Construct: sgRNA BRDN0001146004
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TACGTCATTAAGAGTTCAAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80174
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
54655086 |
- |
SPTBN1 |
NNNTTCATTAAGAGTTCAAC |
NGA |
2 |
0.0253 |
Tier I |
| 2 |
NC_000011.10 |
11 |
4125906 |
+ |
RRM1 |
NNNGGCATCAAGAGTTCAAC |
NGG |
2 |
0.5908 |
Tier II |
| 3 |
NC_000021.9 |
21 |
45145812 |
- |
ADARB1 |
NNNGTCATTAAAAGTTCATC |
NGG |
2 |
0.5026 |
Tier II |
| 4 |
NC_000003.12 |
3 |
139473239 |
- |
RBP2 |
NNNATCATTAAGAGTTCAAA |
NGG |
2 |
0.45 |
Tier II |
| 5 |
NC_000007.14 |
7 |
114950186 |
+ |
MDFIC |
NNNGGCATTAAGAGATCAAC |
NGG |
2 |
0.3705 |
Tier II |
| 6 |
NC_000001.11 |
1 |
76420421 |
+ |
ST6GALNAC3 |
NNNGTCCTTAAGAATTCAAC |
NGG |
2 |
0.3281 |
Tier II |
| 7 |
NC_000008.11 |
8 |
62341210 |
+ |
NKAIN3 |
NNNGTAATTATGAGTTCAAC |
NGG |
2 |
0.2857 |
Tier II |
| 8 |
NC_000020.11 |
20 |
37818182 |
- |
CTNNBL1 |
NNNATCATTAAGTGTTCAAC |
NGG |
2 |
0.27 |
Tier II |
| 9 |
NC_000008.11 |
8 |
69673716 |
+ |
SLCO5A1 |
NNNGTCATTAAGTATTCAAC |
NGG |
2 |
0.225 |
Tier II |
| 10 |
NC_000017.11 |
17 |
19976059 |
+ |
AKAP10 |
NNNGTCATTCAGAGTTCAAT |
NGG |
2 |
0.1 |
Tier II |
| 11 |
NC_000011.10 |
11 |
66957031 |
+ |
PC |
NNNGTGATTAAGAGTTCAAC |
NGT |
2 |
0.0081 |
Tier II |
| 12 |
NC_000010.11 |
10 |
17963526 |
+ |
SLC39A12 |
NNNGTCATTAAGAGTTCAAC |
NCC |
2 |
0.0 |
Tier II |
| 13 |
NC_000002.12 |
2 |
111197863 |
- |
MIR4435-2HG |
NNNGTCATTTGGAGTTCAAC |
NGG |
2 |
0.5735 |
Tier III |
| 14 |
NC_000003.12 |
3 |
139473239 |
- |
COPB2-DT |
NNNATCATTAAGAGTTCAAA |
NGG |
2 |
0.45 |
Tier III |
| 15 |
NC_000012.12 |
12 |
69737942 |
- |
LOC101928002 |
NNNGTCATTAAGAGTTTAAA |
NGG |
2 |
0.2333 |
Tier III |
| 16 |
NC_000007.14 |
7 |
26428040 |
+ |
LINC02981 |
NNNGGCATTAAGAGTTCACC |
NGG |
2 |
0.1324 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
119784556 |
+ |
Erc1 |
NNNGCAATTAAGAGTTCAAC |
NGG |
2 |
0.9286 |
Tier II |
| 2 |
NC_000077.6 |
11 |
32016891 |
- |
Nsg2 |
NNNGTAACTAAGAGTTCAAC |
NGG |
2 |
0.9286 |
Tier II |
| 3 |
NC_000069.6 |
3 |
84237454 |
- |
Trim2 |
NNNGTCATTAAGGGTTCAAC |
NGG |
1 |
0.6522 |
Tier II |
| 4 |
NC_000073.6 |
7 |
67317134 |
- |
Mef2a |
NNNGTCATTAAGATTTCAAC |
NGG |
1 |
0.2667 |
Tier II |
| 5 |
NC_000067.6 |
1 |
60370027 |
+ |
Cyp20a1 |
NNNGTGATTAAGAGTTCAGC |
NGG |
2 |
0.1875 |
Tier II |
| 6 |
NC_000080.6 |
14 |
121924145 |
+ |
Ubac2 |
NNNGTCATTAAGACTTCCAC |
NGG |
2 |
0.0816 |
Tier II |
| 7 |
NC_000068.7 |
2 |
49809626 |
- |
Lypd6b |
NNNGTCATTAAGATTTCAAC |
NTG |
2 |
0.0104 |
Tier II |
| 8 |
NC_000069.6 |
3 |
84237454 |
- |
Gm40078 |
NNNGTCATTAAGGGTTCAAC |
NGG |
1 |
0.6522 |
Tier III |
| 9 |
NC_000076.6 |
10 |
116725384 |
- |
1700030O20Rik |
NNNGTCATAAATAGTTCAAC |
NGG |
2 |
0.3571 |
Tier III |
| 10 |
NC_000070.6 |
4 |
70684040 |
- |
Gm42297 |
NNNTTCATTAAGAGTTCAAA |
NGG |
2 |
0.1818 |
Tier III |
| 11 |
NC_000084.6 |
18 |
41756640 |
+ |
Gm50410 |
NNNGTCATTAAGAGTTCTAT |
NGG |
2 |
0.15 |
Tier III |
| 12 |
NC_000080.6 |
14 |
121924145 |
+ |
Gm46485 |
NNNGTCATTAAGACTTCCAC |
NGG |
2 |
0.0816 |
Tier III |
| 13 |
NC_000074.6 |
8 |
80370049 |
- |
Gm30605 |
NNNGTCATTAAGACTTGAAC |
NGG |
2 |
0.0252 |
Tier III |
Other clones with same target sequence:
(none)
