Construct: sgRNA BRDN0001145765
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGGTGTCCCAATGAAAGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP4K1 (11184)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77609
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
38613901 |
+ |
MAP4K1 |
NNNGTGTCCCAATGAAAGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
179526930 |
- |
ZNF385B |
NNNGTATCCCAGTGAAAGAG |
NGG |
2 |
0.7222 |
Tier II |
| 3 |
NC_000007.14 |
7 |
121183438 |
+ |
CPED1 |
NNNGTATCCCAAAGAAAGAG |
NGG |
2 |
0.6923 |
Tier II |
| 4 |
NC_000013.11 |
13 |
110255505 |
+ |
COL4A1 |
NNNGTTTCCCAATGAAAGAA |
NGG |
2 |
0.625 |
Tier II |
| 5 |
NC_000002.12 |
2 |
140737738 |
+ |
LRP1B |
NNNGTGTCACAATGAAAGAC |
NGG |
2 |
0.3673 |
Tier II |
| 6 |
NC_000007.14 |
7 |
79178389 |
+ |
MAGI2 |
NNNATGTCCCTATGAAAGAG |
NGG |
2 |
0.2769 |
Tier II |
| 7 |
NC_000003.12 |
3 |
50173014 |
- |
SEMA3F |
NNNGGGTCCCAATGAAAGGG |
NGG |
2 |
0.24 |
Tier II |
| 8 |
NC_000012.12 |
12 |
121522960 |
- |
KDM2B |
NNNGTGTCCCAGTGAATGAG |
NGG |
2 |
0.0963 |
Tier II |
| 9 |
NC_000017.11 |
17 |
19663265 |
+ |
ALDH3A2 |
NNNGTGACCCAATGAAAGAG |
NTG |
2 |
0.0341 |
Tier II |
| 10 |
NC_000007.14 |
7 |
121183438 |
+ |
LOC124901735 |
NNNGTATCCCAAAGAAAGAG |
NGG |
2 |
0.6923 |
Tier III |
| 11 |
NC_000013.11 |
13 |
110255505 |
+ |
LOC124903212 |
NNNGTTTCCCAATGAAAGAA |
NGG |
2 |
0.625 |
Tier III |
| 12 |
NC_000008.11 |
8 |
40112657 |
+ |
LOC105379385 |
NNNGAGTCCAAATGAAAGAG |
NGG |
2 |
0.4333 |
Tier III |
| 13 |
NC_000023.11 |
X |
153779915 |
- |
LOC124905227 |
NNNGAGTCCCAATGAAAGGG |
NGG |
2 |
0.1875 |
Tier III |
| 14 |
NC_000004.12 |
4 |
138036509 |
+ |
LINC00616 |
NNNGTGTCCCAGTGAAAGAG |
NGT |
2 |
0.0116 |
Tier III |
| 15 |
NC_000004.12 |
4 |
138036509 |
+ |
LOC105377447 |
NNNGTGTCCCAGTGAAAGAG |
NGT |
2 |
0.0116 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
28987167 |
- |
Map4k1 |
NNNGTGTCCCAATGAAAGAA |
NGG |
1 |
0.9375 |
Tier I |
| 2 |
NC_000084.6 |
18 |
88972524 |
+ |
Rttn |
NNNCTGTCCCAATGAAAGAG |
NAG |
2 |
0.1373 |
Tier I |
| 3 |
NC_000082.6 |
16 |
55318901 |
+ |
Zpld1 |
NNNGTGTTTCAATGAAAGAG |
NGG |
2 |
0.7656 |
Tier II |
| 4 |
NC_000068.7 |
2 |
153953220 |
- |
Bpifb4 |
NNNGTCTCCCAATGAAAGAG |
NGG |
1 |
0.6818 |
Tier II |
| 5 |
NC_000081.6 |
15 |
40705563 |
- |
Zfpm2 |
NNNATTTCCCAATGAAAGAG |
NGG |
2 |
0.6 |
Tier II |
| 6 |
NC_000077.6 |
11 |
107758356 |
- |
Cacng4 |
NNNCTGTCTCAATGAAAGAG |
NGG |
2 |
0.4632 |
Tier II |
| 7 |
NC_000076.6 |
10 |
93251160 |
- |
Elk3 |
NNNGTGACCCAATGAAACAG |
NGG |
2 |
0.4167 |
Tier II |
| 8 |
NC_000084.6 |
18 |
62925941 |
+ |
Apcdd1 |
NNNGTGGGCCAATGAAAGAG |
NGG |
2 |
0.3782 |
Tier II |
| 9 |
NC_000078.6 |
12 |
87040705 |
+ |
Tmem63c |
NNNGTGTCTCCATGAAAGAG |
NGG |
2 |
0.35 |
Tier II |
| 10 |
NC_000075.6 |
9 |
96707798 |
+ |
Zbtb38 |
NNNGTGTCACAATGCAAGAG |
NGG |
2 |
0.2338 |
Tier II |
| 11 |
NC_000073.6 |
7 |
117549665 |
+ |
Xylt1 |
NNNGTGTCCCAAGGAAAGAT |
NGG |
2 |
0.1826 |
Tier II |
| 12 |
NC_000083.6 |
17 |
19668895 |
+ |
Vmn2r102 |
NNNGTGTCCCTATGCAAGAG |
NGG |
2 |
0.0839 |
Tier II |
| 13 |
NC_000083.6 |
17 |
19779771 |
- |
Vmn2r103 |
NNNGTGTCCCTATGCAAGAG |
NGG |
2 |
0.0839 |
Tier II |
| 14 |
NC_000080.6 |
14 |
10738983 |
+ |
Fhit |
NNNGTGTCCCCATGAAGGAG |
NGG |
2 |
0.0706 |
Tier II |
| 15 |
NC_000082.6 |
16 |
12178928 |
+ |
Shisa9 |
NNNGTGTCCCAAGGTAAGAG |
NGG |
2 |
0.0522 |
Tier II |
| 16 |
NC_000075.6 |
9 |
116584846 |
+ |
Rbms3 |
NNNTTGTCCCAATGAAAGAG |
NGA |
2 |
0.0253 |
Tier II |
| 17 |
NC_000077.6 |
11 |
35230920 |
- |
Slit3 |
NNNGTGTCCCAAAGAAAGAG |
NGT |
2 |
0.0112 |
Tier II |
| 18 |
NC_000071.6 |
5 |
5160015 |
+ |
Cdk14 |
NNNGTGTCCCAAGGAAAGAG |
NTG |
2 |
0.0102 |
Tier II |
| 19 |
NC_000080.6 |
14 |
10738983 |
+ |
Fhitos |
NNNGTGTCCCCATGAAGGAG |
NGG |
2 |
0.0706 |
Tier III |
Other clones with same target sequence:
(none)
