Construct: sgRNA BRDN0001147020
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTTGGCTAATGATGTACCT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAST4 (375449)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76884
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
67114177 |
- |
MAST4 |
NNNTGGCTAATGATGTACCT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
60324821 |
+ |
FHIT |
NNNTGGCTGATGATATACCT |
NGG |
2 |
0.6275 |
Tier II |
| 3 |
NC_000011.10 |
11 |
26371217 |
- |
ANO3 |
NNNTGGCTCAAGATGTACCT |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000004.12 |
4 |
134095827 |
- |
PABPC4L |
NNNTGGCCAAGGATGTACCT |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000008.11 |
8 |
10694404 |
+ |
C8orf74 |
NNNAGGTTAATGATGTACCT |
NGG |
2 |
0.3869 |
Tier II |
| 6 |
NC_000011.10 |
11 |
66792377 |
- |
TOP6BL |
NNNTGGCTTATTATGTACCT |
NGG |
2 |
0.2308 |
Tier II |
| 7 |
NC_000001.11 |
1 |
202662543 |
- |
SYT2 |
NNNTGGTTAATGATGTACCT |
NAG |
2 |
0.2106 |
Tier II |
| 8 |
NC_000001.11 |
1 |
201776889 |
- |
NAV1 |
NNNTCGCTAATGATGTGCCT |
NGG |
2 |
0.1387 |
Tier II |
| 9 |
NC_000006.12 |
6 |
56133775 |
- |
COL21A1 |
NNNTGGCTCATGCTGTACCT |
NGG |
2 |
0.1203 |
Tier II |
| 10 |
NC_000006.12 |
6 |
144649442 |
- |
UTRN |
NNNTGGCTAATGATGTGACT |
NGG |
2 |
0.095 |
Tier II |
| 11 |
NC_000023.11 |
X |
111093894 |
- |
PAK3 |
NNNTGGCTAATGATTTAACT |
NGG |
2 |
0.0769 |
Tier II |
| 12 |
NC_000002.12 |
2 |
68812740 |
+ |
ARHGAP25 |
NNNTGGCTACTGATGTCCCT |
NGG |
2 |
0.0588 |
Tier II |
| 13 |
NC_000012.12 |
12 |
111236292 |
- |
CUX2 |
NNNTGGCTACTGATGTTCCT |
NGG |
2 |
0.0444 |
Tier II |
| 14 |
NC_000001.11 |
1 |
71991486 |
+ |
NEGR1 |
NNNTGGCTAATGCTGTACCT |
NGA |
2 |
0.0146 |
Tier II |
| 15 |
NC_000011.10 |
11 |
27075019 |
+ |
BBOX1 |
NNNTGGCTAATGTGGTACCT |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000003.12 |
3 |
60324821 |
+ |
LOC107986015 |
NNNTGGCTGATGATATACCT |
NGG |
2 |
0.6275 |
Tier III |
| 17 |
NC_000001.11 |
1 |
191909071 |
+ |
LINC02770 |
NNNTGGCTAAAGCTGTACCT |
NGG |
2 |
0.1579 |
Tier III |
| 18 |
NC_000001.11 |
1 |
201776889 |
- |
IPO9-AS1 |
NNNTCGCTAATGATGTGCCT |
NGG |
2 |
0.1387 |
Tier III |
| 19 |
NC_000001.11 |
1 |
201776889 |
- |
LOC124904483 |
NNNTCGCTAATGATGTGCCT |
NGG |
2 |
0.1387 |
Tier III |
| 20 |
NC_000009.12 |
9 |
13829205 |
- |
LOC101929507 |
NNNTGGCTACTGCTGTACCT |
NGG |
2 |
0.0702 |
Tier III |
| 21 |
NC_000011.10 |
11 |
27075019 |
+ |
BBOX1-AS1 |
NNNTGGCTAATGTGGTACCT |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
102787360 |
+ |
Mast4 |
NNNTGGCTGATGATATACCT |
NGG |
2 |
0.6275 |
Tier I |
| 2 |
NC_000071.6 |
5 |
22051255 |
- |
Reln |
NNNTGCCTGATGATGTACCT |
NGG |
2 |
0.4545 |
Tier II |
| 3 |
NC_000078.6 |
12 |
69342051 |
- |
Nemf |
NNNTGGCTACTGATATACCT |
NGG |
2 |
0.3137 |
Tier II |
| 4 |
NC_000068.7 |
2 |
70804796 |
+ |
Tlk1 |
NNNTAGCTAATGTTGTACCT |
NGG |
2 |
0.26 |
Tier II |
| 5 |
NC_000074.6 |
8 |
68804186 |
- |
Ints10 |
NNNGGGCTAATGATGGACCT |
NGG |
2 |
0.2163 |
Tier II |
| 6 |
NC_000075.6 |
9 |
59477901 |
+ |
Arih1 |
NNNTGGCTAAAGATGTACCT |
NAG |
2 |
0.1944 |
Tier II |
| 7 |
NC_000071.6 |
5 |
48080588 |
+ |
Slit2 |
NNNTGGCTAATAATTTACCT |
NGG |
2 |
0.1333 |
Tier II |
| 8 |
NC_000073.6 |
7 |
120120003 |
- |
Tmem159 |
NNNTGGCTAATGATGTGTCT |
NGG |
2 |
0.1134 |
Tier II |
| 9 |
NC_000076.6 |
10 |
75740379 |
- |
Cabin1 |
NNNTGGCTGATGATGTAGCT |
NGG |
2 |
0.0889 |
Tier II |
| 10 |
NC_000086.7 |
X |
104138768 |
- |
Nexmif |
NNNTGGCTAATGATGTACCA |
NCG |
2 |
0.0603 |
Tier II |
| 11 |
NC_000081.6 |
15 |
89400825 |
+ |
Syce3 |
NNNTGGCTTATGATGTACCC |
NGG |
2 |
0.0545 |
Tier II |
| 12 |
NC_000086.7 |
X |
100648073 |
- |
Kif4 |
NNNTGCCTAATGATGTACCT |
NGT |
2 |
0.011 |
Tier II |
| 13 |
NC_000076.6 |
10 |
91914764 |
- |
Gm31592 |
NNNTGCCTAATGATGTATCT |
NGG |
2 |
0.4383 |
Tier III |
| 14 |
NC_000075.6 |
9 |
59477901 |
+ |
Gm20199 |
NNNTGGCTAAAGATGTACCT |
NAG |
2 |
0.1944 |
Tier III |
| 15 |
NC_000068.7 |
2 |
128201943 |
- |
Gm14009 |
NNNTTGCTAATGATGTATCT |
NGG |
2 |
0.1929 |
Tier III |
Other clones with same target sequence:
(none)
