Construct: sgRNA BRDN0001146936

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTACCCACAGCATTGTAGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GK (2710)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76328

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 30677440 - GK NNNCCCACAGCATTGTAGAG NGG 0 1.0 Tier I
2 NC_000004.12 4 79407877 + GK2 NNNACCACAGCATTGTAGAG NGG 1 0.8421 Tier I
3 NC_000004.12 4 165279322 + GK3 NNNACCACAGCATTGTAGAG NGG 1 0.8421 Tier I
4 NC_000017.11 17 18292735 - TOP3A NNNCCCAGAGCATTCTAGAG NGG 2 0.1753 Tier I
5 NC_000012.12 12 108702827 - CORO1C NNNCCCCCAGCATTGGAGAG NGG 2 0.1514 Tier I
6 NC_000004.12 4 165279322 + KLHL2 NNNACCACAGCATTGTAGAG NGG 1 0.8421 Tier II
7 NC_000011.10 11 41300523 - LRRC4C NNNCCCACATCATTGAAGAG NGG 2 0.7386 Tier II
8 NC_000004.12 4 25715464 - SEL1L3 NNNCCCACAGCATTGTAAAA NGG 2 0.649 Tier II
9 NC_000016.10 16 66520211 + TK2 NNNCCTACAGCATTGCAGAG NGG 2 0.619 Tier II
10 NC_000014.9 14 63471608 - PPP2R5E NNNCTCACAGCATTGTAGAA NGG 2 0.5966 Tier II
11 NC_000008.11 8 132271305 + KCNQ3 NNNCCCACATCATTGTAAAG NGG 2 0.5625 Tier II
12 NC_000002.12 2 216426356 + SMARCAL1 NNNCACATAGCATTGTAGAG NGG 2 0.5 Tier II
13 NC_000009.12 9 91215745 - AUH NNNCCCACTGCAATGTAGAG NGG 2 0.4154 Tier II
14 NC_000010.11 10 71279582 + UNC5B NNNCACACAGCATTGCAGAG NGG 2 0.381 Tier II
15 NC_000001.11 1 211952417 - INTS7 NNNCTCACAGCATTGTAGTG NGG 2 0.3427 Tier II
16 NC_000014.9 14 104927452 + PLD4 NNNGCCACAGCATTGCAGAG NGG 2 0.3333 Tier II
17 NC_000013.11 13 49553673 + RCBTB1 NNNCCCACAGCAATGTAGGG NGG 2 0.2596 Tier II
18 NC_000014.9 14 77282862 - POMT2 NNNCCCACAGCATTGCAGGG NGG 2 0.25 Tier II
19 NC_000003.12 3 9747611 + BRPF1 NNNCTCACAGCATTGTAGGG NGG 2 0.2386 Tier II
20 NC_000003.12 3 43052937 - GASK1A NNNCCCACAGCATTTTAGAG NGG 1 0.1429 Tier II
21 NC_000012.12 12 104824717 - SLC41A2 NNNCCCACAGCATTGTCTAG NGG 2 0.1176 Tier II
22 NC_000012.12 12 93851217 - CRADD NNNCCCACAGCATTGCTGAG NGG 2 0.0889 Tier II
23 NC_000002.12 2 63321656 + WDPCP NNNCCCACAGCATTGTAGAG NGA 1 0.0694 Tier II
24 NC_000001.11 1 53074902 + PODN NNNCCCACAGGAGTGTAGAG NGG 2 0.0652 Tier II
25 NC_000016.10 16 87915362 + CA5A NNNCCCACAGCATTGTAGAA NGA 2 0.0651 Tier II
26 NC_000003.12 3 126452327 - ZXDC NNNCCCACAGCATTGTAGTG NGA 2 0.0374 Tier II
27 NC_000002.12 2 80387909 + CTNNA2 NNNCCCACAGCAATGTAGAG NGT 2 0.0112 Tier II
28 NC_000005.10 5 107507203 + EFNA5 NNNCCCACAGCATTTTAGAG NTG 2 0.0056 Tier II
29 NC_000011.10 11 79236801 + TENM4 NNNCCCACAGGATTGTAGAG NGC 2 0.0056 Tier II
30 NC_000014.9 14 59367717 - DAAM1 NNNCCCACAGCATTCTAGAG NGT 2 0.0044 Tier II
31 NC_000010.11 10 85780719 - GRID1 NNNCCCACAGCATGGCAGAG NGG 2 0.0 Tier II
32 NC_000004.12 4 119394634 + GK6P NNNACCACAGCATTGTAGAG NGG 1 0.8421 Tier III
33 NC_000004.12 4 119394634 + LOC107986193 NNNACCACAGCATTGTAGAG NGG 1 0.8421 Tier III
34 NC_000023.11 X 101780217 + GK4P NNNACCACAGCATTGTAGAG NGG 1 0.8421 Tier III
35 NC_000016.10 16 22421587 + RRN3P3 NNNCCCACAGCATTGTAGAA NGA 2 0.0651 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 52694046 - Gykl1 NNNACCACAGCATTGTAGAG NGG 1 0.8421 Tier I
2 NC_000086.7 X 85754345 + Gk NNNCCCACGGCATTATAGAG NGG 2 0.6275 Tier I
3 NC_000068.7 2 97568997 + Lrrc4c NNNCCCTCAGCATTGTAGAG NGG 1 0.4375 Tier II
4 NC_000069.6 3 37497289 - Spata5 NNNACCCCAGCATTGTAGAG NGG 2 0.3684 Tier II
5 NC_000077.6 11 110244772 - Abca6 NNNCCCCCTGCATTGTAGAG NGG 2 0.2625 Tier II
6 NC_000070.6 4 87882211 + Mllt3 NNNCCAACAGCATTCTAGAG NGG 2 0.2532 Tier II
7 NC_000082.6 16 43046683 + Zbtb20 NNNCCCACAGCAGTGTAGAA NGG 2 0.2446 Tier II
8 NC_000070.6 4 141954944 - Fhad1 NNNCCCACAGCAGTGTAGAT NGG 2 0.1826 Tier II
9 NC_000073.6 7 45185881 - Kash5 NNNCCCACAGCAGTGTATAG NGG 2 0.1739 Tier II
10 NC_000070.6 4 153997805 + Cep104 NNNCCCAGAGCCTTGTAGAG NGG 2 0.1692 Tier II
11 NC_000071.6 5 115367980 - Msi1 NNNCCCACAGCACTTTAGAG NGG 2 0.1128 Tier II
12 NC_000073.6 7 142085335 + Dusp8 NNNCACACAGCATTGTTGAG NGG 2 0.0762 Tier II
13 NC_000069.6 3 86836502 + Dclk2 NNNCCCACAGCACTGTAGAG NGA 2 0.0548 Tier II
14 NC_000081.6 15 37449059 + Ncald NNNCCCACAGCATTGTAGAG NTG 1 0.039 Tier II
15 NC_000080.6 14 16694710 - Rarb NNNCCCACAGCATTGGAGAG NGA 2 0.024 Tier II
16 NC_000085.6 19 29352282 + Plgrkt NNNCCCACAGCATTGAAGAG NGT 2 0.0147 Tier II
17 NC_000077.6 11 62022971 - Specc1 NNNCCCACAGCATGCTAGAG NGG 2 0.0 Tier II
18 NC_000074.6 8 128226571 - A930035F09Rik NNNCCAACAGCAGTGTAGAG NGG 2 0.2422 Tier III
19 NC_000070.6 4 153997805 + Gm31566 NNNCCCAGAGCCTTGTAGAG NGG 2 0.1692 Tier III
20 NC_000078.6 12 58564627 + Gm30687 NNNCCCACAGCTTTCTAGAG NGG 2 0.0909 Tier III
21 NC_000077.6 11 94159305 + Gm35338 NNNCCCACAGGATTGTAGAG NAG 2 0.0648 Tier III
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Other clones with same target sequence:

(none)