Construct: sgRNA BRDN0001147195
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCGGGCAGTGAGTACAATAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80232
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
101899688 |
+ |
BANK1 |
NNNGGCTGTGAGTACAAAAC |
NGG |
2 |
0.2917 |
Tier II |
| 2 |
NC_000004.12 |
4 |
105831078 |
+ |
GSTCD |
NNNGGCAGTGTGTACAAAAC |
NGG |
2 |
0.2051 |
Tier II |
| 3 |
NC_000005.10 |
5 |
61455708 |
+ |
ZSWIM6 |
NNNGGCAGTGAGGGCAATAC |
NGG |
2 |
0.1217 |
Tier II |
| 4 |
NC_000010.11 |
10 |
49302653 |
- |
C10orf71 |
NNNGGCAGTGAGTACAAGAC |
NTG |
2 |
0.013 |
Tier II |
| 5 |
NC_000002.12 |
2 |
73250248 |
+ |
CCT7 |
NNNGGCAGTGAGGACAATAC |
NGT |
2 |
0.0042 |
Tier II |
| 6 |
NC_000004.12 |
4 |
15131130 |
+ |
C1QTNF7-AS1 |
NNNGGCAGTGAGAACAATAT |
NGG |
2 |
0.2077 |
Tier III |
| 7 |
NC_000004.12 |
4 |
143045787 |
+ |
LOC100287014 |
NNNGGCAGTGAGTACAATGC |
NAG |
2 |
0.0972 |
Tier III |
| 8 |
NC_000004.12 |
4 |
143045787 |
+ |
USP38-DT |
NNNGGCAGTGAGTACAATGC |
NAG |
2 |
0.0972 |
Tier III |
| 9 |
NC_000011.10 |
11 |
87768783 |
+ |
LOC107984361 |
NNNGGCAGTGAGAACAATAC |
NTG |
2 |
0.027 |
Tier III |
| 10 |
NC_000007.14 |
7 |
38247719 |
- |
TRG |
NNNGGCAGTGAATACCATAC |
NGG |
2 |
0.0 |
Tier III |
| 11 |
NC_000007.14 |
7 |
38247719 |
- |
TRGC2 |
NNNGGCAGTGAATACCATAC |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
127582752 |
+ |
Phkg2 |
NNNGGCAGTGTGGACAATAC |
NGG |
2 |
0.0803 |
Tier I |
| 2 |
NC_000080.6 |
14 |
115369977 |
- |
Gpc5 |
NNNGGCAGTGAGGACAATAC |
NGC |
2 |
0.0058 |
Tier I |
| 3 |
NC_000070.6 |
4 |
146528254 |
- |
Zfp981 |
NNNGGCAGTGAATACAAAAC |
NGG |
2 |
0.6222 |
Tier II |
| 4 |
NC_000070.6 |
4 |
146528254 |
- |
Zfp993 |
NNNGGCAGTGAATACAAAAC |
NGG |
2 |
0.6222 |
Tier II |
| 5 |
NC_000085.6 |
19 |
55504289 |
- |
Vti1a |
NNNGGCATGGAGTACAATAC |
NGG |
2 |
0.3869 |
Tier II |
| 6 |
NC_000071.6 |
5 |
92604432 |
- |
Stbd1 |
NNNGGCAGTGAATACAAGAC |
NGG |
2 |
0.3111 |
Tier II |
| 7 |
NC_000079.6 |
13 |
108662172 |
- |
Pde4d |
NNNGGCAGTGAGAACAACAC |
NGG |
2 |
0.2967 |
Tier II |
| 8 |
NC_000069.6 |
3 |
27893322 |
- |
Tmem212 |
NNNGTCAGTGAATACAATAC |
NGG |
2 |
0.28 |
Tier II |
| 9 |
NC_000083.6 |
17 |
13879298 |
- |
Afdn |
NNNGTCAGAGAGTACAATAC |
NGG |
2 |
0.2786 |
Tier II |
| 10 |
NC_000076.6 |
10 |
120827082 |
+ |
Msrb3 |
NNNGGAAGTGAGTAAAATAC |
NGG |
2 |
0.2063 |
Tier II |
| 11 |
NC_000082.6 |
16 |
34341333 |
- |
Kalrn |
NNNGGCAGTGAATACACTAC |
NGG |
2 |
0.1647 |
Tier II |
| 12 |
NC_000080.6 |
14 |
35328958 |
+ |
Grid1 |
NNNGGAAGTGAGTACAGTAC |
NGG |
2 |
0.1639 |
Tier II |
| 13 |
NC_000085.6 |
19 |
17067083 |
+ |
Prune2 |
NNNGGCAGTGAGTTCAATAT |
NGG |
2 |
0.16 |
Tier II |
| 14 |
NC_000071.6 |
5 |
150303436 |
- |
Fry |
NNNGGCAGGGAGTACAATCC |
NGG |
2 |
0.1281 |
Tier II |
| 15 |
NC_000073.6 |
7 |
127582752 |
+ |
Ccdc189 |
NNNGGCAGTGTGGACAATAC |
NGG |
2 |
0.0803 |
Tier II |
| 16 |
NC_000069.6 |
3 |
50495193 |
- |
Slc7a11 |
NNNGGCAGTGAGTACACTAC |
NGT |
2 |
0.0028 |
Tier II |
| 17 |
NC_000072.6 |
6 |
71961484 |
- |
Polr1a |
NNNGGCAGTGAGTACCATGC |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000067.6 |
1 |
93815600 |
+ |
Ing5 |
NNNGGCAGTGTGTACCATAC |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000070.6 |
4 |
146528254 |
- |
1700095A21Rik |
NNNGGCAGTGAATACAAAAC |
NGG |
2 |
0.6222 |
Tier III |
| 20 |
NC_000070.6 |
4 |
146528254 |
- |
Vmn2r-ps16 |
NNNGGCAGTGAATACAAAAC |
NGG |
2 |
0.6222 |
Tier III |
| 21 |
NC_000072.6 |
6 |
135531128 |
+ |
Gm25136 |
NNNGGCAGTGACTACCATAC |
NGG |
2 |
0.0 |
Tier III |
| 22 |
NC_000072.6 |
6 |
71961484 |
- |
Gm26628 |
NNNGGCAGTGAGTACCATGC |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
