Construct: sgRNA BRDN0001147231

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGAAGCTCAGAAAGCCACGC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3BP5 (9467)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76846

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 15269853 - SH3BP5 NNNAGCTCAGAAAGCCACGC NGG 0 1.0 Tier I
2 NC_000009.12 9 122553717 - OR1N2 NNNGGCACAGAAAGCCACGC NGG 2 0.5469 Tier I
3 NC_000016.10 16 74630782 - RFWD3 NNNAGCTCAGAAAGCCAGGT NGG 2 0.04 Tier I
4 NC_000009.12 9 122553717 - OR1J2 NNNGGCACAGAAAGCCACGC NGG 2 0.5469 Tier II
5 NC_000009.12 9 122553717 - OR1L8 NNNGGCACAGAAAGCCACGC NGG 2 0.5469 Tier II
6 NC_000007.14 7 150959797 + KCNH2 NNNAGAGCAGAAAGCCACGC NGG 2 0.5462 Tier II
7 NC_000022.11 22 43172627 + TTLL12 NNNAGCACACAAAGCCACGC NGG 2 0.35 Tier II
8 NC_000020.11 20 9094848 - PLCB4 NNNAGCTAAGAAAGCCACGA NGG 2 0.325 Tier II
9 NC_000017.11 17 8096305 + ALOXE3 NNNAGCTCAGAAACCCAAGC NGG 2 0.2308 Tier II
10 NC_000001.11 1 43999266 + SLC6A9 NNNAGCCCAGAAAGCCAGGC NGG 2 0.0917 Tier II
11 NC_000009.12 9 98118581 - TRIM14 NNNAGCTCAGAAAGCCACAC NGA 2 0.0496 Tier II
12 NC_000016.10 16 50618777 - NKD1 NNNAGCTCAGAAAGCCACAC NGA 2 0.0496 Tier II
13 NC_000016.10 16 72208010 + PMFBP1 NNNAGCTCAGATAGCCAGGC NGG 2 0.0444 Tier II
14 NC_000023.11 X 108339179 + COL4A6 NNNAGCTCAGAAAGCCAGGC NAG 2 0.0346 Tier II
15 NC_000008.11 8 41719301 + ANK1 NNNAGCTCAGAAAGCCACGC NGC 1 0.0222 Tier II
16 NC_000011.10 11 125426948 + PKNOX2 NNNAGCTCAGAAAGGCACGC NAG 2 0.013 Tier II
17 NC_000001.11 1 14927740 + KAZN NNNAGCTCAGAAAGCCACGT NGC 2 0.0067 Tier II
18 NC_000012.12 12 96492308 - CFAP54 NNNAGCTCAGAAAGGCAGGC NGG 2 0.0067 Tier II
19 NC_000012.12 12 130504594 + RIMBP2 NNNAGCTCAGAAAGGCACGC NGT 2 0.0008 Tier II
20 NC_000022.11 22 18031831 - LINC01634 NNNAGCCCAGGAAGCCACGC NGG 2 0.4469 Tier III
21 NC_000009.12 9 11397640 + LOC105375974 NNNAGCTCCGAAAGCCACTC NGG 2 0.381 Tier III
22 NC_000014.9 14 46334185 + LINC00871 NNNAGCTCAGAAAGCCTCCC NGG 2 0.0598 Tier III
23 NC_000016.10 16 72208010 + LOC105371341 NNNAGCTCAGATAGCCAGGC NGG 2 0.0444 Tier III
24 NC_000013.11 13 105606853 + LOC105370345 NNNAGCTCAGAAAGCCCCGC NGT 2 0.0028 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 36907453 - Olfr354 NNNAGCACAGAAAGCCACAC NGG 2 0.625 Tier I
2 NC_000077.6 11 3367484 + Limk2 NNNACCTCAGAAAGCCACAC NGG 2 0.5612 Tier II
3 NC_000071.6 5 147315522 + Urad NNNAGATCAGAAAGCCAAGC NGG 2 0.5 Tier II
4 NC_000085.6 19 46621774 - Wbp1l NNNAGCCCAGAGAGCCACGC NGG 2 0.4965 Tier II
5 NC_000079.6 13 49144683 + Wnk2 NNNTGCTCAGAGAGCCACGC NGG 2 0.4596 Tier II
6 NC_000082.6 16 58459159 - Dcbld2 NNNAGCCCAGAAAGCCATGC NGG 2 0.442 Tier II
7 NC_000076.6 10 81049092 + Sgta NNNAGGACAGAAAGCCACGC NGG 2 0.4375 Tier II
8 NC_000073.6 7 27198052 + BC024978 NNNAGCTCAGGAAGCCAAGC NGG 2 0.35 Tier II
9 NC_000077.6 11 96074811 + Atp5g1 NNNCGCTCAGAAAGCCACGA NGG 2 0.1765 Tier II
10 NC_000083.6 17 11330842 - Prkn NNNAGCTCAGGAATCCACGC NGG 2 0.1733 Tier II
11 NC_000071.6 5 63920244 + Rell1 NNNAGCTCAGAAACACACGC NGG 2 0.0952 Tier II
12 NC_000077.6 11 115979885 + Itgb4 NNNAGCTCAGGAAGCCACGC NCG 2 0.0696 Tier II
13 NC_000068.7 2 157340082 + Ghrh NNNAGCTCAGAAAGCCAAGC NGA 2 0.0374 Tier II
14 NC_000072.6 6 32499267 - Plxna4 NNNAGCTCAGACAGCCAGGC NGG 2 0.0351 Tier II
15 NC_000075.6 9 121037342 + Ulk4 NNNAGCTCAGAAAGCCAGGC NCG 2 0.0143 Tier II
16 NC_000085.6 19 48392675 - Sorcs3 NNNAGCTCAGAAAGGCAGGC NGG 2 0.0067 Tier II
17 NC_000073.6 7 124027124 + Hs3st4 NNNAGCTCAGAAAGGCACGC NGC 2 0.0011 Tier II
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Other clones with same target sequence:

(none)