Construct: sgRNA BRDN0001146365
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTTGATACCATGATCTTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GUCY2C (2984)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77565
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
14651445 |
- |
GUCY2C |
NNNTGATACCATGATCTTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
13608728 |
+ |
GRIN2B |
NNNTGACACCATGATCTTGG |
NGG |
2 |
0.0859 |
Tier I |
| 3 |
NC_000010.11 |
10 |
122039303 |
+ |
TACC2 |
NNNTGAAACCATGATCTTCT |
NGG |
2 |
0.6125 |
Tier II |
| 4 |
NC_000002.12 |
2 |
195691030 |
- |
SLC39A10 |
NNNTGATACAATGAACTTCG |
NGG |
2 |
0.5018 |
Tier II |
| 5 |
NC_000008.11 |
8 |
132391245 |
- |
KCNQ3 |
NNNTGATACCATCATCTTCA |
NGG |
2 |
0.3947 |
Tier II |
| 6 |
NC_000002.12 |
2 |
170583359 |
- |
MYO3B |
NNNTGATGCCATGTTCTTCG |
NGG |
2 |
0.3911 |
Tier II |
| 7 |
NC_000007.14 |
7 |
75920678 |
+ |
POR |
NNNTGATACTTTGATCTTCG |
NGG |
2 |
0.2896 |
Tier II |
| 8 |
NC_000014.9 |
14 |
68243945 |
- |
RAD51B |
NNNTGATACCATAATCTTGG |
NGG |
2 |
0.1154 |
Tier II |
| 9 |
NC_000003.12 |
3 |
130479092 |
- |
COL6A5 |
NNNTAATACCATGATCTTGG |
NGG |
2 |
0.1083 |
Tier II |
| 10 |
NC_000004.12 |
4 |
51895121 |
+ |
DCUN1D4 |
NNNTGATACCATCATCTTGG |
NGG |
2 |
0.0526 |
Tier II |
| 11 |
NC_000023.11 |
X |
6177832 |
- |
NLGN4X |
NNNTGATACCATCATCTTGG |
NGG |
2 |
0.0526 |
Tier II |
| 12 |
NC_000009.12 |
9 |
23696054 |
+ |
ELAVL2 |
NNNTGATACCATGATCTTCA |
NTG |
2 |
0.0365 |
Tier II |
| 13 |
NC_000002.12 |
2 |
199467938 |
- |
SATB2 |
NNNTGATACCATGATGTTGG |
NGG |
2 |
0.0192 |
Tier II |
| 14 |
NC_000001.11 |
1 |
235375687 |
- |
TBCE |
NNNTGATACCATGATCTTCC |
NTG |
2 |
0.0167 |
Tier II |
| 15 |
NC_000005.10 |
5 |
22316920 |
- |
CDH12 |
NNNTGATACCATGATCTTAG |
NGT |
2 |
0.0069 |
Tier II |
| 16 |
NC_000014.9 |
14 |
53712928 |
- |
LINC02331 |
NNNTGATACAATGAACTTCG |
NGG |
2 |
0.5018 |
Tier III |
| 17 |
NC_000014.9 |
14 |
53712928 |
- |
LOC105370504 |
NNNTGATACAATGAACTTCG |
NGG |
2 |
0.5018 |
Tier III |
| 18 |
NC_000020.11 |
20 |
30414588 |
- |
FRG1BP |
NNNTGATACAATGGTCTTCG |
NGG |
2 |
0.4044 |
Tier III |
| 19 |
NT_167213.1 |
|
61786 |
+ |
LOC283788 |
NNNTGATACAATGGTCTTCG |
NGG |
2 |
0.4044 |
Tier III |
| 20 |
NC_000010.11 |
10 |
106141000 |
- |
LINC02624 |
NNNTAATACCATCATCTTCG |
NGG |
2 |
0.3649 |
Tier III |
| 21 |
NC_000007.14 |
7 |
17539022 |
+ |
LINC02889 |
NNNTGATACCATGAACTTTG |
NGG |
2 |
0.2672 |
Tier III |
| 22 |
NC_000004.12 |
4 |
177787677 |
+ |
LINC01098 |
NNNTGATACCATGACCTTAG |
NGG |
2 |
0.1169 |
Tier III |
| 23 |
NC_000012.12 |
12 |
13608728 |
+ |
LOC105369668 |
NNNTGACACCATGATCTTGG |
NGG |
2 |
0.0859 |
Tier III |
| 24 |
NC_000002.12 |
2 |
144844014 |
+ |
TEX41 |
NNNTGATACCATGATGTTTG |
NGG |
2 |
0.071 |
Tier III |
| 25 |
NC_000002.12 |
2 |
95021525 |
- |
MAL-AS1 |
NNNTGATACCATCATCTTGG |
NGG |
2 |
0.0526 |
Tier III |
| 26 |
NT_187510.1 |
|
23612 |
+ |
LOC105379565 |
NNNTGATACCATGATCTTTG |
NTG |
2 |
0.018 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
135772318 |
+ |
Grin2b |
NNNTGACACCATGATCTTGG |
NGG |
2 |
0.0859 |
Tier I |
| 2 |
NC_000086.7 |
X |
134314121 |
- |
Cenpi |
NNNTGAGACCATGATCTTCA |
NGG |
2 |
0.5515 |
Tier II |
| 3 |
NC_000074.6 |
8 |
88204326 |
+ |
Tent4b |
NNNTGATACTATGATCTTCC |
NGG |
2 |
0.4034 |
Tier II |
| 4 |
NC_000076.6 |
10 |
30713991 |
- |
Ncoa7 |
NNNTAATACCATGATCTTTG |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000068.7 |
2 |
125312156 |
- |
Fbn1 |
NNNTCATACCATGATCTTAG |
NGG |
2 |
0.3367 |
Tier II |
| 6 |
NC_000085.6 |
19 |
16254481 |
+ |
Gnaq |
NNNTGATACCATGATGTTCC |
NGG |
2 |
0.0659 |
Tier II |
| 7 |
NC_000081.6 |
15 |
58252852 |
+ |
Gm36193 |
NNNTGATACCATGTTCTTAG |
NGG |
2 |
0.2286 |
Tier III |
| 8 |
NC_000073.6 |
7 |
48763046 |
+ |
Gm32263 |
NNNAGATACCATGACCTTCG |
NGG |
2 |
0.1299 |
Tier III |
Other clones with same target sequence:
(none)
