Construct: sgRNA BRDN0001146456

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCTTTCCAGATGCTAAGAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MLKL (197259)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77540

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 74695303 - MLKL NNNTTCCAGATGCTAAGAAG NGG 0 1.0 Tier I
2 NC_000003.12 3 15582893 + HACL1 NNNTTCCCGATGATAAGAAG NGG 2 0.1648 Tier I
3 NC_000008.11 8 25368731 + DOCK5 NNNTTCCATATGGTAAGAAG NGG 2 0.0727 Tier I
4 NC_000007.14 7 151814460 + PRKAG2 NNNTTCCAGATGCTAATAAG NAG 2 0.0648 Tier I
5 NC_000015.10 15 65938496 - MEGF11 NNNTTCCAGGTGCTAAGAAA NGG 2 0.5208 Tier II
6 NC_000016.10 16 55850393 - CES5A NNNTTCCAGAAGCTGAGAAG NGG 2 0.4875 Tier II
7 NC_000003.12 3 195146676 - XXYLT1 NNNTTCAAAATGCTAAGAAG NGG 2 0.4821 Tier II
8 NC_000007.14 7 94990717 - PPP1R9A NNNTACTAGATGCTAAGAAG NGG 2 0.4063 Tier II
9 NC_000011.10 11 27695819 - BDNF NNNTACTAGATGCTAAGAAG NGG 2 0.4063 Tier II
10 NC_000002.12 2 25650198 - DTNB NNNTTCCTGATACTAAGAAG NGG 2 0.4 Tier II
11 NC_000006.12 6 4713404 + CDYL NNNTTCCAGATGCTAAGAGA NGG 2 0.3516 Tier II
12 NC_000019.10 19 13053409 + NFIX NNNTTCCAGATGCCAAGAAG NGG 1 0.2857 Tier II
13 NC_000022.11 22 48685867 + TAFA5 NNNTTCCAGAAGCTAAGAGG NGG 2 0.2813 Tier II
14 NC_000011.10 11 33039596 + TCP11L1 NNNTTCCTGATGCTGAGAAG NGG 2 0.2786 Tier II
15 NC_000023.11 X 135581751 + INTS6L NNNTTCCAGATGCTAAGAAG NAG 1 0.2593 Tier II
16 NC_000005.10 5 110422712 - TMEM232 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
17 NC_000014.9 14 93111414 - ITPK1 NNNTTCTAGATGCTCAGAAG NGG 2 0.2216 Tier II
18 NC_000004.12 4 172869881 - GALNTL6 NNNTTCCAGAGCCTAAGAAG NGG 2 0.2118 Tier II
19 NC_000002.12 2 153707677 - GALNT13 NNNTTGCAGATGATAAGAAG NGG 2 0.1923 Tier II
20 NC_000023.11 X 129981216 - BCORL1 NNNATCCAGATGCTAAGAGG NGG 2 0.1786 Tier II
21 NC_000001.11 1 45058384 - ZSWIM5 NNNTTCCAGATGTTAACAAG NGG 2 0.1647 Tier II
22 NC_000012.12 12 103793845 - NT5DC3 NNNTTCCAGGTGCTAAGCAG NGG 2 0.1058 Tier II
23 NC_000003.12 3 171114179 + TNIK NNNTTCCAGATGCTGAGAAG NGA 2 0.0451 Tier II
24 NC_000005.10 5 19567794 - CDH18 NNNTTCCAAATGCTAAGAAG NGA 2 0.0446 Tier II
25 NC_000006.12 6 38353230 - BTBD9 NNNTTCCAGATGCTAAGAAG NCT 2 0.0 Tier II
26 NC_000012.12 12 62868255 - PPM1H NNNTTCCAGATGCTATTAAG NGG 2 0.0 Tier II
27 NC_000018.10 18 38722025 - LOC105372076 NNNTTCAAGATGCTAAGAAG NGG 1 0.75 Tier III
28 NC_000009.12 9 26071446 - LINC03106 NNNTGCCAGATGCTAAGAAG NGG 1 0.64 Tier III
29 NC_000011.10 11 30249872 - ARL14EP-DT NNNTTTCAGATGCTGAGAAG NGG 2 0.6036 Tier III
30 NC_000010.11 10 23055175 + LOC107984215 NNNTTCTGGATGCTAAGAAG NGG 2 0.5958 Tier III
31 NC_000004.12 4 34146708 + LINC02484 NNNTTCCAGGAGCTAAGAAG NGG 2 0.4167 Tier III
32 NC_000011.10 11 27695819 - BDNF-AS NNNTACTAGATGCTAAGAAG NGG 2 0.4063 Tier III
33 NC_000014.9 14 99001580 + LOC107984696 NNNTCCCAGATGATAAGAAG NGG 2 0.3846 Tier III
34 NC_000002.12 2 157914811 - LOC105373714 NNNTACCAGAAGCTAAGAAG NGG 2 0.375 Tier III
35 NC_000008.11 8 129515718 - CCDC26 NNNTACCAGAAGCTAAGAAG NGG 2 0.375 Tier III
36 NC_000011.10 11 34717094 - LOC102723568 NNNTACCAGAAGCTAAGAAG NGG 2 0.375 Tier III
37 NC_000014.9 14 83912091 + LINC02305 NNNTACCAGAAGCTAAGAAG NGG 2 0.375 Tier III
38 NC_000006.12 6 81863689 + LINC02542 NNNTTCAAGATGCTAAGTAG NGG 2 0.375 Tier III
39 NC_000010.11 10 120932952 + LOC105378519 NNNCTCCAGAGGCTAAGAAG NGG 2 0.2588 Tier III
40 NC_000023.11 X 128361393 - LOC107985698 NNNTTCCAGAGGCTCAGAAG NGG 2 0.1091 Tier III
41 NC_000014.9 14 24366168 + LOC124903291 NNNTTCCAGATGCTAGGAGG NGG 2 0.0721 Tier III
42 NC_000003.12 3 171114179 + LOC105374216 NNNTTCCAGATGCTGAGAAG NGA 2 0.0451 Tier III
43 NC_000013.11 13 60265700 + LINC00434 NNNTTCCACATGCTAAGAAG NTG 2 0.021 Tier III
44 NC_000016.10 16 52581263 + CASC16 NNNTTCCAGATGCTAGGAAG NCG 2 0.0206 Tier III
45 NC_000016.10 16 52581263 + LOC105371265 NNNTTCCAGATGCTAGGAAG NCG 2 0.0206 Tier III
46 NC_000023.11 X 141623637 - SPANXA2-OT1 NNNTTCCAGATGCTGAGAAG NGC 2 0.0144 Tier III
47 NC_000012.12 12 62868255 - LOC105369795 NNNTTCCAGATGCTATTAAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 80103206 - Idh2 NNNTTCTAGATGCTGAGAAG NGG 2 0.5281 Tier I
2 NC_000080.6 14 67786268 - Dock5 NNNTTCCACATGGTAAGAAG NGG 2 0.0734 Tier I
3 NC_000071.6 5 127417981 - Tmem132c NNNTCCTAGATGCTAAGAAG NGG 2 0.8125 Tier II
4 NC_000067.6 1 87533887 - Ngef NNNTTCAAGATGCTAAGAAA NGG 2 0.7031 Tier II
5 NC_000077.6 11 76729006 + Gosr1 NNNTTACAGATGCTGAGAAG NGG 2 0.6036 Tier II
6 NC_000075.6 9 67529491 + Tln2 NNNTCCCACATGCTAAGAAG NGG 2 0.5385 Tier II
7 NC_000075.6 9 117198817 + Rbms3 NNNTTCTAGATGCAAAGAAG NGG 2 0.503 Tier II
8 NC_000082.6 16 40046992 + Lsamp NNNTTCCAGATTCTAAGAAG NGG 1 0.3846 Tier II
9 NC_000075.6 9 63691271 - Smad3 NNNTTCAAGATGCTAAGAAC NGG 2 0.3214 Tier II
10 NC_000079.6 13 21447989 + Zscan26 NNNTTCAAGAGGCTAAGAAG NGG 2 0.3 Tier II
11 NC_000069.6 3 76471804 + Fstl5 NNNTTCCCAATGCTAAGAAG NGG 2 0.2755 Tier II
12 NC_000086.7 X 83681941 + Dmd NNNTTCCCAATGCTAAGAAG NGG 2 0.2755 Tier II
13 NC_000086.7 X 100681929 + Kif4 NNNTTCCCAATGCTAAGAAG NGG 2 0.2755 Tier II
14 NC_000067.6 1 11944141 + A830018L16Rik NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
15 NC_000067.6 1 54505035 + Pgap1 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
16 NC_000067.6 1 99834971 + Cntnap5b NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
17 NC_000068.7 2 41479513 - Lrp1b NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
18 NC_000069.6 3 15375170 - Sirpb1a NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
19 NC_000069.6 3 15499277 - Sirpb1b NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
20 NC_000069.6 3 15798662 - Sirpb1c NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
21 NC_000069.6 3 75097646 + Zbbx NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
22 NC_000070.6 4 121545315 - Gm12880 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
23 NC_000071.6 5 13559892 - Sema3a NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
24 NC_000072.6 6 61868859 + Ccser1 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
25 NC_000072.6 6 80141025 - Lrrtm4 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
26 NC_000072.6 6 106548384 - Cntn4 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
27 NC_000073.6 7 5409461 + Vmn1r58 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
28 NC_000073.6 7 14234053 - Sult2a6 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
29 NC_000073.6 7 100901186 - Arhgef17 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
30 NC_000074.6 8 16617393 + Csmd1 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
31 NC_000074.6 8 38055769 - Sgcz NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
32 NC_000075.6 9 20243974 + Olfr872 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
33 NC_000076.6 10 33158755 + Trdn NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
34 NC_000076.6 10 89984844 - Anks1b NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
35 NC_000078.6 12 89043867 - Nrxn3 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
36 NC_000078.6 12 118163092 - Dnah11 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
37 NC_000082.6 16 64543705 - Csnka2ip NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
38 NC_000083.6 17 58301134 - Cntnap5c NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
39 NC_000083.6 17 71548080 - Spdya NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
40 NC_000085.6 19 39446548 + Cyp2c38 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
41 NC_000085.6 19 39446548 + Cyp2c39 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
42 NC_000086.7 X 141286115 - Col4a6 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
43 NC_000086.7 X 144697347 - Rtl4 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier II
44 NC_000077.6 11 61153396 + Usp22 NNNTTCCAGTTGCTCAGAAG NGG 2 0.2406 Tier II
45 NC_000077.6 11 62064613 - Specc1 NNNTTCCAGATTCTAAGATG NGG 2 0.2071 Tier II
46 NC_000086.7 X 48342347 - Bcorl1 NNNATCCAGATGCTAAGAGG NGG 2 0.1786 Tier II
47 NC_000067.6 1 99876643 + Cntnap5b NNNTTCCCGATTCTAAGAAG NGG 2 0.1648 Tier II
48 NC_000073.6 7 102368317 + Stim1 NNNTTCCCGATTCTAAGAAG NGG 2 0.1648 Tier II
49 NC_000073.6 7 133982231 + Adam12 NNNTTCCCGATTCTAAGAAG NGG 2 0.1648 Tier II
50 NC_000076.6 10 22131326 - E030030I06Rik NNNTTCCCGATTCTAAGAAG NGG 2 0.1648 Tier II
51 NC_000076.6 10 102173181 + Mgat4c NNNTTCCCGATTCTAAGAAG NGG 2 0.1648 Tier II
52 NC_000068.7 2 66724741 - Scn7a NNNTTCCTGATTCTAAGAAG NGG 2 0.1648 Tier II
53 NC_000072.6 6 27440671 + Grm8 NNNTTCCTGATTCTAAGAAG NGG 2 0.1648 Tier II
54 NC_000082.6 16 97403513 - Bace2 NNNTTCCAGATGCTCAGATG NGG 2 0.1469 Tier II
55 NC_000082.6 16 60014600 + Epha6 NNNTTGCAGATGCCAAGAAG NGG 2 0.1429 Tier II
56 NC_000081.6 15 84393704 + Shisal1 NNNTTCCAGATCCTAACAAG NGG 2 0.1246 Tier II
57 NC_000082.6 16 5961849 + Rbfox1 NNNATCCAGATGCTAAGAAG NAG 2 0.1235 Tier II
58 NC_000071.6 5 150270209 + Fry NNNCTCCAGATGCTAAGCAG NGG 2 0.1232 Tier II
59 NC_000069.6 3 135380228 + Slc9b1 NNNTTCCAGATGCCAAGAAC NGG 2 0.1224 Tier II
60 NC_000075.6 9 23405041 + Bmper NNNTTCCAGATGCTCAGAAC NGG 2 0.1169 Tier II
61 NC_000082.6 16 7073041 + Rbfox1 NNNTTCCAGATGCTAACAAC NGG 2 0.1008 Tier II
62 NC_000074.6 8 84744622 - Nfix NNNTTCCAGATGCCAATAAG NGG 2 0.0714 Tier II
63 NC_000080.6 14 10314003 + Fhit NNNTTCCAGATGCTTAGAAG NAG 2 0.0519 Tier II
64 NC_000084.6 18 72069970 - Dcc NNNTTCCAGATGCTAAGTAG NTG 2 0.0195 Tier II
65 NC_000085.6 19 40384061 + Sorbs1 NNNTTCCAGATGTTAAGAAG NGC 2 0.0156 Tier II
66 NC_000075.6 9 48491629 + Rbm7 NNNGTCCAGATGCTAAGAAG NGC 2 0.0139 Tier II
67 NC_000069.6 3 18142636 + Cyp7b1 NNNTTGCAGATGCTAAGAAG NGC 2 0.0111 Tier II
68 NC_000082.6 16 26981143 - Gmnc NNNTTGCAGATGCTAAGAAG NGC 2 0.0111 Tier II
69 NC_000084.6 18 22438171 - Asxl3 NNNTTGCAGATGCTAAGAAG NGC 2 0.0111 Tier II
70 NC_000079.6 13 46701414 - Nup153 NNNTTCCACATGCTAAGAAG NGT 2 0.0087 Tier II
71 NC_000084.6 18 38315041 - Rnf14 NNNTTCCAGATGCTAGGAAG NTG 2 0.0075 Tier II
72 NC_000075.6 9 22764974 + Bbs9 NNNTTCCAGATGCCAAGAAG NGT 2 0.0046 Tier II
73 NC_000082.6 16 43237953 - Zbtb20 NNNTTCCAGATGCTAAGAAG NTA 2 0.0 Tier II
74 NC_000067.6 1 152228568 + Gm36920 NNNTTCTAGATGCAAAGAAG NGG 2 0.503 Tier III
75 NC_000079.6 13 103580402 + Gm46398 NNNTACCAGGTGCTAAGAAG NGG 2 0.2778 Tier III
76 NC_000074.6 8 64418557 + Gm35521 NNNTTCCCAATGCTAAGAAG NGG 2 0.2755 Tier III
77 NC_000086.7 X 100681929 + Gm52438 NNNTTCCCAATGCTAAGAAG NGG 2 0.2755 Tier III
78 NC_000074.6 8 50664183 + 1700019L22Rik NNNTGCCTGATGCTAAGAAG NGG 2 0.2743 Tier III
79 NC_000068.7 2 42917814 - Gm39803 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier III
80 NC_000072.6 6 40917618 - Tcrb NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier III
81 NC_000072.6 6 70583982 - Igk NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier III
82 NC_000072.6 6 80141025 - Gm32091 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier III
83 NC_000073.6 7 61073927 + A230006K03Rik NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier III
84 NC_000076.6 10 50102806 - Gm40642 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier III
85 NC_000078.6 12 58000796 + Gm46329 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier III
86 NC_000078.6 12 68658204 + Gm31307 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier III
87 NC_000078.6 12 114629334 - Igh NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier III
88 NC_000084.6 18 25925594 + Gm33228 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier III
89 NC_000084.6 18 25927840 + Gm33228 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier III
90 NC_000086.7 X 3468882 + Gm46694 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier III
91 NC_000086.7 X 3530197 - Gm53053 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier III
92 NC_000086.7 X 4019637 - Gm14355 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier III
93 NC_000086.7 X 4352600 - Gm14362 NNNTTCCAAATTCTAAGAAG NGG 2 0.2473 Tier III
94 NC_000072.6 6 68379019 + Igk NNNTTCCACATTCTAAGAAG NGG 2 0.2071 Tier III
95 NC_000067.6 1 7369430 - Gm26901 NNNTTCCTGATTCTAAGAAG NGG 2 0.1648 Tier III
96 NC_000077.6 11 64668851 + Gm30305 NNNTTCCTGATTCTAAGAAG NGG 2 0.1648 Tier III
97 NC_000080.6 14 8061072 + Gm52100 NNNTTGCAGATGCCAAGAAG NGG 2 0.1429 Tier III
98 NC_000084.6 18 23917742 - Gm38559 NNNTTCCAGATGCTAAGATG NGA 2 0.0374 Tier III
99 NC_000074.6 8 93671976 + Gm26843 NNNTTCCAGATGCTAAGAAA NGC 2 0.0208 Tier III
100 NC_000084.6 18 47939393 + G630055G22Rik NNNTTCCAGATGCTAAGAAT NGC 2 0.0156 Tier III
101 NC_000083.6 17 64516147 - AU016765 NNNTTCCAGATGCTTAGAAG NGA 2 0.0139 Tier III
102 NC_000068.7 2 132152478 - Gm14051 NNNTTCTAGATGCTAAGAAG NGT 2 0.0131 Tier III
103 NC_000072.6 6 68489590 - Igk NNNTTGCAGATGCTAAGAAG NGC 2 0.0111 Tier III
104 NC_000072.6 6 70496233 - Igk NNNTTGCAGATGCTAAGAAG NGC 2 0.0111 Tier III
105 NC_000077.6 11 44438370 + Gm42073 NNNTTGCAGATGCTAAGAAG NGC 2 0.0111 Tier III
106 NC_000068.7 2 147263038 - Nkx2-2os NNNTTCCAGATGCTGAGAAG NGT 2 0.0105 Tier III
107 NC_000071.6 5 9769872 + Gm35139 NNNTTACAGATGCTATGAAG NGG 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)