Construct: sgRNA BRDN0001146561
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGAAGACTTCCCTGACTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BMX (660)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75654
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
15522562 |
+ |
BMX |
NNNAAGACTTCCCTGACTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
41127729 |
- |
EP300 |
NNNAAGACTTGCCTGACTGG |
NAG |
2 |
0.0648 |
Tier I |
| 3 |
NC_000023.11 |
X |
15522562 |
+ |
ACE2 |
NNNAAGACTTCCCTGACTGG |
NGG |
0 |
1.0 |
Tier II |
| 4 |
NC_000001.11 |
1 |
205356538 |
+ |
KLHDC8A |
NNNAAAACTTACCTGACTGG |
NGG |
2 |
0.75 |
Tier II |
| 5 |
NC_000010.11 |
10 |
104060596 |
- |
COL17A1 |
NNNAGGACTTACCTGACTGG |
NGG |
2 |
0.54 |
Tier II |
| 6 |
NC_000012.12 |
12 |
64559760 |
- |
RASSF3 |
NNNAAGACTTCCTTGACTAG |
NGG |
2 |
0.5 |
Tier II |
| 7 |
NC_000001.11 |
1 |
183759661 |
- |
RGL1 |
NNNGAGACTTCCCTGACTAG |
NGG |
2 |
0.4464 |
Tier II |
| 8 |
NC_000011.10 |
11 |
111428461 |
- |
BTG4 |
NNNAAGAGGTCCCTGACTGG |
NGG |
2 |
0.398 |
Tier II |
| 9 |
NC_000002.12 |
2 |
237875223 |
+ |
RAMP1 |
NNNAAGGCTGCCCTGACTGG |
NGG |
2 |
0.3529 |
Tier II |
| 10 |
NC_000003.12 |
3 |
125579170 |
+ |
OSBPL11 |
NNNCAAACTTCCCTGACTGG |
NGG |
2 |
0.3529 |
Tier II |
| 11 |
NC_000008.11 |
8 |
32196395 |
- |
NRG1 |
NNNAAGCCTTCCCTGACTAG |
NGG |
2 |
0.3125 |
Tier II |
| 12 |
NC_000017.11 |
17 |
79093247 |
- |
RBFOX3 |
NNNGAGCCTTCCCTGACTGG |
NGG |
2 |
0.2734 |
Tier II |
| 13 |
NC_000001.11 |
1 |
183953060 |
- |
COLGALT2 |
NNNAAGTCTTCCCAGACTGG |
NGG |
2 |
0.2708 |
Tier II |
| 14 |
NC_000003.12 |
3 |
36456096 |
- |
STAC |
NNNCATACTTCCCTGACTGG |
NGG |
2 |
0.2353 |
Tier II |
| 15 |
NC_000017.11 |
17 |
1969577 |
- |
RTN4RL1 |
NNNAAGCCTGCCCTGACTGG |
NGG |
2 |
0.2188 |
Tier II |
| 16 |
NC_000020.11 |
20 |
23398067 |
+ |
NAPB |
NNNAAGCCTGCCCTGACTGG |
NGG |
2 |
0.2188 |
Tier II |
| 17 |
NC_000015.10 |
15 |
27327457 |
- |
GABRG3 |
NNNAAGACTTCCCTGACTCC |
NGG |
2 |
0.1921 |
Tier II |
| 18 |
NC_000023.11 |
X |
134622087 |
- |
PLAC1 |
NNNATGCCTTCCCTGACTGG |
NGG |
2 |
0.1591 |
Tier II |
| 19 |
NC_000016.10 |
16 |
70691105 |
+ |
VAC14 |
NNNAAGACTTCCCTGACTGA |
NCG |
2 |
0.1004 |
Tier II |
| 20 |
NC_000003.12 |
3 |
140351112 |
+ |
CLSTN2 |
NNNAAGACTTCCCAGAGTGG |
NGG |
2 |
0.0364 |
Tier II |
| 21 |
NC_000012.12 |
12 |
56140660 |
- |
ESYT1 |
NNNAAGGCTTCCCTGACTGG |
NTG |
2 |
0.0275 |
Tier II |
| 22 |
NC_000011.10 |
11 |
19762909 |
- |
NAV2 |
NNNAAGACTTCCCTGGCTGG |
NCG |
2 |
0.0206 |
Tier II |
| 23 |
NC_000017.11 |
17 |
35183271 |
- |
UNC45B |
NNNAAGCCTTCCCTGACTGG |
NTG |
2 |
0.017 |
Tier II |
| 24 |
NC_000007.14 |
7 |
33968412 |
- |
BMPER |
NNNAAGACTTCCCTGACAGG |
NGC |
2 |
0.0148 |
Tier II |
| 25 |
NC_000022.11 |
22 |
32621176 |
- |
SYN3 |
NNNGAGACTTCCCTGACTGG |
NGC |
2 |
0.0139 |
Tier II |
| 26 |
NC_000019.10 |
19 |
10769390 |
- |
DNM2 |
NNNAAGACTTCCCTGAGTGG |
NGC |
2 |
0.0013 |
Tier II |
| 27 |
NC_000022.11 |
22 |
32645852 |
+ |
SYN3 |
NNNAAGAATTCCCTGTCTGG |
NGG |
2 |
0.0 |
Tier II |
| 28 |
NC_000008.11 |
8 |
73183877 |
- |
LOC107986891 |
NNNAAGGCTTCACTGACTGG |
NGG |
2 |
0.5042 |
Tier III |
| 29 |
NC_000002.12 |
2 |
238922830 |
+ |
LINC01940 |
NNNCAGACTTCCCTGGCTGG |
NGG |
2 |
0.0679 |
Tier III |
| 30 |
NC_000004.12 |
4 |
54857737 |
- |
LINC02358 |
NNNAAGACTTCCCTGACTGT |
NGA |
2 |
0.0486 |
Tier III |
| 31 |
NC_000009.12 |
9 |
89986995 |
+ |
LOC101927847 |
NNNAACACTTCCCTGCCTGG |
NGG |
2 |
0.0 |
Tier III |
| 32 |
NC_000022.11 |
22 |
32645852 |
+ |
LOC105373002 |
NNNAAGAATTCCCTGTCTGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
164234928 |
- |
Bmx |
NNNAAGACTGCCCTGACTGG |
NGG |
1 |
0.5 |
Tier I |
| 2 |
NC_000067.6 |
1 |
86306408 |
+ |
B3gnt7 |
NNNAGAACTTCCCTGACTGG |
NGG |
2 |
0.72 |
Tier II |
| 3 |
NC_000070.6 |
4 |
106504977 |
- |
Tmem61 |
NNNAGGGCTTCCCTGACTGG |
NGG |
2 |
0.5082 |
Tier II |
| 4 |
NC_000068.7 |
2 |
24383399 |
- |
Psd4 |
NNNAGGACTTCCCTGACTGT |
NGG |
2 |
0.504 |
Tier II |
| 5 |
NC_000080.6 |
14 |
48665425 |
+ |
Otx2 |
NNNAAAACTTCGCTGACTGG |
NGG |
2 |
0.4444 |
Tier II |
| 6 |
NC_000080.6 |
14 |
78293212 |
- |
Tnfsf11 |
NNNAACTCTTCCCTGACTGG |
NGG |
2 |
0.2983 |
Tier II |
| 7 |
NC_000070.6 |
4 |
107559904 |
+ |
Glis1 |
NNNAATACTTCGCTGACTGG |
NGG |
2 |
0.2963 |
Tier II |
| 8 |
NC_000072.6 |
6 |
43444131 |
- |
Tpk1 |
NNNCAGACTTCTCTGACTGG |
NGG |
2 |
0.19 |
Tier II |
| 9 |
NC_000079.6 |
13 |
57638564 |
- |
Spock1 |
NNNAAGACTTCCCTGGCTGA |
NGG |
2 |
0.1803 |
Tier II |
| 10 |
NC_000068.7 |
2 |
103726914 |
- |
Nat10 |
NNNCAGACTGCCCTGACTGG |
NGG |
2 |
0.1765 |
Tier II |
| 11 |
NC_000080.6 |
14 |
74701904 |
- |
Htr2a |
NNNAAGACTTGCCTGACAGG |
NGG |
2 |
0.1667 |
Tier II |
| 12 |
NC_000075.6 |
9 |
58738380 |
- |
Rec114 |
NNNAAGACTTCTCTGACTGG |
NAG |
2 |
0.1396 |
Tier II |
| 13 |
NC_000073.6 |
7 |
39500181 |
- |
Gm28455 |
NNNAAGACTTCCCTGACTGG |
NGC |
1 |
0.0222 |
Tier II |
| 14 |
NC_000073.6 |
7 |
19945486 |
+ |
Igsf23 |
NNNATGACTTCCCTGACTGG |
NTG |
2 |
0.0142 |
Tier II |
| 15 |
NC_000074.6 |
8 |
118389946 |
- |
Cdh13 |
NNNAAGACATCCCTGTCTGG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000067.6 |
1 |
146793023 |
- |
Brinp3 |
NNNAAGACTTCCCTGACTGG |
NCT |
2 |
0.0 |
Tier II |
| 17 |
NC_000067.6 |
1 |
183359716 |
+ |
Mia3 |
NNNAAGACTTCCCTGCCTTG |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000076.6 |
10 |
3264182 |
+ |
H60c |
NNNAAGACTTCCCTGCTTGG |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000075.6 |
9 |
53314428 |
+ |
Exph5 |
NNNAAGACTTGCCTGTCTGG |
NGG |
2 |
0.0 |
Tier II |
| 20 |
NC_000072.6 |
6 |
43444131 |
- |
9430018G01Rik |
NNNCAGACTTCTCTGACTGG |
NGG |
2 |
0.19 |
Tier III |
| 21 |
NC_000067.6 |
1 |
73368387 |
- |
Gm31344 |
NNNAAGATTTCCCTGACTGG |
NTG |
2 |
0.0341 |
Tier III |
| 22 |
NC_000083.6 |
17 |
88255182 |
+ |
Gm31615 |
NNNAAGACTTCCCTGACTGG |
NTT |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
