Construct: sgRNA BRDN0001146090

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GATGGTAGTTAATGAGCTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BTK (695)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77073

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 101358342 + BTK NNNGGTAGTTAATGAGCTCA NGG 0 1.0 Tier I
2 NC_000023.11 X 101357231 + BTK NNNGGTAGTTAATGAACTCA NGG 1 1.0 Tier II
3 NC_000023.11 X 77451330 + FGF16 NNNGCTACTTAATGAGCTCA NGG 2 0.4835 Tier II
4 NC_000011.10 11 107446904 - CWF19L2 NNNGATAGTTAATGAGTTCA NGG 2 0.4044 Tier II
5 NC_000022.11 22 27918099 + PITPNB NNNGGTAGTAAATGAGATCA NGG 2 0.4 Tier II
6 NC_000009.12 9 124707864 - NR6A1 NNNGGTAGTGAAAGAGCTCA NGG 2 0.3462 Tier II
7 NC_000004.12 4 92339616 + GRID2 NNNGGTAATTAATTAGCTCA NGG 2 0.2667 Tier II
8 NC_000003.12 3 140468536 - CLSTN2 NNNGGTAGTCAATGAGATCA NGG 2 0.2489 Tier II
9 NC_000002.12 2 115649265 - DPP10 NNNGGTTGTTAATGAGCTCA NAG 2 0.1134 Tier II
10 NC_000003.12 3 24854244 + RARB NNNGGTAGTAAATGAGCTGA NGG 2 0.1071 Tier II
11 NC_000007.14 7 152262510 + KMT2C NNNGTTAGTTAATGAGCTCA NCG 2 0.0321 Tier II
12 NC_000014.9 14 74023170 - BBOF1 NNNGGTAGTTAATGAGGCCA NGG 2 0.0252 Tier II
13 NC_000003.12 3 185085955 + C3orf70 NNNGGTAGTTACTGAGCTCA NGA 2 0.0183 Tier II
14 NC_000003.12 3 140468536 - LOC105374132 NNNGGTAGTCAATGAGATCA NGG 2 0.2489 Tier III
15 NC_000007.14 7 38032176 - LOC105375236 NNNGGTTGTTCATGAGCTCA NGG 2 0.175 Tier III
16 NC_000004.12 4 96344939 - LINC02267 NNNGGTATTTAAGGAGCTCA NGG 2 0.163 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 134554431 + Btk NNNGGTAGTTAATGAGCTCA NGG 0 1.0 Tier I
2 NC_000073.6 7 141107484 + Ano9 NNNGAGAGTTAATGAGCTCA NGG 2 0.4952 Tier I
3 NC_000068.7 2 9937168 - Taf3 NNNGGTAATTAATGAGCACA NGG 2 0.6667 Tier II
4 NC_000083.6 17 90569203 + Nrxn1 NNNGGTAATTAATGAGTTCA NGG 2 0.4667 Tier II
5 NC_000074.6 8 127516749 + Pard3 NNNGGTAGATAATGAGCCCA NGG 2 0.398 Tier II
6 NC_000083.6 17 89084675 + Fshr NNNGGTGGTCAATGAGCTCA NGG 2 0.3765 Tier II
7 NC_000071.6 5 19749764 - Magi2 NNNGGTAGTTCATGAGCACA NGG 2 0.2667 Tier II
8 NC_000079.6 13 13392519 - Gpr137b NNNCGTAGTTAATGAGCTAA NGG 2 0.2269 Tier II
9 NC_000071.6 5 136489301 + Cux1 NNNGGTAGATAATGAGCTCC NGG 2 0.211 Tier II
10 NC_000070.6 4 84410638 - Bnc2 NNNGTTAGTTGATGAGCTCA NGG 2 0.195 Tier II
11 NC_000072.6 6 83388311 - Tet3 NNNGGTGGTTAATGAGCTCA NAG 2 0.183 Tier II
12 NC_000075.6 9 55965338 + 4930563M21Rik NNNGGTGGTTAATGAGCTCA NAG 2 0.183 Tier II
13 NC_000079.6 13 100473907 - Gtf2h2 NNNGCTAGTTAATGAGCTCC NGG 2 0.1786 Tier II
14 NC_000073.6 7 80862585 - Zscan2 NNNGGTAGTTCATGAGCCCA NGG 2 0.1714 Tier II
15 NC_000083.6 17 56308521 - Uhrf1 NNNGGTAGTTACTGAGCCCA NGG 2 0.1128 Tier II
16 NC_000072.6 6 87517818 + Arhgap25 NNNTGTAGTTAATGAGCTCC NGG 2 0.0826 Tier II
17 NC_000080.6 14 20148779 + Kcnk5 NNNGGTAGTCAATGAGCTCA NGA 2 0.037 Tier II
18 NC_000074.6 8 66222652 + Marchf1 NNNGGTACTTAATGAGCTCA NTG 2 0.024 Tier II
19 NC_000067.6 1 9941249 - Mcmdc2 NNNGGTAATTAATGAGCTCA NGC 2 0.0222 Tier II
20 NC_000076.6 10 127695585 + Nemp1 NNNGGTAGTTAATGACCTCA NGT 2 0.0 Tier II
21 NC_000084.6 18 64225368 - St8sia3os NNNGGTAGTTAATGAGCACA NGG 1 0.6667 Tier III
22 NC_000079.6 13 12649310 - Gpr137b-ps NNNCGTAGTTAATGAGCTAA NGG 2 0.2269 Tier III
23 NC_000068.7 2 138707721 + Gm14061 NNNGGTAGTTGATTAGCTCA NGG 2 0.1733 Tier III
24 NC_000070.6 4 22943627 - Gm30886 NNNTGTAGTTCATGAGCTCA NGG 2 0.1455 Tier III
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Other clones with same target sequence:

(none)