Construct: sgRNA BRDN0001147644
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATTGCCCAAACATCCACCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDKL1 (8814)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76269
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
50341141 |
+ |
CDKL1 |
NNNGCCCAAACATCCACCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
132699703 |
+ |
DNAAF11 |
NNNACCCAAACATCCACCAG |
NGG |
2 |
0.6429 |
Tier II |
| 3 |
NC_000005.10 |
5 |
138521092 |
+ |
ETF1 |
NNNACCCAAACATCCACTGG |
NGG |
2 |
0.5786 |
Tier II |
| 4 |
NC_000016.10 |
16 |
1166829 |
- |
CACNA1H |
NNNGCCCAGACATCCACCAG |
NGG |
2 |
0.4762 |
Tier II |
| 5 |
NC_000011.10 |
11 |
2420713 |
+ |
TRPM5 |
NNNGTCCAAACATCCACCAG |
NGG |
2 |
0.4545 |
Tier II |
| 6 |
NC_000017.11 |
17 |
79114525 |
+ |
RBFOX3 |
NNNGCCCACACATGCACCGG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000012.12 |
12 |
126994052 |
+ |
LINC02405 |
NNNCCCCAAACATCCTCCGG |
NGG |
2 |
0.0 |
Tier III |
| 8 |
NC_000012.12 |
12 |
126994052 |
+ |
LOC105370063 |
NNNCCCCAAACATCCTCCGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
126176159 |
+ |
Xpo6 |
NNNGCCCGAAAATCCACCGG |
NGG |
2 |
0.55 |
Tier II |
| 2 |
NC_000071.6 |
5 |
28171768 |
+ |
En2 |
NNNGTCCAAACATCCACCTG |
NGG |
2 |
0.4242 |
Tier II |
| 3 |
NC_000070.6 |
4 |
3773186 |
- |
Lyn |
NNNACCCAAATATCCACCGG |
NGG |
2 |
0.2769 |
Tier II |
| 4 |
NC_000071.6 |
5 |
65280170 |
+ |
Rfc1 |
NNNGCCCAAACATCCACTGG |
NGA |
2 |
0.0446 |
Tier II |
| 5 |
NC_000081.6 |
15 |
39061441 |
+ |
Gm7194 |
NNNGCCCACAAATCCACCGG |
NGG |
2 |
0.4286 |
Tier III |
| 6 |
NC_000086.7 |
X |
78624741 |
+ |
Gm8757 |
NNNGCCCACAAATCCACCGG |
NGG |
2 |
0.4286 |
Tier III |
| 7 |
NC_000086.7 |
X |
101824550 |
+ |
H3f3a-ps1 |
NNNGCCCACAAATCCACCGG |
NGG |
2 |
0.4286 |
Tier III |
Other clones with same target sequence:
(none)
