Construct: sgRNA BRDN0001146495
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGAGTATTACAATGTGCCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCG (5582)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76900
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
53893032 |
+ |
PRKCG |
NNNGTATTACAATGTGCCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
96990976 |
+ |
LCOR |
NNNGTATTACAATATGCCAG |
NGG |
2 |
0.5357 |
Tier II |
| 3 |
NC_000001.11 |
1 |
232001482 |
- |
DISC1 |
NNNGTATTACAATATGCTGG |
NGG |
2 |
0.4821 |
Tier II |
| 4 |
NC_000014.9 |
14 |
59535121 |
+ |
CCDC175 |
NNNGGATTACAATGTGCCTG |
NGG |
2 |
0.4267 |
Tier II |
| 5 |
NC_000003.12 |
3 |
69051057 |
- |
TMF1 |
NNNGGATTAGAATGTGCCGG |
NGG |
2 |
0.2489 |
Tier II |
| 6 |
NC_000006.12 |
6 |
87515745 |
+ |
RARS2 |
NNNGTATAACAATGTGCCGG |
NGA |
2 |
0.0556 |
Tier II |
| 7 |
NC_000001.11 |
1 |
232001482 |
- |
TSNAX-DISC1 |
NNNGTATTACAATATGCTGG |
NGG |
2 |
0.4821 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
3314177 |
+ |
Prkcg |
NNNATATTACAATGTACCGG |
NGG |
2 |
0.9 |
Tier I |
| 2 |
NC_000083.6 |
17 |
24503856 |
+ |
Caskin1 |
NNNGTATTACAAGGTGCTGG |
NGG |
2 |
0.1677 |
Tier I |
| 3 |
NC_000086.7 |
X |
157941677 |
+ |
Cnksr2 |
NNNTTATTACAATGTGCCAG |
NGG |
2 |
0.2597 |
Tier II |
| 4 |
NC_000083.6 |
17 |
26228150 |
- |
Fam234a |
NNNTTATTACAATGTGCCGC |
NGG |
2 |
0.1558 |
Tier II |
| 5 |
NC_000084.6 |
18 |
37661466 |
- |
Gm37013 |
NNNGTATTACACTGTGCCGG |
NGA |
2 |
0.0183 |
Tier II |
| 6 |
NC_000084.6 |
18 |
37661466 |
- |
Gm38666 |
NNNGTATTACACTGTGCCGG |
NGA |
2 |
0.0183 |
Tier II |
| 7 |
NC_000084.6 |
18 |
37661466 |
- |
Gm38667 |
NNNGTATTACACTGTGCCGG |
NGA |
2 |
0.0183 |
Tier II |
Other clones with same target sequence:
(none)
