Construct: sgRNA BRDN0001146186
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGATGATGCTATATGTGAA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- NME7 (29922)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000001.11 | 1 | 169298694 | - | NME7 | NNNATGATGCTATATGTGAA | NGG | 0 | 1.0 | Tier I |
2 | NC_000009.12 | 9 | 116187374 | + | PAPPA | NNNATGAAGCTCTATGTGAA | NGG | 2 | 0.2105 | Tier I |
3 | NC_000006.12 | 6 | 88066321 | + | SPACA1 | NNNATGATGCTTTAAGTGAA | NGG | 2 | 0.193 | Tier I |
4 | NC_000018.10 | 18 | 72809903 | - | NETO1 | NNNATGATGCTATATATTAA | NGG | 2 | 0.6667 | Tier II |
5 | NC_000020.11 | 20 | 13349680 | - | TASP1 | NNNATCATGTTATATGTGAA | NGG | 2 | 0.6417 | Tier II |
6 | NC_000011.10 | 11 | 7025568 | + | NLRP14 | NNNATTATGCTAAATGTGAA | NGG | 2 | 0.4615 | Tier II |
7 | NC_000002.12 | 2 | 170229755 | - | MYO3B | NNNATTATACTATATGTGAA | NGG | 2 | 0.4286 | Tier II |
8 | NC_000015.10 | 15 | 88827391 | - | ACAN | NNNATGATGCTGTAAGTGAA | NGG | 2 | 0.4181 | Tier II |
9 | NC_000001.11 | 1 | 66162354 | - | PDE4B | NNNTAGATGCTATATGTGAA | NGG | 2 | 0.3182 | Tier II |
10 | NC_000012.12 | 12 | 463881 | + | B4GALNT3 | NNNATGATGCTGTATGTGGA | NGG | 2 | 0.2708 | Tier II |
11 | NC_000014.9 | 14 | 29744901 | - | PRKD1 | NNNATGATTCTATGTGTGAA | NGG | 2 | 0.2489 | Tier II |
12 | NC_000009.12 | 9 | 112570315 | + | KIAA1958 | NNNATGGTGCTATACGTGAA | NGG | 2 | 0.1925 | Tier II |
13 | NC_000006.12 | 6 | 65295597 | - | EYS | NNNATGATGGTATATGTCAA | NGG | 2 | 0.1852 | Tier II |
14 | NC_000011.10 | 11 | 19614507 | - | NAV2 | NNNAGGATGCTATATGTGCA | NGG | 2 | 0.1324 | Tier II |
15 | NC_000007.14 | 7 | 117187064 | + | ST7 | NNNAAGATGCTCTATGTGAA | NGG | 2 | 0.1316 | Tier II |
16 | NC_000001.11 | 1 | 39323903 | + | MACF1 | NNNAAGATGCTATATGTGAA | NAG | 2 | 0.1296 | Tier II |
17 | NC_000011.10 | 11 | 121489681 | + | SORL1 | NNNATGCTGCTATCTGTGAA | NGG | 2 | 0.0938 | Tier II |
18 | NC_000002.12 | 2 | 178705162 | + | TTN | NNNATGATGCTATATATGAA | NGA | 2 | 0.0694 | Tier II |
19 | NC_000004.12 | 4 | 152908711 | - | ARFIP1 | NNNATGATGTTATATGTGAA | NGA | 2 | 0.0654 | Tier II |
20 | NC_000023.11 | X | 151662080 | - | PASD1 | NNNATGATGCTAAATGTGAA | NGA | 2 | 0.0481 | Tier II |
21 | NC_000016.10 | 16 | 76493269 | + | CNTNAP4 | NNNATGATGCCATATGTGAA | NGA | 2 | 0.0463 | Tier II |
22 | NC_000003.12 | 3 | 160784301 | - | PPM1L | NNNATGATACTATATGTGAA | NGA | 2 | 0.0446 | Tier II |
23 | NC_000007.14 | 7 | 11403660 | - | THSD7A | NNNATGATGCTAAATGTGAA | NTG | 2 | 0.027 | Tier II |
24 | NC_000002.12 | 2 | 29683735 | + | ALK | NNNATGATGTTATATGTGAA | NGC | 2 | 0.0209 | Tier II |
25 | NC_000007.14 | 7 | 128981668 | + | TNPO3 | NNNATGATGATATATGTGAA | NGT | 2 | 0.014 | Tier II |
26 | NC_000002.12 | 2 | 114454210 | + | DPP10 | NNNATGGTGCTATATGTGAA | NGT | 2 | 0.0114 | Tier II |
27 | NC_000005.10 | 5 | 95600786 | + | ARSK | NNNATGATGCTATTTGTGAA | NGT | 2 | 0.0086 | Tier II |
28 | NC_000007.14 | 7 | 7670608 | - | RPA3 | NNNATGATGCTATATCTGAA | NGG | 1 | 0.0 | Tier II |
29 | NC_000007.14 | 7 | 7670608 | - | UMAD1 | NNNATGATGCTATATCTGAA | NGG | 1 | 0.0 | Tier II |
30 | NC_000020.11 | 20 | 13349680 | - | LOC124904872 | NNNATCATGTTATATGTGAA | NGG | 2 | 0.6417 | Tier III |
31 | NC_000010.11 | 10 | 121277513 | - | LOC105378523 | NNNATCATGCTACATGTGAA | NGG | 2 | 0.5383 | Tier III |
32 | NC_000001.11 | 1 | 203390473 | + | LOC102723529 | NNNATGATGCAATATGAGAA | NGG | 2 | 0.4 | Tier III |
33 | NC_000006.12 | 6 | 134733585 | + | LOC101928277 | NNNATGGTGCTATGTGTGAA | NGG | 2 | 0.3294 | Tier III |
34 | NC_000007.14 | 7 | 117187064 | + | ST7-OT3 | NNNAAGATGCTCTATGTGAA | NGG | 2 | 0.1316 | Tier III |
35 | NC_000002.12 | 2 | 178705162 | + | LOC124906100 | NNNATGATGCTATATATGAA | NGA | 2 | 0.0694 | Tier III |
36 | NC_000002.12 | 2 | 178705162 | + | LOC124907912 | NNNATGATGCTATATATGAA | NGA | 2 | 0.0694 | Tier III |
37 | NC_000004.12 | 4 | 19494453 | - | LOC105374511 | NNNATGATGCTGTATGTGAA | NGA | 2 | 0.0502 | Tier III |
38 | NC_000006.12 | 6 | 84362378 | - | LOC107986620 | NNNATGATGCAATATTTGAA | NGG | 2 | 0.0 | Tier III |
39 | NC_000006.12 | 6 | 84362378 | - | LOC107986621 | NNNATGATGCAATATTTGAA | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000067.6 | 1 | 24562708 | - | Col19a1 | NNNATGAAGCTATATGTGAA | NGG | 1 | 0.8 | Tier II |
2 | NC_000084.6 | 18 | 7508187 | + | Mpp7 | NNNATGATGTTACATGTGAA | NGG | 2 | 0.743 | Tier II |
3 | NC_000078.6 | 12 | 36160904 | - | Ankmy2 | NNNATGATGATGTATGTGAA | NGG | 2 | 0.6259 | Tier II |
4 | NC_000077.6 | 11 | 89661909 | + | Ankfn1 | NNNATGATAATATATGTGAA | NGG | 2 | 0.5571 | Tier II |
5 | NC_000069.6 | 3 | 37511557 | - | Spata5 | NNNATGGTGCTATATGCGAA | NGG | 2 | 0.4983 | Tier II |
6 | NC_000071.6 | 5 | 150729657 | + | Pds5b | NNNATCATGCTGTATGTGAA | NGG | 2 | 0.4924 | Tier II |
7 | NC_000074.6 | 8 | 13612056 | + | Rasa3 | NNNATGAAGCTAGATGTGAA | NGG | 2 | 0.2087 | Tier II |
8 | NC_000072.6 | 6 | 87836522 | + | Isy1 | NNNATGATGGTATTTGTGAA | NGG | 2 | 0.2074 | Tier II |
9 | NC_000067.6 | 1 | 62065895 | - | Pard3b | NNNATGATGCTACATGTGAA | NAG | 2 | 0.2047 | Tier II |
10 | NC_000073.6 | 7 | 120853238 | - | Eef2k | NNNATGAAGCTATATGTGAA | NCG | 2 | 0.0857 | Tier II |
11 | NC_000079.6 | 13 | 16981938 | - | Sugct | NNNTTGATGCTATATGTGAA | NGA | 2 | 0.0442 | Tier II |
12 | NC_000086.7 | X | 42517916 | - | Sh2d1a | NNNATGATGTTATATGTGAA | NTG | 2 | 0.0367 | Tier II |
13 | NC_000082.6 | 16 | 6405725 | - | Rbfox1 | NNNATGATGCTATATGTGAT | NGC | 2 | 0.0133 | Tier II |
14 | NC_000080.6 | 14 | 75971619 | - | Gtf2f2 | NNNATGATTCTATATGTGAA | NGT | 2 | 0.0086 | Tier II |
15 | NC_000071.6 | 5 | 103212186 | - | Mapk10 | NNNATGATGCTATATGTGAA | NTA | 2 | 0.0 | Tier II |
16 | NC_000081.6 | 15 | 66551875 | - | Tmem71 | NNNATGATGCTATGTCTGAA | NGG | 2 | 0.0 | Tier II |
17 | NC_000074.6 | 8 | 48871208 | + | Tenm3 | NNNATGATGCTATTTTTGAA | NGG | 2 | 0.0 | Tier II |
18 | NC_000077.6 | 11 | 103883164 | + | Nsf | NNNATGCTGCTATATTTGAA | NGG | 2 | 0.0 | Tier II |
19 | NC_000068.7 | 2 | 70932067 | - | Gm39843 | NNNATGATGCTATATCTCAA | NGG | 2 | 0.0 | Tier III |
20 | NC_000074.6 | 8 | 48871208 | + | Gm19744 | NNNATGATGCTATTTTTGAA | NGG | 2 | 0.0 | Tier III |