Construct: sgRNA BRDN0001147411

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCTCAAGCCTACACTATCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGK494 (124923)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77171

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 28611590 - RSKR NNNCAAGCCTACACTATCTG NGG 0 1.0 Tier I
2 NC_000010.11 10 26246351 - GAD2 NNNCAAGCTTACACTATCTA NGG 2 0.8203 Tier II
3 NC_000012.12 12 14657476 - GUCY2C NNNCCAACCTACACTATCTG NGG 2 0.5 Tier II
4 NC_000007.14 7 37686674 - GPR141 NNNCAAGCCTTCACTATCTA NGG 2 0.2885 Tier II
5 NC_000012.12 12 107328124 + ABTB3 NNNCAAGTCTACACTATCAG NGG 2 0.25 Tier II
6 NC_000021.9 21 25710638 + JAM2 NNNCAAGCCTAGAATATCTG NGG 2 0.1556 Tier II
7 NC_000017.11 17 63296921 + TANC2 NNNCAAGCCTACAAGATCTG NGG 2 0.0175 Tier II
8 NC_000001.11 1 174850767 + RABGAP1L NNNCAAGCCTACATTATCTG NGT 2 0.0118 Tier II
9 NC_000017.11 17 28611590 - SPAG5-AS1 NNNCAAGCCTACACTATCTG NGG 0 1.0 Tier III
10 NC_000001.11 1 42962535 + SLC2A1-DT NNNCAAGCCTACTCTACCTG NGG 2 0.2118 Tier III
11 NC_000021.9 21 25710638 + LOC124905002 NNNCAAGCCTAGAATATCTG NGG 2 0.1556 Tier III
12 NC_000013.11 13 104794919 - LOC107984606 NNNCAAGCCTACAATTTCTG NGG 2 0.0 Tier III
13 NC_000003.12 3 169954153 + FHL1P1 NNNCATGCCTACACTTTCTG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 78293596 + Rskr NNNCGAGCCTACACTATCTG NGG 1 0.72 Tier I
2 NC_000079.6 13 46509920 - Cap2 NNNCAAATCTACACTATCTG NGG 2 0.875 Tier II
3 NC_000068.7 2 175125767 + Gm14393 NNNCAAGCTTAAACTATCTG NGG 2 0.625 Tier II
4 NC_000068.7 2 177941360 + Gm14326 NNNCAAGCTTAAACTATCTG NGG 2 0.625 Tier II
5 NC_000074.6 8 104245946 + Tk2 NNNCAAGCCTCCACTATCTA NGG 2 0.375 Tier II
6 NC_000076.6 10 103052221 - Lrriq1 NNNCAAGTCTTCACTATCTG NGG 2 0.2692 Tier II
7 NC_000075.6 9 16099874 - Fat3 NNNCAAGCCTACACTATCTA NAG 2 0.2431 Tier II
8 NC_000081.6 15 100340182 + Mettl7a3 NNNCAAGACTACACTATCAG NGG 2 0.1857 Tier II
9 NC_000076.6 10 63506348 - Ctnna3 NNNCAGGCCTACACTATCTG NAG 2 0.1852 Tier II
10 NC_000086.7 X 164419951 + Piga NNNGAAGCCTACTCTATCTG NGG 2 0.15 Tier II
11 NC_000079.6 13 99906803 - Cartpt NNNCAAGCCTACTCTATCTC NGG 2 0.1286 Tier II
12 NC_000083.6 17 66878989 - Ptprm NNNCCAGCCTACCCTATCTG NGG 2 0.1053 Tier II
13 NC_000086.7 X 23780318 - Wdr44 NNNAAAGCCTACACTAGCTG NGG 2 0.0991 Tier II
14 NC_000071.6 5 92487848 + Scarb2 NNNCAAGCCTAAACTAGCTG NGG 2 0.084 Tier II
15 NC_000071.6 5 140632734 + Ttyh3 NNNCAAGCCTCCACTGTCTG NGG 2 0.0769 Tier II
16 NC_000072.6 6 136713392 - Gucy2c NNNCAAGCCTACACCGTCTG NGG 2 0.0524 Tier II
17 NC_000069.6 3 97252862 - Bcl9 NNNCCAGCCTACACTATCTG NGA 2 0.0347 Tier II
18 NC_000074.6 8 41097678 - Mtus1 NNNCAAGCCTACACTATCTT NTG 2 0.0273 Tier II
19 NC_000077.6 11 78293596 + Gm3948 NNNCGAGCCTACACTATCTG NGG 1 0.72 Tier III
20 NC_000082.6 16 27862411 - Gm10823 NNNCAAGGCTACACAATCTG NGG 2 0.3722 Tier III
21 NC_000085.6 19 21582763 - Gm38486 NNNAAAGCCTACACTAGCTG NGG 2 0.0991 Tier III
22 NC_000071.6 5 133549477 + Gm36667 NNNCTAGCCTACACTATCTG NGA 2 0.0253 Tier III
23 NC_000068.7 2 127101944 - Gm39922 NNNAAAGCCTACACTATCTG NGT 2 0.0136 Tier III
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Other clones with same target sequence:

(none)