Construct: sgRNA BRDN0001147188

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTGGCCTGGAAAATCAGATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKL4 (344387)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76109

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 39190341 - CDKL4 NNNGCCTGGAAAATCAGATG NGG 0 1.0 Tier I
2 NC_000014.9 14 50341068 - CDKL1 NNNGCCAGGAAAATCGGATG NGG 2 0.1683 Tier I
3 NC_000003.12 3 129126694 - ISY1-RAB43 NNNGGCTGGAAAGTCAGATG NGG 2 0.3913 Tier II
4 NC_000015.10 15 29738547 + TJP1 NNNGCCTGAGAAATCAGATG NGG 2 0.3571 Tier II
5 NC_000005.10 5 157554473 - ADAM19 NNNGCCTGCAGAATCAGATG NGG 2 0.35 Tier II
6 NC_000001.11 1 33913304 - CSMD2 NNNGCCTGGAAACTCAGATA NGG 2 0.1974 Tier II
7 NC_000016.10 16 19883041 - GPRC5B NNNGCCAGGAAAATAAGATG NGG 2 0.1944 Tier II
8 NC_000005.10 5 14312123 - TRIO NNNTCCTGTAAAATCAGATG NGG 2 0.1939 Tier II
9 NC_000002.12 2 154413553 - GALNT13 NNNGGCTGGAAAACCAGATG NGG 2 0.1714 Tier II
10 NC_000003.12 3 11307507 - ATG7 NNNGCCTTGAAAATCAGATG NAG 2 0.162 Tier II
11 NC_000003.12 3 179806230 + PEX5L NNNGCCGGGAAAATCAGATG NAG 2 0.1525 Tier II
12 NC_000002.12 2 131473272 - MZT2A NNNGCCGGGAAAATCAGAGG NGG 2 0.1471 Tier II
13 NC_000010.11 10 75547616 - LRMDA NNNGTCTGGAAACTCAGATG NGG 2 0.134 Tier II
14 NC_000020.11 20 15486303 - MACROD2 NNNGCCTGGAAAACCAGGTG NGG 2 0.1143 Tier II
15 NC_000008.11 8 142468873 - ADGRB1 NNNGCCTGGACAATCAGAAG NGG 2 0.1143 Tier II
16 NC_000012.12 12 43446058 + ADAMTS20 NNNGCCTGGAAAATCAAATG NGA 2 0.0648 Tier II
17 NC_000011.10 11 79100412 - TENM4 NNNGCCTGGAAAATCAGAGG NAG 2 0.0648 Tier II
18 NC_000002.12 2 43504731 - THADA NNNGCCTGGCAAATCAGCTG NGG 2 0.0635 Tier II
19 NC_000015.10 15 79317536 + TMED3 NNNGCCTGGAAAATCAAATG NGC 2 0.0207 Tier II
20 NC_000009.12 9 120535318 - CDK5RAP2 NNNGCTTGGAAAATCAGATG NGC 2 0.0206 Tier II
21 NC_000004.12 4 20986749 - KCNIP4 NNNTCCTGGAAAATGAGATG NGG 2 0.0182 Tier II
22 NC_000013.11 13 79526836 - NDFIP2 NNNGGCTGGAAAATCAGATG NGC 2 0.0133 Tier II
23 NC_000002.12 2 174851647 + CHN1 NNNGCCTGGAAAATGGGATG NGG 2 0.0096 Tier II
24 NC_000012.12 12 100868617 - ANO4 NNNGCCTGGAAAATGGGATG NGG 2 0.0096 Tier II
25 NC_000020.11 20 61347880 + CDH4 NNNGCCTGGAATATCAGATG NGT 2 0.0054 Tier II
26 NC_000011.10 11 117692223 - DSCAML1 NNNGCCTGGAAAAGCAGAGG NGG 2 0.0 Tier II
27 NC_000001.11 1 110061937 - ALX3 NNNGCCTGGAAAAGGAGATG NGG 2 0.0 Tier II
28 NC_000014.9 14 50750152 - NIN NNNGCCTGGAACATCTGATG NGG 2 0.0 Tier II
29 NC_000023.11 X 110380069 - AMMECR1 NNNGCCTGGGAAATCTGATG NGG 2 0.0 Tier II
30 NC_000023.11 X 110380069 - RTL9 NNNGCCTGGGAAATCTGATG NGG 2 0.0 Tier II
31 NC_000005.10 5 132424484 + CARINH NNNGCATGGAAAAACAGATG NGG 2 0.5748 Tier III
32 NC_000005.10 5 132424484 + LINC02863 NNNGCATGGAAAAACAGATG NGG 2 0.5748 Tier III
33 NC_000008.11 8 124938482 + LOC105375742 NNNTCCTGGAAAATCAGATG NGG 1 0.3636 Tier III
34 NC_000024.10 Y 18603691 + OFD1P5Y NNNGCCTGGAGAATCAGAGG NGG 2 0.1625 Tier III
35 NC_000024.10 Y 18716712 - OFD1P6Y NNNGCCTGGAGAATCAGAGG NGG 2 0.1625 Tier III
36 NC_000009.12 9 86524464 - LOC102724080 NNNGCCTGGAAAAAAAGATG NGG 2 0.1376 Tier III
37 NC_000008.11 8 142468873 - LOC124902075 NNNGCCTGGACAATCAGAAG NGG 2 0.1143 Tier III
38 NC_000018.10 18 34931899 - LOC105372061 NNNGCCTGGATAATCAGATG NGA 2 0.0214 Tier III
39 NC_000006.12 6 84439556 + LINC01611 NNNGCCTGGAAAATCTGATG NAG 2 0.0 Tier III
40 NC_000006.12 6 84439556 + LOC107986620 NNNGCCTGGAAAATCTGATG NAG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 70125787 + Fam81a NNNGCCAGGAAAATCAGATG NGG 1 0.875 Tier II
2 NC_000074.6 8 108850263 - Zfhx3 NNNCCCTAGAAAATCAGATG NGG 2 0.5294 Tier II
3 NC_000080.6 14 34863997 + Grid1 NNNGCCTGGAAGAACAGATG NGG 2 0.4471 Tier II
4 NC_000072.6 6 34133735 - Lrguk NNNGCCAGGAAAATCAGTTG NGG 2 0.4375 Tier II
5 NC_000079.6 13 55668585 - Ddx46 NNNGCCTGGGAAGTCAGATG NGG 2 0.3623 Tier II
6 NC_000076.6 10 44312000 - Atg5 NNNGAGTGGAAAATCAGATG NGG 2 0.2857 Tier II
7 NC_000072.6 6 99640219 + Eif4e3 NNNACCTGGAAAACCAGATG NGG 2 0.2571 Tier II
8 NC_000079.6 13 57866451 + Spock1 NNNGCCAGGAAAATCAGAAG NGG 2 0.25 Tier II
9 NC_000084.6 18 77344720 - Loxhd1 NNNGCATGGAAAATCATATG NGG 2 0.2321 Tier II
10 NC_000075.6 9 41999647 - Sorl1 NNNGCTTGGAAAATCAGAGG NGG 2 0.2321 Tier II
11 NC_000074.6 8 122659917 - Cbfa2t3 NNNGACTGGAATATCAGATG NGG 2 0.1905 Tier II
12 NC_000080.6 14 117407132 - Gpc6 NNNGCCTGAAAAATCAGAAG NGG 2 0.1837 Tier II
13 NC_000072.6 6 56074683 + Pde1c NNNGCCTGGAGAATCAGAGG NGG 2 0.1625 Tier II
14 NC_000069.6 3 85709712 + Fam160a1 NNNGCCTGAAAACTCAGATG NGG 2 0.1353 Tier II
15 NC_000076.6 10 89980208 + Anks1b NNNGCCTGGAAATTCAGATC NGG 2 0.1286 Tier II
16 NC_000070.6 4 20406376 - Nkain3 NNNGCCTGGAAAAACAGCTG NGG 2 0.1179 Tier II
17 NC_000071.6 5 22867245 + Lhfpl3 NNNGACTGGAAAATCAGCTG NGG 2 0.1088 Tier II
18 NC_000069.6 3 95884676 - BC028528 NNNGCCTGGAAAATCAGAGC NGG 2 0.1071 Tier II
19 NC_000067.6 1 70710797 - Spag16 NNNGCCTGGCAAATCAGAAG NGG 2 0.0952 Tier II
20 NC_000082.6 16 41219223 + Lsamp NNNGCCTGGAAAATAAGATG NAG 2 0.0576 Tier II
21 NC_000074.6 8 83185160 - Tbc1d9 NNNGCCTGGAAAATCAGATT NTG 2 0.0273 Tier II
22 NC_000077.6 11 57311274 - Gria1 NNNGCCTGGAAAATGAGATG NAG 2 0.013 Tier II
23 NC_000067.6 1 162085695 - Dnm3 NNNGCCTGGAAAATCAGAGG NTG 2 0.0097 Tier II
24 NC_000072.6 6 121016012 + Mical3 NNNGCCTGGAAAACCAGATG NGT 2 0.0046 Tier II
25 NC_000076.6 10 11349639 + Epm2a NNNGCCTGGAAAATCTGATG NGC 2 0.0 Tier II
26 NC_000068.7 2 59401982 + Gm46743 NNNGCCTGGAAGATCAGATA NGG 2 0.6771 Tier III
27 NC_000072.6 6 5568107 + Gm34470 NNNGCATGGAAAAACAGATG NGG 2 0.5748 Tier III
28 NC_000071.6 5 53949751 - Gm40305 NNNCCCTGGACAATCAGATG NGG 2 0.2118 Tier III
29 NC_000079.6 13 28409340 + Gm40841 NNNGCCTGGAAAGTCAGATG NAG 2 0.1691 Tier III
30 NC_000069.6 3 95884676 - C920021L13Rik NNNGCCTGGAAAATCAGAGC NGG 2 0.1071 Tier III
31 NC_000083.6 17 94828010 + Gm1976 NNNGCCTGGAAAATATGATG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)