Construct: sgRNA BRDN0001146473
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTCAGGAACCGACTCCGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ITPKB (3707)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76873
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
226736808 |
- |
ITPKB |
NNNCAGGAACCGACTCCGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
88861745 |
- |
TRAPPC2L |
NNNCTGGAACCAACTCCGGG |
NGG |
2 |
0.3394 |
Tier II |
| 3 |
NC_000019.10 |
19 |
6214124 |
- |
MLLT1 |
NNNCAGGCACCGACTCCGTG |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000010.11 |
10 |
132646889 |
- |
INPP5A |
NNNCAGGTCCCGACTCCGGG |
NGG |
2 |
0.2449 |
Tier II |
| 5 |
NC_000018.10 |
18 |
32281134 |
+ |
GAREM1 |
NNNCAGGAACTGACTCAGGG |
NGG |
2 |
0.1436 |
Tier II |
| 6 |
NC_000019.10 |
19 |
590589 |
+ |
HCN2 |
NNNCAGGTACCGCCTCCGGG |
NGG |
2 |
0.0902 |
Tier II |
| 7 |
NC_000012.12 |
12 |
43864186 |
- |
TMEM117 |
NNNCAGGAACCCACTGCGGG |
NGG |
2 |
0.0814 |
Tier II |
| 8 |
NC_000019.10 |
19 |
590589 |
+ |
LOC100420586 |
NNNCAGGTACCGCCTCCGGG |
NGG |
2 |
0.0902 |
Tier III |
| 9 |
NC_000020.11 |
20 |
1711599 |
+ |
SIRPB3P |
NNNCAGGAACCGACTTGGGG |
NGG |
2 |
0.0181 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
136443126 |
- |
Htr1d |
NNNCAGGATTCGACTCCGGG |
NGG |
2 |
0.5647 |
Tier I |
| 2 |
NC_000081.6 |
15 |
101453519 |
- |
Krt88 |
NNNCAGGAACTGACTCCGGG |
NGC |
2 |
0.0068 |
Tier I |
| 3 |
NC_000074.6 |
8 |
122550051 |
- |
Piezo1 |
NNNCAGGAACAGGCTCCGGG |
NGG |
2 |
0.4891 |
Tier II |
| 4 |
NC_000085.6 |
19 |
25137074 |
- |
Dock8 |
NNNCAGGAACCAACTCAGGG |
NGG |
2 |
0.4356 |
Tier II |
| 5 |
NC_000068.7 |
2 |
162658124 |
- |
Ptprt |
NNNCAGGGACCGACTCTGGG |
NGG |
2 |
0.3422 |
Tier II |
| 6 |
NC_000069.6 |
3 |
152754235 |
- |
Pigk |
NNNCAGGAACCGACTCCGGG |
NAG |
1 |
0.2593 |
Tier II |
| 7 |
NC_000075.6 |
9 |
102207093 |
+ |
Ephb1 |
NNNCAGGAACGGACTCCTGG |
NGG |
2 |
0.1667 |
Tier II |
| 8 |
NC_000069.6 |
3 |
152754235 |
- |
Gm40181 |
NNNCAGGAACCGACTCCGGG |
NAG |
1 |
0.2593 |
Tier III |
Other clones with same target sequence:
(none)
