Construct: sgRNA BRDN0001146127

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGGCACAAGGTCCTACATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP2K1 (5604)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76213

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 66481875 + MAP2K1 NNNCACAAGGTCCTACATGT NGG 0 1.0 Tier I
2 NC_000001.11 1 41959767 - HIVEP3 NNNCATAAGGTACTACATGT NGG 2 0.6633 Tier II
3 NC_000001.11 1 226648055 + ITPKB NNNCAGAGGGTCCTACATGT NGG 2 0.3667 Tier II
4 NC_000013.11 13 107415897 - NALF1 NNNCAGAAGGTCTTACATGT NGG 2 0.35 Tier II
5 NC_000011.10 11 71155954 + SHANK2 NNNCACAAGGTCTTACAGGT NGG 2 0.2333 Tier II
6 NC_000007.14 7 71472805 - GALNT17 NNNCACAAGGTCCTACATTT NAG 2 0.1728 Tier II
7 NC_000003.12 3 192175017 - FGF12 NNNCACAAGGTCCTAGATGT NGG 1 0.1538 Tier II
8 NC_000007.14 7 146509088 + CNTNAP2 NNNCACAAGGTCCCTCATGT NGG 2 0.0571 Tier II
9 NC_000001.11 1 216324754 + USH2A NNNCATAAGGTCCTACATGT NGT 2 0.015 Tier II
10 NC_000003.12 3 126713434 + CHCHD6 NNNCACAAGGTCCTTCATGT NTG 2 0.0078 Tier II
11 NC_000017.11 17 45202177 + FMNL1-DT NNNCACAGGGACCTACATGT NGG 2 0.55 Tier III
12 NC_000001.11 1 116478425 + LINC01762 NNNCATAAGGTTCTACATGT NGG 2 0.5 Tier III
13 NC_000002.12 2 146874872 + LINC01911 NNNCACAAGATCCTACATGG NGG 2 0.1647 Tier III
14 NC_000023.11 X 140761978 - LINC00632 NNNCTCAAGGTCCTACAGGT NGG 2 0.1212 Tier III
15 NC_000005.10 5 101761400 + LOC105379102 NNNCACAAGGTCCCTCATGT NGG 2 0.0571 Tier III
16 NC_000006.12 6 23372884 - LOC102724749 NNNCACAAGGTCCTCCATGT NGT 2 0.0044 Tier III
17 NC_000006.12 6 23372884 - LOC105374976 NNNCACAAGGTCCTCCATGT NGT 2 0.0044 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 64193707 - Map2k1 NNNCACGAGATCCTACATGT NGG 2 0.6588 Tier I
2 NC_000082.6 16 38752314 + B4galt4 NNNCACAAGGTCATACATTT NGG 2 0.2564 Tier I
3 NC_000080.6 14 30886554 - Itih4 NNNCACAAGGTTCCACATGT NGG 2 0.1538 Tier I
4 NC_000068.7 2 164772226 - Ube2c NNNGACAAGGTCCTACCTGT NGG 2 0.0882 Tier I
5 NC_000076.6 10 71097389 - Bicc1 NNNAACAAGGTCCAACATGT NGG 2 0.5213 Tier II
6 NC_000085.6 19 48475672 + Sorcs3 NNNCACAAGGCCCTGCATGT NGG 2 0.4333 Tier II
7 NC_000071.6 5 31413497 + Gm36182 NNNCACAAGGTCCCAAATGT NGG 2 0.2857 Tier II
8 NC_000075.6 9 6221317 + Pdgfd NNNCACAAGGTCCCAAATGT NGG 2 0.2857 Tier II
9 NC_000085.6 19 22758178 - Trpm3 NNNCACAGGGTCCTCCATGT NGG 2 0.2 Tier II
10 NC_000076.6 10 119882492 + Grip1 NNNCATAAGGTCCTACGTGT NGG 2 0.1639 Tier II
11 NC_000067.6 1 32344990 + Khdrbs2 NNNCACAAGGTCCTCCATGT NAG 2 0.0707 Tier II
12 NC_000068.7 2 65211536 - Cobll1 NNNCACAAGGTCCTACATTC NGG 2 0.0606 Tier II
13 NC_000071.6 5 13598635 - Sema3a NNNCACAAGGTCCTGCATGT NGC 2 0.0144 Tier II
14 NC_000069.6 3 153101100 - Gm52620 NNNCACAAGGGCCTACATGT NGG 1 0.4 Tier III
15 NC_000080.6 14 48774493 - Otx2os1 NNNCACAAGGTTTTACATGT NGG 2 0.3769 Tier III
16 NC_000071.6 5 119636036 + Tbx3os1 NNNCCCAAGGTCCTACATCT NGG 2 0.2241 Tier III
17 NC_000081.6 15 80438035 - Gm31369 NNNCACAAGGGCCTACATCT NGG 2 0.1793 Tier III
18 NC_000074.6 8 10866137 + Gm32540 NNNCACAAGGTCCAACATGT NAG 2 0.1605 Tier III
19 NC_000076.6 10 67573682 - Gm32364 NNNCACAATGTCCTTCATGT NGG 2 0.1067 Tier III
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Other clones with same target sequence:

(none)