Construct: sgRNA BRDN0001146486

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AACTTACTGTTTGCATGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGK1 (6446)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77459

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 134174521 + SGK1 NNNTTACTGTTTGCATGCAT NGG 0 1.0 Tier I
2 NC_000011.10 11 34090669 - CAPRIN1 NNNTTACCGTTTGCATGGAT NGG 2 0.1333 Tier I
3 NC_000002.12 2 26977355 + MAPRE3 NNNTTACTGTTTACATGTAT NGG 2 0.5934 Tier II
4 NC_000002.12 2 88102005 + SMYD1 NNNTTTCTGTATGCATGCAT NGG 2 0.5357 Tier II
5 NC_000015.10 15 98767177 + IGF1R NNNTTACTTTTTACATGCAT NGG 2 0.4923 Tier II
6 NC_000010.11 10 87525343 + MINPP1 NNNTTTCTGTCTGCATGCAT NGG 2 0.4762 Tier II
7 NC_000006.12 6 46427311 + RCAN2 NNNTTACTGGTTACATGCAT NGG 2 0.4615 Tier II
8 NC_000013.11 13 52706260 + CNMD NNNTTACTGTTTGCAGACAT NGG 2 0.3231 Tier II
9 NC_000015.10 15 86114795 + AGBL1 NNNGAACTGTTTGCATGCAT NGG 2 0.3125 Tier II
10 NC_000011.10 11 115391834 - CADM1 NNNTTCCTGTCTGCATGCAT NGG 2 0.303 Tier II
11 NC_000022.11 22 33632667 - LARGE1 NNNTTACTGCTTGCATGCTT NGG 2 0.2872 Tier II
12 NC_000005.10 5 39018135 + RICTOR NNNTTAGTGTTTGCATGAAT NGG 2 0.2534 Tier II
13 NC_000020.11 20 54529778 + DOK5 NNNTTTCTGTTTTCATGCAT NGG 2 0.2143 Tier II
14 NC_000011.10 11 132148180 - NTM NNNTTGCTGTTTGCATGCAT NAG 2 0.1852 Tier II
15 NC_000016.10 16 23063787 + USP31 NNNTTATTGTTTGCTTGCAT NGG 2 0.1625 Tier II
16 NC_000017.11 17 19151475 - GRAPL NNNTTACTGTTTCCATGCGT NGG 2 0.1579 Tier II
17 NC_000007.14 7 84193801 - SEMA3A NNNTTACTGTCTGCTTGCAT NGG 2 0.1333 Tier II
18 NC_000006.12 6 95601349 - MANEA NNNTTACTCTTTGCATGCAG NGG 2 0.095 Tier II
19 NC_000020.11 20 1241488 - RAD21L1 NNNTTACTGTTTGCATGCCT NAG 2 0.0536 Tier II
20 NC_000002.12 2 182578714 - PDE1A NNNTTACTGTTTGCATGGAG NGG 2 0.0235 Tier II
21 NC_000003.12 3 19203095 - KCNH8 NNNTTCCTGTTTGCATGCAT NGT 2 0.0073 Tier II
22 NC_000004.12 4 41917649 - LOC105374426 NNNATATTGTTTGCATGCAT NGG 2 0.3869 Tier III
23 NC_000015.10 15 86114795 + LINC01584 NNNGAACTGTTTGCATGCAT NGG 2 0.3125 Tier III
24 NC_000006.12 6 152851252 + LINC02840 NNNTTTCTGTGTGCATGCAT NGG 2 0.2857 Tier III
25 NC_000003.12 3 181627805 - SOX2-OT NNNTTACTATTTGCAGGCAT NGG 2 0.2225 Tier III
26 NC_000021.9 21 32849936 - LOC124905012 NNNTTACTGCTTGCATGCAT NAG 2 0.1383 Tier III
27 NC_000007.14 7 112683708 + LOC101928012 NNNTTACTGTTTGCCTGCAT NTG 2 0.0106 Tier III
28 NC_000009.12 9 90394698 + LINC01508 NNNTTACTGTTTGCATGCAT NAT 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 21995032 - Sgk1 NNNTTACTGTTTGCATGCAT NGG 0 1.0 Tier I
2 NC_000080.6 14 30102367 - Cacna1d NNNTTCATGTTTGCATGCAT NGG 2 0.3409 Tier I
3 NC_000073.6 7 24207445 + Zfp111 NNNTTACTGTTTGCATACAT NGG 1 0.9333 Tier II
4 NC_000073.6 7 139648332 + Cfap46 NNNTTGCTGTTTGTATGCAT NGG 2 0.5238 Tier II
5 NC_000081.6 15 26334664 - Marchf11 NNNTGACTGTCTGCATGCAT NGG 2 0.4267 Tier II
6 NC_000080.6 14 20154962 + Kcnk5 NNNTTACTCTCTGCATGCAT NGG 2 0.359 Tier II
7 NC_000084.6 18 37331491 - Gm37013 NNNTTACTGTCTGCATGCTT NGG 2 0.359 Tier II
8 NC_000084.6 18 37331491 - Gm38666 NNNTTACTGTCTGCATGCTT NGG 2 0.359 Tier II
9 NC_000084.6 18 37331491 - Gm38667 NNNTTACTGTCTGCATGCTT NGG 2 0.359 Tier II
10 NC_000068.7 2 125573264 - Cep152 NNNTTATTGTTTCCATGCAT NGG 2 0.3421 Tier II
11 NC_000079.6 13 93051595 - Cmya5 NNNTTTCTGTTTGCAGGCAT NGG 2 0.2473 Tier II
12 NC_000069.6 3 83330526 + Dchs2 NNNTTAGTGTGTGCATGCAT NGG 2 0.1882 Tier II
13 NC_000072.6 6 98314862 + Gm765 NNNTTACTGTTTGCATGAAT NAG 2 0.1396 Tier II
14 NC_000079.6 13 42062482 + Hivep1 NNNTTACTGTTTGCATGCAT NTG 1 0.039 Tier II
15 NC_000077.6 11 94312765 + Luc7l3 NNNTTACTGTATGCATGCAT NTG 2 0.0292 Tier II
16 NC_000067.6 1 107516490 - Serpinb2 NNNTTACTATTTGCATGCAT NTG 2 0.025 Tier II
17 NC_000083.6 17 30257869 - Btbd9 NNNTTAATGTTTGCATGCAT NGC 2 0.0167 Tier II
18 NC_000067.6 1 82736313 - Mff NNNTTACTGTTTGCATGCCT NGA 2 0.0144 Tier II
19 NC_000078.6 12 79632602 + Rad51b NNNTTACTGTTTTCATGCAT NTG 2 0.0117 Tier II
20 NC_000071.6 5 32647275 - Yes1 NNNTTACTATTTGCATGCAT NGT 2 0.0104 Tier II
21 NC_000074.6 8 34162315 + Saraf NNNTTACTGTTTGCATGCAT NTC 2 0.0 Tier II
22 NC_000084.6 18 80535540 + Gm52341 NNNTTACATTTTGCATGCAT NGG 2 0.4267 Tier III
23 NC_000079.6 13 33720265 + Gm11392 NNNTTTCTGTTTCCATGCAT NGG 2 0.3008 Tier III
24 NC_000070.6 4 95010731 + Gm12694 NNNTTACTGTTTGCACGCAC NGG 2 0.0606 Tier III
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Other clones with same target sequence:

(none)