Construct: sgRNA BRDN0001147664
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGTCCAGGAAAAGTGTTTG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- PRKG1 (5592)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000010.11 | 10 | 51467786 | + | PRKG1 | NNNTCCAGGAAAAGTGTTTG | NGG | 0 | 1.0 | Tier I |
2 | NC_000005.10 | 5 | 150375730 | + | TCOF1 | NNNTCCAGGAAAAGTCCTTG | NGG | 2 | 0.0 | Tier I |
3 | NC_000010.11 | 10 | 95377092 | + | SORBS1 | NNNTCAGGGAAAAGTGTTTG | NGG | 2 | 0.6555 | Tier II |
4 | NC_000001.11 | 1 | 224556476 | - | CNIH3 | NNNTCCAGCAAAAGTGTTTG | NGG | 1 | 0.5385 | Tier II |
5 | NC_000002.12 | 2 | 219479475 | - | SPEG | NNNTCCAGGAGAAATGTTTG | NGG | 2 | 0.4875 | Tier II |
6 | NC_000007.14 | 7 | 55433687 | + | LANCL2 | NNNTCCACAAAAAGTGTTTG | NGG | 2 | 0.3956 | Tier II |
7 | NC_000003.12 | 3 | 186231193 | + | DGKG | NNNTCCAGGAAAAGTGATTT | NGG | 2 | 0.3733 | Tier II |
8 | NC_000022.11 | 22 | 25641713 | + | GRK3 | NNNTCCAGGAAAAGTGTGTG | NGG | 1 | 0.3333 | Tier II |
9 | NC_000019.10 | 19 | 35102910 | - | LOC124904701 | NNNTCCAGGAAGAGTGTTTC | NGG | 2 | 0.3095 | Tier II |
10 | NC_000014.9 | 14 | 78561674 | - | NRXN3 | NNNTCCAGGAAGAGTGTTCG | NGG | 2 | 0.1992 | Tier II |
11 | NC_000021.9 | 21 | 17585989 | - | CXADR | NNNTACAGGAAAAGTGTTAG | NGG | 2 | 0.1633 | Tier II |
12 | NC_000006.12 | 6 | 44298059 | + | POLR1C | NNNTCCAGGAAAAGCGTCTG | NGG | 2 | 0.1169 | Tier II |
13 | NC_000015.10 | 15 | 60620667 | + | RORA | NNNTCCAGGAGAAGTGTTTG | NGA | 2 | 0.0451 | Tier II |
14 | NC_000009.12 | 9 | 132793411 | - | AK8 | NNNTCCAGGAAAAGTGTTTG | NTG | 1 | 0.039 | Tier II |
15 | NC_000007.14 | 7 | 137636299 | + | DGKI | NNNTCCAGGATAAGTGGTTG | NGG | 2 | 0.0362 | Tier II |
16 | NC_000020.11 | 20 | 59002749 | - | CTSZ | NNNTCCAGGAAGAGGGTTTG | NGG | 2 | 0.0361 | Tier II |
17 | NC_000012.12 | 12 | 89491856 | - | POC1B | NNNTCCAGAAAAAGGGTTTG | NGG | 2 | 0.0321 | Tier II |
18 | NC_000006.12 | 6 | 168568392 | - | SMOC2 | NNNTCCAGGAAAAGTGGTGG | NGG | 2 | 0.0294 | Tier II |
19 | NC_000008.11 | 8 | 80647865 | + | ZNF704 | NNNTCCAGGAACAGGGTTTG | NGG | 2 | 0.0132 | Tier II |
20 | NC_000010.11 | 10 | 50471528 | - | SGMS1 | NNNACCAGGAAAAGTGTTTG | NGT | 2 | 0.0077 | Tier II |
21 | NC_000009.12 | 9 | 14205953 | - | NFIB | NNNTCCAGGAAAAGTGTGTG | NGT | 2 | 0.0054 | Tier II |
22 | NC_000001.11 | 1 | 216643672 | - | ESRRG | NNNTCCAGGAAAAGTCTTTA | NGG | 2 | 0.0 | Tier II |
23 | NC_000012.12 | 12 | 94640135 | + | TMCC3 | NNNTCCAGGAAAAGTCTTTG | NTG | 2 | 0.0 | Tier II |
24 | NC_000015.10 | 15 | 76701164 | - | SCAPER | NNNTCCAGGAAAAGTTATTG | NGG | 2 | 0.0 | Tier II |
25 | NC_000020.11 | 20 | 45707709 | + | WFDC13 | NNNTCCAGGAAAAGTTTTTG | NGG | 1 | 0.0 | Tier II |
26 | NC_000003.12 | 3 | 136471358 | + | STAG1 | NNNTCCAGGCAAAGTTTTTG | NGG | 2 | 0.0 | Tier II |
27 | NC_000006.12 | 6 | 166859616 | - | RPS6KA2 | NNNTCCAGGGAAAGTCTTTG | NGG | 2 | 0.0 | Tier II |
28 | NC_000001.11 | 1 | 105487495 | + | LOC105378881 | NNNTCCAGGAAAAGTATTTT | NGG | 2 | 0.7 | Tier III |
29 | NC_000008.11 | 8 | 117286742 | - | LOC105375716 | NNNTCCAGGAAAAGTGATTG | NGG | 1 | 0.5333 | Tier III |
30 | NC_000008.11 | 8 | 117286742 | - | LOC105375717 | NNNTCCAGGAAAAGTGATTG | NGG | 1 | 0.5333 | Tier III |
31 | NC_000009.12 | 9 | 1357329 | - | LOC102723803 | NNNTCCAGGAAAGGTGCTTG | NGG | 2 | 0.4604 | Tier III |
32 | NC_000020.11 | 20 | 22413310 | + | LOC284788 | NNNTCCAGGAAAAATGTCTG | NGG | 2 | 0.3214 | Tier III |
33 | NC_000019.10 | 19 | 35102910 | - | HPN-AS1 | NNNTCCAGGAAGAGTGTTTC | NGG | 2 | 0.3095 | Tier III |
34 | NC_000018.10 | 18 | 14556566 | - | LOC105372004 | NNNTCCAGGAAATGTATTTG | NGG | 2 | 0.3 | Tier III |
35 | NC_000009.12 | 9 | 97791191 | + | PTCSC2 | NNNTGGAGGAAAAGTGTTTG | NGG | 2 | 0.3 | Tier III |
36 | NC_000020.11 | 20 | 5473253 | + | LOC643406 | NNNTCCAGGAGAAGTGTCTG | NGG | 2 | 0.2786 | Tier III |
37 | NC_000020.11 | 20 | 5473253 | + | LOC107985411 | NNNTCCAGGAGAAGTGTCTG | NGG | 2 | 0.2786 | Tier III |
38 | NC_000013.11 | 13 | 106624233 | + | ARGLU1-DT | NNNTCCAGGAAATGTGTATG | NGG | 2 | 0.2 | Tier III |
39 | NC_000002.12 | 2 | 51872085 | + | NRXN1-DT | NNNTCCAGGATAATTGTTTG | NGG | 2 | 0.0821 | Tier III |
40 | NC_000015.10 | 15 | 60620667 | + | RORA-AS1 | NNNTCCAGGAGAAGTGTTTG | NGA | 2 | 0.0451 | Tier III |
41 | NC_000014.9 | 14 | 106086821 | + | IGH | NNNTCCAGGAAAAGGGCTTG | NGG | 2 | 0.0353 | Tier III |
42 | NC_000002.12 | 2 | 87326758 | - | LOC105374848 | NNNCCCAGGAAAAGTGTTTG | NTG | 2 | 0.0252 | Tier III |
43 | NC_000008.11 | 8 | 56498318 | - | LOC101929398 | NNNTCCAGAAAAAGTGTTTG | NGT | 2 | 0.0104 | Tier III |
44 | NC_000008.11 | 8 | 56498318 | - | PENK-AS1 | NNNTCCAGAAAAAGTGTTTG | NGT | 2 | 0.0104 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000085.6 | 19 | 31302337 | - | Prkg1 | NNNTCCTGGAAAAGTGTTCG | NGG | 2 | 0.1207 | Tier I |
2 | NC_000076.6 | 10 | 123337167 | - | Tafa2 | NNNTTCAAGAAAAGTGTTTG | NGG | 2 | 0.6364 | Tier II |
3 | NC_000073.6 | 7 | 84028723 | + | Cemip | NNNTCCAGGAAAAATGTTTT | NGG | 2 | 0.525 | Tier II |
4 | NC_000072.6 | 6 | 87709745 | - | 1810020O05Rik | NNNTGCAGGAAAAGTGCTTG | NGG | 2 | 0.4235 | Tier II |
5 | NC_000086.7 | X | 98298235 | - | Ar | NNNTTCAGAAAAAGTGTTTG | NGG | 2 | 0.4091 | Tier II |
6 | NC_000068.7 | 2 | 140677258 | + | Macrod2 | NNNTCTAGGAAAAGTGTGTG | NGG | 2 | 0.3095 | Tier II |
7 | NC_000077.6 | 11 | 107266421 | - | Pitpnc1 | NNNTTCAGGAAAAGTGTCTG | NGG | 2 | 0.2727 | Tier II |
8 | NC_000077.6 | 11 | 30823891 | + | Psme4 | NNNTACTGGAAAAGTGTTTG | NGG | 2 | 0.25 | Tier II |
9 | NC_000067.6 | 1 | 72490776 | + | Marchf4 | NNNTCTAGGAACAGTGTTTG | NGG | 2 | 0.2444 | Tier II |
10 | NC_000073.6 | 7 | 138373059 | - | Tcerg1l | NNNTCCTGTAAAAGTGTTTG | NGG | 2 | 0.2333 | Tier II |
11 | NC_000079.6 | 13 | 21528659 | - | Zkscan8 | NNNTCCAGAAATAGTGTTTG | NGG | 2 | 0.2143 | Tier II |
12 | NC_000074.6 | 8 | 70155512 | + | Mef2b | NNNTCCAGGAGCAGTGTTTG | NGG | 2 | 0.1711 | Tier II |
13 | NC_000074.6 | 8 | 70155512 | + | Gm45929 | NNNTCCAGGAGCAGTGTTTG | NGG | 2 | 0.1711 | Tier II |
14 | NC_000073.6 | 7 | 96209430 | + | Tenm4 | NNNCCCAGGAAAAGTGTTGG | NGG | 2 | 0.1618 | Tier II |
15 | NC_000076.6 | 10 | 83323853 | - | Slc41a2 | NNNTGCAGGAAACGTGTTTG | NGG | 2 | 0.1263 | Tier II |
16 | NC_000077.6 | 11 | 85785263 | + | Bcas3 | NNNTCCAGGAAAAGTGTCAG | NGG | 2 | 0.1224 | Tier II |
17 | NC_000067.6 | 1 | 8649166 | - | Sntg1 | NNNTCCAGGAAATGTGTGTG | NGG | 2 | 0.1 | Tier II |
18 | NC_000072.6 | 6 | 134755330 | - | Dusp16 | NNNTCCAGGAAAAGTGTGTG | NAG | 2 | 0.0864 | Tier II |
19 | NC_000077.6 | 11 | 4199244 | + | Tbc1d10a | NNNTCCAGGAACTGTGTTTG | NGG | 2 | 0.0789 | Tier II |
20 | NC_000067.6 | 1 | 84895178 | + | Fbxo36 | NNNTCCAGGAAGAGGGTTTG | NGG | 2 | 0.0361 | Tier II |
21 | NC_000073.6 | 7 | 40990825 | - | 4930433I11Rik | NNNTCCAGGACAAGTGTTTG | NGA | 2 | 0.0278 | Tier II |
22 | NC_000076.6 | 10 | 121563410 | - | Tbk1 | NNNTCCAGGGAAAGGGTTTG | NGG | 2 | 0.0278 | Tier II |
23 | NC_000075.6 | 9 | 9899372 | - | Cntn5 | NNNTCCAGGAGAAGTGTTTG | NGC | 2 | 0.0144 | Tier II |
24 | NC_000079.6 | 13 | 59506759 | - | Agtpbp1 | NNNTGCAGGAAAAGTGTTTG | NGC | 2 | 0.0133 | Tier II |
25 | NC_000073.6 | 7 | 112249434 | - | Mical2 | NNNTTCAGGAAAAGTGTTTG | NGT | 2 | 0.0103 | Tier II |
26 | NC_000068.7 | 2 | 54717503 | + | Galnt13 | NNNTCCTGGAAAAGTGTTTG | NGC | 2 | 0.0097 | Tier II |
27 | NC_000068.7 | 2 | 55026220 | + | Galnt13 | NNNTCCTGGAAAAGTGTTTG | NGC | 2 | 0.0097 | Tier II |
28 | NC_000071.6 | 5 | 112247625 | - | Cryba4 | NNNTCCAGGAAAAGAGTTTG | NGT | 2 | 0.0093 | Tier II |
29 | NC_000083.6 | 17 | 40567052 | - | Gm44501 | NNNTCCAGGAAAAGTGGTTG | NTG | 2 | 0.0046 | Tier II |
30 | NC_000081.6 | 15 | 58346649 | + | Anxa13 | NNNTCCAGGAAAAGCTTTTG | NGG | 2 | 0.0 | Tier II |
31 | NC_000077.6 | 11 | 72154430 | + | 4933427D14Rik | NNNTCCAGGAAAAGTCTTTT | NGG | 2 | 0.0 | Tier II |
32 | NC_000079.6 | 13 | 103193571 | - | Mast4 | NNNTCCAGGAAAAGTTTTTG | NCG | 2 | 0.0 | Tier II |
33 | NC_000076.6 | 10 | 125770610 | - | Gm29862 | NNNTCCAGGTAAAGTGTTTA | NGG | 2 | 0.8272 | Tier III |
34 | NC_000072.6 | 6 | 70116786 | + | Igk | NNNTCAAGGAAAAGAGTTTG | NGG | 2 | 0.5376 | Tier III |
35 | NC_000076.6 | 10 | 117230295 | - | Gm31963 | NNNTCCAGGAAAAGTGATTA | NGG | 2 | 0.5 | Tier III |
36 | NC_000073.6 | 7 | 129791866 | + | Gm33323 | NNNTCCATGAATAGTGTTTG | NGG | 2 | 0.2083 | Tier III |
37 | NC_000080.6 | 14 | 13213022 | + | Gm5087 | NNNTGCAGGCAAAGTGTTTG | NGG | 2 | 0.2 | Tier III |
38 | NC_000077.6 | 11 | 55947283 | - | LOC105244170 | NNNTCCAGGAAAGGTGTTGG | NGG | 2 | 0.163 | Tier III |
39 | NC_000073.6 | 7 | 7095327 | - | Gm6828 | NNNTGCAGGAAAAGTGTTTG | NAG | 2 | 0.1556 | Tier III |
40 | NC_000075.6 | 9 | 23035446 | - | Gm36743 | NNNTCCAGGGACAGTGTTTG | NGG | 2 | 0.1462 | Tier III |
41 | NC_000083.6 | 17 | 84938988 | - | 1110020A21Rik | NNNTCCAGGAGAAGGGTTTG | NGG | 2 | 0.0325 | Tier III |
42 | NC_000073.6 | 7 | 38766995 | + | Gm21276 | NNNTCCAGGACAAGTGTTTG | NGA | 2 | 0.0278 | Tier III |
43 | NC_000073.6 | 7 | 38905995 | + | Gm21142 | NNNTCCAGGACAAGTGTTTG | NGA | 2 | 0.0278 | Tier III |
44 | NC_000073.6 | 7 | 39261159 | + | Gm16387 | NNNTCCAGGACAAGTGTTTG | NGA | 2 | 0.0278 | Tier III |
45 | NC_000069.6 | 3 | 106284016 | + | Gm6522 | NNNTCCAGGGAAAGGGTTTG | NGG | 2 | 0.0278 | Tier III |
46 | NC_000075.6 | 9 | 69828110 | + | B230323A14Rik | NNNTCCAGGAAAAGTGTTTT | NGT | 2 | 0.0113 | Tier III |
47 | NC_000080.6 | 14 | 48855603 | + | Gm41149 | NNNTACAGGAAAAGTTTTTG | NGG | 2 | 0.0 | Tier III |
48 | NC_000074.6 | 8 | 128258899 | - | A930035F09Rik | NNNTCCATGAAAAGTCTTTG | NGG | 2 | 0.0 | Tier III |