Construct: sgRNA BRDN0001147755
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATTCCTAAAATGAAGGACA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- PDGFRL (5157)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000008.11 | 8 | 17589573 | + | PDGFRL | NNNTCCTAAAATGAAGGACA | NGG | 0 | 1.0 | Tier I |
2 | NC_000021.9 | 21 | 39265012 | - | BRWD1 | NNNTCCTAGATTGAAGGACA | NGG | 2 | 0.2051 | Tier I |
3 | NC_000009.12 | 9 | 132400107 | - | TTF1 | NNNTCCTAACATCAAGGACA | NGG | 2 | 0.1404 | Tier I |
4 | NC_000013.11 | 13 | 24831101 | - | RNF17 | NNNTCCTAAAATGAAAAACA | NGG | 2 | 0.9333 | Tier II |
5 | NC_000010.11 | 10 | 86280393 | - | GRID1 | NNNTCCTGAAATGAAGAACA | NGG | 2 | 0.6844 | Tier II |
6 | NC_000011.10 | 11 | 4952103 | - | MMP26 | NNNTCCTGAAATGAAGAACA | NGG | 2 | 0.6844 | Tier II |
7 | NC_000001.11 | 1 | 231196055 | - | TRIM67 | NNNTCCTAGAATGAAGGACA | NGG | 1 | 0.6667 | Tier II |
8 | NC_000008.11 | 8 | 32414962 | - | NRG1 | NNNCCCTAAAATGAAGGACA | NGG | 1 | 0.6471 | Tier II |
9 | NC_000001.11 | 1 | 183298141 | + | NMNAT2 | NNNCCCTAAAATGAAGAACA | NGG | 2 | 0.6039 | Tier II |
10 | NC_000006.12 | 6 | 124020402 | + | NKAIN2 | NNNTCCTAAAATGAAGATCA | NGG | 2 | 0.4667 | Tier II |
11 | NC_000003.12 | 3 | 20103131 | - | KAT2B | NNNTCCTAAAATGAAAGATA | NGG | 2 | 0.4615 | Tier II |
12 | NC_000009.12 | 9 | 83627687 | - | IDNK | NNNTCGTAAAATAAAGGACA | NGG | 2 | 0.4615 | Tier II |
13 | NC_000002.12 | 2 | 50738035 | + | NRXN1 | NNNTCCAAAAAGGAAGGACA | NGG | 2 | 0.4375 | Tier II |
14 | NC_000012.12 | 12 | 108552306 | + | SART3 | NNNTCCTAAAATCAAGAACA | NGG | 2 | 0.393 | Tier II |
15 | NC_000006.12 | 6 | 12296863 | - | EDN1 | NNNTCCAAAAATCAAGGACA | NGG | 2 | 0.3684 | Tier II |
16 | NC_000005.10 | 5 | 137961505 | + | FAM13B | NNNTCCTGAAAGGAAGGACA | NGG | 2 | 0.3667 | Tier II |
17 | NC_000020.11 | 20 | 2657917 | - | NOP56 | NNNTCCTAAAGGGAAGGACA | NGG | 2 | 0.325 | Tier II |
18 | NC_000004.12 | 4 | 185915367 | + | SORBS2 | NNNTCCTAAAATGAAGGTCT | NGG | 2 | 0.3 | Tier II |
19 | NC_000011.10 | 11 | 132478126 | + | OPCML | NNNTGCTAAAATGAAGGTCA | NGG | 2 | 0.3 | Tier II |
20 | NC_000007.14 | 7 | 135390370 | + | CNOT4 | NNNTCCTAAAATGAAGGAAT | NGG | 2 | 0.2571 | Tier II |
21 | NC_000001.11 | 1 | 31020742 | - | PUM1 | NNNTCCTATAATGAAGGAAA | NGG | 2 | 0.2571 | Tier II |
22 | NC_000004.12 | 4 | 114947979 | - | NDST4 | NNNTCATAAAATGAAGCACA | NGG | 2 | 0.2185 | Tier II |
23 | NC_000023.11 | X | 23841931 | - | APOO | NNNTGCTAAATTGAAGGACA | NGG | 2 | 0.1846 | Tier II |
24 | NC_000004.12 | 4 | 133181065 | - | PCDH10 | NNNTCCTAAAATGAAGGCCT | NGG | 2 | 0.1143 | Tier II |
25 | NC_000018.10 | 18 | 23392527 | - | TMEM241 | NNNTCCTCAAATGAAGGACA | NAG | 2 | 0.1111 | Tier II |
26 | NC_000018.10 | 18 | 80215141 | + | PARD6G | NNNTCCAAAAATGAAGGAGA | NGG | 2 | 0.1094 | Tier II |
27 | NC_000004.12 | 4 | 142409698 | - | INPP4B | NNNTCCTAAAATCAAGGACA | NAG | 2 | 0.1092 | Tier II |
28 | NC_000007.14 | 7 | 131180319 | + | MKLN1 | NNNTCCTAAAATGAAGCAAA | NGG | 2 | 0.1008 | Tier II |
29 | NC_000002.12 | 2 | 259052 | - | SH3YL1 | NNNTCCTAAACTGCAGGACA | NGG | 2 | 0.0857 | Tier II |
30 | NC_000004.12 | 4 | 85792330 | + | ARHGAP24 | NNNTCCTAAAATCAAGGCCA | NGG | 2 | 0.0802 | Tier II |
31 | NC_000011.10 | 11 | 100055790 | - | CNTN5 | NNNTCCTAAAATGAAGGACA | NGA | 1 | 0.0694 | Tier II |
32 | NC_000020.11 | 20 | 2327096 | - | TGM3 | NNNTCCTAAAATGATGGACA | NAG | 2 | 0.0519 | Tier II |
33 | NC_000003.12 | 3 | 57286766 | - | ASB14 | NNNTCCTAAAATGAAGGACA | NGT | 1 | 0.0161 | Tier II |
34 | NC_000004.12 | 4 | 53552247 | + | LNX1 | NNNTCCTAAAATGAAGGGCA | NGT | 2 | 0.0065 | Tier II |
35 | NC_000010.11 | 10 | 23074836 | - | LOC107984215 | NNNTCATAAAATGAAGGACA | NGG | 1 | 0.9286 | Tier III |
36 | NC_000002.12 | 2 | 116367280 | + | LOC105373576 | NNNTCCTAAAATAAAGGACA | NGG | 1 | 0.9231 | Tier III |
37 | NC_000013.11 | 13 | 91176300 | - | LINC00379 | NNNTCCAAAAATGAAGGACA | NGG | 1 | 0.875 | Tier III |
38 | NC_000002.12 | 2 | 18808493 | + | LOC105373456 | NNNTCCTAAAATAAAGAACA | NGG | 2 | 0.8615 | Tier III |
39 | NC_000013.11 | 13 | 85447350 | - | LINC00351 | NNNTCATAAAATAAAGGACA | NGG | 2 | 0.8571 | Tier III |
40 | NC_000004.12 | 4 | 185915367 | + | LOC101928929 | NNNTCCTAAAATGAAGGTCT | NGG | 2 | 0.3 | Tier III |
41 | NC_000009.12 | 9 | 68621751 | + | TMEM252-DT | NNNTCCTAAAATGAAGGAGG | NGG | 2 | 0.0956 | Tier III |
42 | NC_000011.10 | 11 | 100055790 | - | LOC105369456 | NNNTCCTAAAATGAAGGACA | NGA | 1 | 0.0694 | Tier III |
43 | NC_000019.10 | 19 | 58006230 | + | ZNF606-AS1 | NNNTCCTAAAATAAAGGACA | NTG | 2 | 0.036 | Tier III |
44 | NC_000014.9 | 14 | 48925936 | + | LOC105378178 | NNNTCCTAATATGAAGGACA | NTG | 2 | 0.0344 | Tier III |
45 | NC_000015.10 | 15 | 93430818 | - | SEPHS1P2 | NNNTCCTAAAATGACCGACA | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000068.7 | 2 | 31982641 | - | Nup214 | NNNTCATAAAATGGAGGACA | NGG | 2 | 0.4333 | Tier I |
2 | NC_000070.6 | 4 | 84598781 | + | Bnc2 | NNNTCTTAAAATGAAGGACA | NGG | 1 | 0.9286 | Tier II |
3 | NC_000086.7 | X | 69557569 | + | Aff2 | NNNTCTTAAAATGAAGGACA | NGG | 1 | 0.9286 | Tier II |
4 | NC_000076.6 | 10 | 27176513 | + | Lama2 | NNNTCTTAAAATGAAGAACA | NGG | 2 | 0.8667 | Tier II |
5 | NC_000086.7 | X | 10300976 | + | Otc | NNNTCCTAAAGTGAAGGACA | NGG | 1 | 0.65 | Tier II |
6 | NC_000072.6 | 6 | 104655850 | - | Cntn6 | NNNTTCTAAAATGAAGGACA | NGG | 1 | 0.6364 | Tier II |
7 | NC_000082.6 | 16 | 41351859 | - | Lsamp | NNNTGCTAAAATGAAAGACA | NGG | 2 | 0.6 | Tier II |
8 | NC_000071.6 | 5 | 101987932 | - | Wdfy3 | NNNTCCTAAGATGAAGGACA | NGG | 1 | 0.5556 | Tier II |
9 | NC_000086.7 | X | 53437077 | - | Etd | NNNTCTTAAAAGGAAGGACA | NGG | 2 | 0.4643 | Tier II |
10 | NC_000070.6 | 4 | 115466238 | - | Cyp4a30b | NNNTCCTAAAATAAAGGATA | NGG | 2 | 0.426 | Tier II |
11 | NC_000086.7 | X | 9291853 | - | Xk | NNNACCAAAAATGAAGGACA | NGG | 2 | 0.4167 | Tier II |
12 | NC_000085.6 | 19 | 55801868 | - | Tcf7l2 | NNNTGCTAAAGTGAAGGACA | NGG | 2 | 0.39 | Tier II |
13 | NC_000073.6 | 7 | 43300489 | + | Zfp715 | NNNTCCAAAAATGAAGGAAA | NGG | 2 | 0.375 | Tier II |
14 | NC_000069.6 | 3 | 152435921 | - | Zzz3 | NNNTCTTAAACTGAAGGACA | NGG | 2 | 0.3714 | Tier II |
15 | NC_000081.6 | 15 | 21145865 | - | Cdh12 | NNNTACTAAAATGAAGGACT | NGG | 2 | 0.3429 | Tier II |
16 | NC_000076.6 | 10 | 111979333 | - | Krr1 | NNNTGCTACAATGAAGGACA | NGG | 2 | 0.3429 | Tier II |
17 | NC_000068.7 | 2 | 97467738 | + | Lrrc4c | NNNTGCTAAAATGAAGGATA | NGG | 2 | 0.2769 | Tier II |
18 | NC_000070.6 | 4 | 112629395 | - | Skint2 | NNNTACTAAAATCAAGGACA | NGG | 2 | 0.2406 | Tier II |
19 | NC_000079.6 | 13 | 94836529 | + | Tbca | NNNTCCTAAAATGAAGCACA | NGG | 1 | 0.2353 | Tier II |
20 | NC_000068.7 | 2 | 142382647 | + | Macrod2 | NNNTCCTAAAATGATGGACA | NGG | 1 | 0.2 | Tier II |
21 | NC_000067.6 | 1 | 150921473 | + | Hmcn1 | NNNTCCCAAAATGAAGGACC | NGG | 2 | 0.1563 | Tier II |
22 | NC_000068.7 | 2 | 135935400 | - | Plcb4 | NNNTCCTAAAGTGAAGGACC | NGG | 2 | 0.1477 | Tier II |
23 | NC_000070.6 | 4 | 84342770 | + | Bnc2 | NNNTCCTAAAATGTAGGACA | NAG | 2 | 0.1383 | Tier II |
24 | NC_000077.6 | 11 | 77324329 | - | Ssh2 | NNNTCCTAAAAGGAAGTACA | NGG | 2 | 0.125 | Tier II |
25 | NC_000079.6 | 13 | 114152828 | - | Arl15 | NNNTCCTAAAATGAAGGAAA | NAG | 2 | 0.1111 | Tier II |
26 | NC_000084.6 | 18 | 75593014 | - | Ctif | NNNTCCTTAAATGAAGGACA | NAG | 2 | 0.1111 | Tier II |
27 | NC_000076.6 | 10 | 14428973 | + | Adgrg6 | NNNTCCTTAAATGAAGTACA | NGG | 2 | 0.1071 | Tier II |
28 | NC_000077.6 | 11 | 76088391 | - | Vps53 | NNNTCCTAAAATCAAGGACC | NGG | 2 | 0.0957 | Tier II |
29 | NC_000074.6 | 8 | 22414250 | - | Mrps31 | NNNTCCTCAAATGCAGGACA | NGG | 2 | 0.0918 | Tier II |
30 | NC_000071.6 | 5 | 34454910 | - | Fam193a | NNNTCCTAAAATGCAGCACA | NGG | 2 | 0.0504 | Tier II |
31 | NC_000083.6 | 17 | 68561449 | - | L3mbtl4 | NNNTCCTAAAATGCTGGACA | NGG | 2 | 0.0429 | Tier II |
32 | NC_000085.6 | 19 | 16262460 | + | Gnaq | NNNTCCTAAAATGAAGAACA | NTG | 2 | 0.0364 | Tier II |
33 | NC_000086.7 | X | 83462292 | - | Dmd | NNNTCCTAAATTGAAGGACA | NTG | 2 | 0.012 | Tier II |
34 | NC_000081.6 | 15 | 48364606 | - | Csmd3 | NNNTCCTAAAATGAAGTACA | NTG | 2 | 0.0097 | Tier II |
35 | NC_000078.6 | 12 | 89694683 | - | Nrxn3 | NNNTCCTAAAATCAAGGACA | NGC | 2 | 0.0094 | Tier II |
36 | NC_000078.6 | 12 | 9938616 | + | Gm33734 | NNNCCCTAAGATGAAGGACA | NGG | 2 | 0.3595 | Tier III |
37 | NC_000079.6 | 13 | 53761512 | - | Gm34439 | NNNTCCAAAAATGAAGGGCA | NGG | 2 | 0.35 | Tier III |
38 | NC_000079.6 | 13 | 9683342 | + | Gm36423 | NNNTCTTAACATGAAGGACA | NGG | 2 | 0.3095 | Tier III |
39 | NC_000071.6 | 5 | 28063764 | - | Gm35223 | NNNTCCTAAGATGAAGGTCA | NGG | 2 | 0.2778 | Tier III |
40 | NC_000076.6 | 10 | 97890529 | - | Gm10754 | NNNTCCTAATATTAAGGACA | NGG | 2 | 0.2647 | Tier III |
41 | NC_000068.7 | 2 | 50794782 | + | Gm32232 | NNNACGTAAAATGAAGGACA | NGG | 2 | 0.2381 | Tier III |
42 | NC_000075.6 | 9 | 92768654 | - | 4933400C23Rik | NNNTCCTAAAATGAGGGACC | NGG | 2 | 0.1477 | Tier III |
43 | NC_000068.7 | 2 | 167584247 | + | Gm31619 | NNNTCCAAAAATGAAGGAGA | NGG | 2 | 0.1094 | Tier III |
44 | NC_000068.7 | 2 | 167584247 | + | Gm11476 | NNNTCCAAAAATGAAGGAGA | NGG | 2 | 0.1094 | Tier III |