Construct: sgRNA BRDN0001146686
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGACCATACCAGTGCATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSNK1A1L (122011)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77382
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
37105031 |
+ |
CSNK1A1L |
NNNACCATACCAGTGCATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
130398438 |
+ |
TMEM200A |
NNNAACATACCAGTGCATGT |
NGG |
1 |
0.5714 |
Tier II |
| 3 |
NC_000003.12 |
3 |
125771300 |
- |
LOC112267908 |
NNNACCACACCAGTGCATGA |
NGG |
2 |
0.5625 |
Tier II |
| 4 |
NC_000007.14 |
7 |
97924763 |
+ |
ASNS |
NNNACCACACCAGTGCATGA |
NGG |
2 |
0.5625 |
Tier II |
| 5 |
NC_000002.12 |
2 |
132642529 |
+ |
GPR39 |
NNNACCAGGCCAGTGCATGT |
NGG |
2 |
0.4889 |
Tier II |
| 6 |
NC_000015.10 |
15 |
81207838 |
+ |
IL16 |
NNNAGCATACAAGTGCATGT |
NGG |
2 |
0.45 |
Tier II |
| 7 |
NC_000023.11 |
X |
48061006 |
+ |
ZNF630 |
NNNACCATAGCAGTACATGT |
NGG |
2 |
0.366 |
Tier II |
| 8 |
NC_000008.11 |
8 |
68389444 |
+ |
C8orf34 |
NNNATCATCCCAGTGCATGT |
NGG |
2 |
0.3636 |
Tier II |
| 9 |
NC_000009.12 |
9 |
91856814 |
- |
ROR2 |
NNNAGCATCCCAGTGCATGT |
NGG |
2 |
0.3429 |
Tier II |
| 10 |
NC_000005.10 |
5 |
78959617 |
+ |
ARSB |
NNNACTATACCAGTCCATGT |
NGG |
2 |
0.2532 |
Tier II |
| 11 |
NC_000018.10 |
18 |
35031823 |
+ |
MAPRE2 |
NNNAGCATACCTGTGCATGT |
NGG |
2 |
0.2 |
Tier II |
| 12 |
NC_000004.12 |
4 |
4103129 |
+ |
LOC100130459 |
NNNACCACACCAGTGCATGA |
NGG |
2 |
0.5625 |
Tier III |
| 13 |
NC_000007.14 |
7 |
97924763 |
+ |
CZ1P-ASNS |
NNNACCACACCAGTGCATGA |
NGG |
2 |
0.5625 |
Tier III |
| 14 |
NC_000008.11 |
8 |
12010665 |
+ |
OR7E160P |
NNNACCACACCAGTGCATGA |
NGG |
2 |
0.5625 |
Tier III |
| 15 |
NC_000012.12 |
12 |
8386114 |
+ |
LINC00937 |
NNNACCACACCAGTGCATGA |
NGG |
2 |
0.5625 |
Tier III |
| 16 |
NC_000023.11 |
X |
48061006 |
+ |
ZNF630-AS1 |
NNNACCATAGCAGTACATGT |
NGG |
2 |
0.366 |
Tier III |
| 17 |
NC_000009.12 |
9 |
91856814 |
- |
LOC124902210 |
NNNAGCATCCCAGTGCATGT |
NGG |
2 |
0.3429 |
Tier III |
| 18 |
NC_000004.12 |
4 |
28286750 |
- |
LOC105374557 |
NNNACCATATCAGTGCATGT |
NTG |
2 |
0.0367 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
83436352 |
+ |
Snapc3 |
NNNACCATACCAGACCATGT |
NGG |
2 |
0.1688 |
Tier I |
| 2 |
NC_000077.6 |
11 |
34534800 |
- |
Dock2 |
NNNACCATACCAGTGCATGC |
NGG |
1 |
0.0909 |
Tier II |
| 3 |
NC_000068.7 |
2 |
153297217 |
+ |
Kif3b |
NNNACCATACCAGATCATGT |
NGG |
2 |
0.0884 |
Tier II |
| 4 |
NC_000068.7 |
2 |
110898814 |
- |
Ano3 |
NNNACCATTCCAGTTCATGT |
NGG |
2 |
0.0857 |
Tier II |
| 5 |
NC_000084.6 |
18 |
37438378 |
+ |
Pcdhb12 |
NNNTCCATACCAGTGCATGC |
NGG |
2 |
0.0579 |
Tier II |
| 6 |
NC_000084.6 |
18 |
37438378 |
+ |
Gm37013 |
NNNTCCATACCAGTGCATGC |
NGG |
2 |
0.0579 |
Tier II |
| 7 |
NC_000084.6 |
18 |
37438378 |
+ |
Gm38666 |
NNNTCCATACCAGTGCATGC |
NGG |
2 |
0.0579 |
Tier II |
| 8 |
NC_000084.6 |
18 |
37438378 |
+ |
Gm38667 |
NNNTCCATACCAGTGCATGC |
NGG |
2 |
0.0579 |
Tier II |
| 9 |
NC_000068.7 |
2 |
96646577 |
+ |
Lrrc4c |
NNNACCATGCCAGTGCATGT |
NGA |
2 |
0.0463 |
Tier II |
| 10 |
NC_000075.6 |
9 |
104875110 |
- |
Cpne4 |
NNNAACATACCAGTGCATGT |
NTG |
2 |
0.0223 |
Tier II |
| 11 |
NC_000086.7 |
X |
19241917 |
- |
Gm41892 |
NNNAACATACCAGTGCACGT |
NGG |
2 |
0.2449 |
Tier III |
| 12 |
NC_000077.6 |
11 |
70629712 |
- |
Gm12315 |
NNNACCATACCAGTGGATTT |
NGG |
2 |
0.1026 |
Tier III |
| 13 |
NC_000081.6 |
15 |
51381853 |
+ |
Gm19303 |
NNNACCATACCAGGGCATGT |
NGC |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
