II |
27 |
NC_000071.6 |
5 |
117573230 |
- |
Ksr2 |
NNNGATATGAGCCAGTGAGA |
NGG |
2 |
0.0625 |
Tier II |
28 |
NC_000074.6 |
8 |
92888974 |
+ |
Lpcat2 |
NNNCATATGAGGCAGTGACA |
NTG |
2 |
0.0173 |
Tier II |
29 |
NC_000071.6 |
5 |
38859422 |
+ |
Clnk |
NNNCATATGAGCCAGTGGCA |
NTG |
2 |
0.0156 |
Tier II |
30 |
NC_000069.6 |
3 |
19644381 |
- |
Trim55 |
NNNCATATAAGCCAGTGACA |
NGC |
2 |
0.0143 |
Tier II |
31 |
NC_000076.6 |
10 |
96332423 |
- |
Gm33782 |
NNNCAGATAAGCCAGTGACA |
NGG |
2 |
0.3673 |
Tier III |
32 |
NC_000075.6 |
9 |
100275329 |
+ |
Gm28167 |
NNNCATCTGAGCCAGTGACT |
NGG |
2 |
0.2625 |
Tier III |
33 |
NC_000084.6 |
18 |
74519193 |
+ |
1700120E14Rik |
NNNCATCTGAGCCTGTGACA |
NGG |
2 |
0.2333 |
Tier III |
34 |
NC_000068.7 |
2 |
166502723 |
+ |
Gm14267 |
NNNCATATGAGCCAGTAACC |
NGG |
2 |
0.2121 |
Tier III |
35 |
NC_000073.6 |
7 |
83368247 |
+ |
Gm29976 |
NNNCATATGGGCCAGTGAGA |
NGG |
2 |
0.0694 |
Tier III |
36 |
NC_000073.6 |
7 |
15307975 |
+ |
Obox4-ps24 |
NNNCATCTGAGCCAGTGACA |
NTG |
2 |
0.017 |
Tier III |
37 |
NC_000073.6 |
7 |
15643131 |
+ |
Obox4-ps32 |
NNNCATCTGAGCCAGTGACA |
NTG |
2 |
0.017 |
Tier III |
Other clones with same target sequence:
(none)