Construct: sgRNA BRDN0001147530

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CATAGCCATTCAGCAGAAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK11A (728642)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75892

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 1652444 - CDK11B NNNAGCCATTCAGCAGAAGG NGG 0 1.0 Tier I
2 NC_000001.11 1 1719333 - CDK11A NNNAGCCATTCAGCAGAAGG NGG 0 1.0 Tier I
3 NC_000001.11 1 155676646 - YY1AP1 NNNAGCCAAACAGCAGAAGG NGG 2 0.7959 Tier I
4 NC_000001.11 1 111563919 - TMIGD3 NNNACCCATTGAGCAGAAGG NGG 2 0.1964 Tier I
5 NC_000022.11 22 36289168 - MYH9 NNNAGCCATGGAGCAGAAGG NGG 2 0.125 Tier I
6 NC_000014.9 14 101997174 + DYNC1H1 NNNTGCCATTCAGCAGCAGG NGG 2 0.1123 Tier I
7 NC_000011.10 11 28211117 + METTL15 NNNAGCCATTCTGCAGAAGG NGT 2 0.0054 Tier I
8 NC_000002.12 2 176156955 - HOXD3 NNNAGCCATTAAGCAGAAGA NGG 2 0.7031 Tier II
9 NC_000014.9 14 69966644 - SMOC1 NNNAGCAATTAAGCAGAAGG NGG 2 0.5625 Tier II
10 NC_000001.11 1 109302869 - MYBPHL NNNAGCCATCCAGCAAAAGG NGG 2 0.5333 Tier II
11 NC_000016.10 16 70855478 - HYDIN NNNAGCCACTCAGCAGAAGC NGG 2 0.3956 Tier II
12 NC_000012.12 12 103128014 - C12orf42 NNNAGCCATTCAGAAAAAGG NGG 2 0.35 Tier II
13 NC_000008.11 8 142346059 - TSNARE1 NNNTGGCATTCAGCAGAAGG NGG 2 0.3182 Tier II
14 NC_000012.12 12 63927441 + SRGAP1 NNNAGCCAGTCAGCAGATGG NGG 2 0.3095 Tier II
15 NC_000002.12 2 134300378 - MGAT5 NNNAGACATTCAGCAGAAGG NAG 2 0.2407 Tier II
16 NC_000004.12 4 5371204 + STK32B NNNAGCCAATGAGCAGAAGG NGG 2 0.2321 Tier II
17 NC_000013.11 13 45831391 + SIAH3 NNNAGGCTTTCAGCAGAAGG NGG 2 0.2143 Tier II
18 NC_000001.11 1 111563919 - RAP1A NNNACCCATTGAGCAGAAGG NGG 2 0.1964 Tier II
19 NC_000010.11 10 126991974 + DOCK1 NNNAGCCATTCAGCAGAAAG NAG 2 0.1852 Tier II
20 NC_000007.14 7 151496061 + RHEB NNNAGGCATTCTGCAGAAGG NGG 2 0.1667 Tier II
21 NC_000006.12 6 110225135 - CDC40 NNNATCCATCCAGCAGAAGG NGG 2 0.16 Tier II
22 NC_000001.11 1 12721649 - AADACL3 NNNAGGCATTTAGCAGAAGG NGG 2 0.1538 Tier II
23 NC_000001.11 1 40060026 - CAP1 NNNAGCCATGCATCAGAAGG NGG 2 0.15 Tier II
24 NC_000003.12 3 124281924 - KALRN NNNAACCATTCAGCAGTAGG NGG 2 0.1156 Tier II
25 NC_000022.11 22 33676263 + LARGE1 NNNAGCCAGTCAGCAGCAGG NGG 2 0.1092 Tier II
26 NC_000006.12 6 133995669 - SLC2A12 NNNAGCCATCCAGCTGAAGG NGG 2 0.1067 Tier II
27 NC_000006.12 6 5509379 - FARS2 NNNAGCCATTCACCAGACGG NGG 2 0.0802 Tier II
28 NC_000020.11 20 53403859 - TSHZ2 NNNATCCATTCAGCAGAAGG NAG 2 0.0778 Tier II
29 NC_000008.11 8 1853522 - ARHGEF10 NNNAGCCATTCAGCTGAAGG NGA 2 0.0139 Tier II
30 NC_000019.10 19 48127783 + LIG1 NNNAGCCATTCTGCAGAAGG NTG 2 0.013 Tier II
31 NC_000014.9 14 72398830 + RGS6 NNNAGCCATTCAGCAGCAGG NTG 2 0.0069 Tier II
32 NC_000010.11 10 69956653 - COL13A1 NNNAGCCACTCAGCACAAGG NGG 2 0.0 Tier II
33 NC_000014.9 14 71391997 + SIPA1L1 NNNAGCCATTCAGCATCAGG NGG 2 0.0 Tier II
34 NC_000003.12 3 119132952 - IGSF11 NNNAGCCATTCAGGAGAAGG NTG 2 0.0 Tier II
35 NC_000006.12 6 56371728 + COL21A1 NNNAGCCATTCAGGAGAAGT NGG 2 0.0 Tier II
36 NC_000004.12 4 113012168 + ANK2 NNNAGCCATTCAGGGGAAGG NGG 2 0.0 Tier II
37 NC_000008.11 8 116672512 + EIF3H NNNAGCTATTCAGGAGAAGG NGG 2 0.0 Tier II
38 NC_000001.11 1 73171853 - LOC105378800 NNNACCCTTTCAGCAGAAGG NGG 2 0.3367 Tier III
39 NC_000002.12 2 234694498 + LINC01173 NNNAGCCACTGAGCAGAAGG NGG 2 0.2308 Tier III
40 NC_000015.10 15 32213880 - LOC102724078 NNNAGCCTTTCACCAGAAGG NGG 2 0.1805 Tier III
41 NC_000018.10 18 41485763 - KC6 NNNAGCCATTCAGCAGTATG NGG 2 0.0889 Tier III
42 NC_000011.10 11 105273756 - LOC105369468 NNNAGCCATACAGCAGAAGG NTG 2 0.0334 Tier III
43 NC_000014.9 14 72398830 + LOC105370559 NNNAGCCATTCAGCAGCAGG NTG 2 0.0069 Tier III
44 NC_000003.12 3 156714604 - METTL15P1 NNNAGCCATTCTGCAGAAGG NGT 2 0.0054 Tier III
45 NC_000010.11 10 69956653 - LOC107984242 NNNAGCCACTCAGCACAAGG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 103893705 + Olfr64 NNNAGCCAGTCAGCAGAAAG NGG 2 0.4422 Tier I
2 NC_000070.6 4 155628819 + Cdk11b NNNAGCCATTCAGCAGAGGG NGG 1 0.4 Tier I
3 NC_000084.6 18 12199404 + Npc1 NNNAGTCCTTCAGCAGAAGG NGG 2 0.398 Tier I
4 NC_000074.6 8 83724941 + Adgre5 NNNTGCCATCCAGCAGAAGG NGG 2 0.3394 Tier I
5 NC_000082.6 16 88884820 + Krtap19-4 NNNAGCCTTTCAGTAGAAGG NGG 2 0.3143 Tier I
6 NC_000069.6 3 95294944 + Mindy1 NNNAGCCATGGAGCAGAAGG NGG 2 0.125 Tier I
7 NC_000069.6 3 114994614 - Olfm3 NNNAGCCATACAGCAAAAGG NGG 2 0.8571 Tier II
8 NC_000084.6 18 23553114 + Dtna NNNAGTCATTCAGCAGAATG NGG 2 0.619 Tier II
9 NC_000076.6 10 86261873 + Syn3 NNNTGCCATTCAACAGAAGG NGG 2 0.5874 Tier II
10 NC_000070.6 4 115123444 + Cyp4x1 NNNAAGCATTCAGCAGAAGG NGG 2 0.4333 Tier II
11 NC_000075.6 9 42804023 + Grik4 NNNACCCATTCAGCAGATGG NGG 2 0.3929 Tier II
12 NC_000068.7 2 166283063 - Gm30733 NNNGGCCAGTCAGCAGAAGG NGG 2 0.3869 Tier II
13 NC_000069.6 3 5332006 - Zfhx4 NNNAGCCATCCAGCAGAAGT NGG 2 0.3733 Tier II
14 NC_000074.6 8 83724941 + Ddx39a NNNTGCCATCCAGCAGAAGG NGG 2 0.3394 Tier II
15 NC_000082.6 16 20715182 - Clcn2 NNNAGCCATTCACCAGAATG NGG 2 0.2807 Tier II
16 NC_000067.6 1 132011065 - Elk4 NNNAGGCATCCAGCAGAAGG NGG 2 0.2667 Tier II
17 NC_000075.6 9 51166140 - Gm32742 NNNAGCCATTCATCAGAAAG NGG 2 0.2143 Tier II
18 NC_000074.6 8 122113099 + Zfp469 NNNAGCCATTCACCAGATGG NGG 2 0.2105 Tier II
19 NC_000084.6 18 80301742 + Kcng2 NNNAGCCACTCAGCTGAAGG NGG 2 0.1846 Tier II
20 NC_000079.6 13 42172121 - Hivep1 NNNAGACATTCAGCAGCAGG NGG 2 0.1639 Tier II
21 NC_000075.6 9 77310835 + Mlip NNNAGCCTTTCAGAAGAAGG NGG 2 0.15 Tier II
22 NC_000067.6 1 36747413 - 4933424G06Rik NNNAGCCATGCAGCAGAAGG NAG 2 0.1296 Tier II
23 NC_000070.6 4 136772802 + Ephb2 NNNAGCCATTTTGCAGAAGG NGG 2 0.1026 Tier II
24 NC_000077.6 11 113884725 + Sdk2 NNNAGCCATGCAGCTGAAGG NGG 2 0.1 Tier II
25 NC_000077.6 11 116387495 - Rnf157 NNNAGCCATCCAGCAGCAGG NGG 2 0.0941 Tier II
26 NC_000083.6 17 10766919 + Pacrg NNNAGCCATTCAGAAGCAGG NGG 2 0.0618 Tier II
27 NC_000070.6 4 106709410 - Mroh7 NNNAGCTATTCAGCAGAAGG NTG 2 0.0317 Tier II
28 NC_000081.6 15 81410030 - Xpnpep3 NNNAGCTATTCAGCAGAAGG NTG 2 0.0317 Tier II
29 NC_000074.6 8 108609956 - Zfhx3 NNNAGCCATTCAGAAGAAGG NGA 2 0.0243 Tier II
30 NC_000067.6 1 118457262 - Clasp1 NNNAGACATTCAGCACAAGG NGG 2 0.0 Tier II
31 NC_000068.7 2 6231995 - A230108P19Rik NNNAGCCATCCAACAGAAGG NGG 2 0.4923 Tier III
32 NC_000068.7 2 166283063 - Gm11468 NNNGGCCAGTCAGCAGAAGG NGG 2 0.3869 Tier III
33 NC_000086.7 X 74570874 - Olfr1326-ps1 NNNATCCATTCAGCAGAAGG NAG 2 0.0778 Tier III
34 NC_000069.6 3 54598932 - Gm40050 NNNAGCCATTTAGCAGGAGG NGG 2 0.0543 Tier III
35 NC_000069.6 3 143978168 + Gm33988 NNNAGCCATTCAGCAGCAGG NAG 2 0.0458 Tier III
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Other clones with same target sequence:

(none)