Construct: sgRNA BRDN0001147755

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AATTCCTAAAATGAAGGACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDGFRL (5157)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77698

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 17589573 + PDGFRL NNNTCCTAAAATGAAGGACA NGG 0 1.0 Tier I
2 NC_000021.9 21 39265012 - BRWD1 NNNTCCTAGATTGAAGGACA NGG 2 0.2051 Tier I
3 NC_000009.12 9 132400107 - TTF1 NNNTCCTAACATCAAGGACA NGG 2 0.1404 Tier I
4 NC_000013.11 13 24831101 - RNF17 NNNTCCTAAAATGAAAAACA NGG 2 0.9333 Tier II
5 NC_000010.11 10 86280393 - GRID1 NNNTCCTGAAATGAAGAACA NGG 2 0.6844 Tier II
6 NC_000011.10 11 4952103 - MMP26 NNNTCCTGAAATGAAGAACA NGG 2 0.6844 Tier II
7 NC_000001.11 1 231196055 - TRIM67 NNNTCCTAGAATGAAGGACA NGG 1 0.6667 Tier II
8 NC_000008.11 8 32414962 - NRG1 NNNCCCTAAAATGAAGGACA NGG 1 0.6471 Tier II
9 NC_000001.11 1 183298141 + NMNAT2 NNNCCCTAAAATGAAGAACA NGG 2 0.6039 Tier II
10 NC_000006.12 6 124020402 + NKAIN2 NNNTCCTAAAATGAAGATCA NGG 2 0.4667 Tier II
11 NC_000003.12 3 20103131 - KAT2B NNNTCCTAAAATGAAAGATA NGG 2 0.4615 Tier II
12 NC_000009.12 9 83627687 - IDNK NNNTCGTAAAATAAAGGACA NGG 2 0.4615 Tier II
13 NC_000002.12 2 50738035 + NRXN1 NNNTCCAAAAAGGAAGGACA NGG 2 0.4375 Tier II
14 NC_000012.12 12 108552306 + SART3 NNNTCCTAAAATCAAGAACA NGG 2 0.393 Tier II
15 NC_000006.12 6 12296863 - EDN1 NNNTCCAAAAATCAAGGACA NGG 2 0.3684 Tier II
16 NC_000005.10 5 137961505 + FAM13B NNNTCCTGAAAGGAAGGACA NGG 2 0.3667 Tier II
17 NC_000020.11 20 2657917 - NOP56 NNNTCCTAAAGGGAAGGACA NGG 2 0.325 Tier II
18 NC_000004.12 4 185915367 + SORBS2 NNNTCCTAAAATGAAGGTCT NGG 2 0.3 Tier II
19 NC_000011.10 11 132478126 + OPCML NNNTGCTAAAATGAAGGTCA NGG 2 0.3 Tier II
20 NC_000007.14 7 135390370 + CNOT4 NNNTCCTAAAATGAAGGAAT NGG 2 0.2571 Tier II
21 NC_000001.11 1 31020742 - PUM1 NNNTCCTATAATGAAGGAAA NGG 2 0.2571 Tier II
22 NC_000004.12 4 114947979 - NDST4 NNNTCATAAAATGAAGCACA NGG 2 0.2185 Tier II
23 NC_000023.11 X 23841931 - APOO NNNTGCTAAATTGAAGGACA NGG 2 0.1846 Tier II
24 NC_000004.12 4 133181065 - PCDH10 NNNTCCTAAAATGAAGGCCT NGG 2 0.1143 Tier II
25 NC_000018.10 18 23392527 - SLC35D4 NNNTCCTCAAATGAAGGACA NAG 2 0.1111 Tier II
26 NC_000018.10 18 80215141 + PARD6G NNNTCCAAAAATGAAGGAGA NGG 2 0.1094 Tier II
27 NC_000004.12 4 142409698 - INPP4B NNNTCCTAAAATCAAGGACA NAG 2 0.1092 Tier II
28 NC_000007.14 7 131180319 + MKLN1 NNNTCCTAAAATGAAGCAAA NGG 2 0.1008 Tier II
29 NC_000002.12 2 259052 - SH3YL1 NNNTCCTAAACTGCAGGACA NGG 2 0.0857 Tier II
30 NC_000004.12 4 85792330 + ARHGAP24 NNNTCCTAAAATCAAGGCCA NGG 2 0.0802 Tier II
31 NC_000011.10 11 100055790 - CNTN5 NNNTCCTAAAATGAAGGACA NGA 1 0.0694 Tier II
32 NC_000020.11 20 2327096 - TGM3 NNNTCCTAAAATGATGGACA NAG 2 0.0519 Tier II
33 NC_000003.12 3 57286766 - ASB14 NNNTCCTAAAATGAAGGACA NGT 1 0.0161 Tier II
34 NC_000004.12 4 53552247 + LNX1 NNNTCCTAAAATGAAGGGCA NGT 2 0.0065 Tier II
35 NC_000010.11 10 23074836 - LOC107984215 NNNTCATAAAATGAAGGACA NGG 1 0.9286 Tier III
36 NC_000002.12 2 116367280 + LOC105373576 NNNTCCTAAAATAAAGGACA NGG 1 0.9231 Tier III
37 NC_000013.11 13 91176300 - LINC00379 NNNTCCAAAAATGAAGGACA NGG 1 0.875 Tier III
38 NC_000002.12 2 18808493 + LOC105373456 NNNTCCTAAAATAAAGAACA NGG 2 0.8615 Tier III
39 NC_000013.11 13 85447350 - LINC00351 NNNTCATAAAATAAAGGACA NGG 2 0.8571 Tier III
40 NC_000004.12 4 185915367 + LOC101928929 NNNTCCTAAAATGAAGGTCT NGG 2 0.3 Tier III
41 NC_000009.12 9 68621751 + TMEM252-DT NNNTCCTAAAATGAAGGAGG NGG 2 0.0956 Tier III
42 NC_000011.10 11 100055790 - LOC105369456 NNNTCCTAAAATGAAGGACA NGA 1 0.0694 Tier III
43 NC_000019.10 19 58006230 + ZNF606-AS1 NNNTCCTAAAATAAAGGACA NTG 2 0.036 Tier III
44 NC_000014.9 14 48925936 + LOC105378178 NNNTCCTAATATGAAGGACA NTG 2 0.0344 Tier III
45 NC_000015.10 15 93430818 - SEPHS1P2 NNNTCCTAAAATGACCGACA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 31982641 - Nup214 NNNTCATAAAATGGAGGACA NGG 2 0.4333 Tier I
2 NC_000070.6 4 84598781 + Bnc2 NNNTCTTAAAATGAAGGACA NGG 1 0.9286 Tier II
3 NC_000086.7 X 69557569 + Aff2 NNNTCTTAAAATGAAGGACA NGG 1 0.9286 Tier II
4 NC_000076.6 10 27176513 + Lama2 NNNTCTTAAAATGAAGAACA NGG 2 0.8667 Tier II
5 NC_000086.7 X 10300976 + Otc NNNTCCTAAAGTGAAGGACA NGG 1 0.65 Tier II
6 NC_000072.6 6 104655850 - Cntn6 NNNTTCTAAAATGAAGGACA NGG 1 0.6364 Tier II
7 NC_000082.6 16 41351859 - Lsamp NNNTGCTAAAATGAAAGACA NGG 2 0.6 Tier II
8 NC_000071.6 5 101987932 - Wdfy3 NNNTCCTAAGATGAAGGACA NGG 1 0.5556 Tier II
9 NC_000086.7 X 53437077 - Etd NNNTCTTAAAAGGAAGGACA NGG 2 0.4643 Tier II
10 NC_000070.6 4 115466238 - Cyp4a30b NNNTCCTAAAATAAAGGATA NGG 2 0.426 Tier II
11 NC_000086.7 X 9291853 - Xk NNNACCAAAAATGAAGGACA NGG 2 0.4167 Tier II
12 NC_000085.6 19 55801868 - Tcf7l2 NNNTGCTAAAGTGAAGGACA NGG 2 0.39 Tier II
13 NC_000073.6 7 43300489 + Zfp715 NNNTCCAAAAATGAAGGAAA NGG 2 0.375 Tier II
14 NC_000069.6 3 152435921 - Zzz3 NNNTCTTAAACTGAAGGACA NGG 2 0.3714 Tier II
15 NC_000081.6 15 21145865 - Cdh12 NNNTACTAAAATGAAGGACT NGG 2 0.3429 Tier II
16 NC_000076.6 10 111979333 - Krr1 NNNTGCTACAATGAAGGACA NGG 2 0.3429 Tier II
17 NC_000068.7 2 97467738 + Lrrc4c NNNTGCTAAAATGAAGGATA NGG 2 0.2769 Tier II
18 NC_000070.6 4 112629395 - Skint2 NNNTACTAAAATCAAGGACA NGG 2 0.2406 Tier II
19 NC_000079.6 13 94836529 + Tbca NNNTCCTAAAATGAAGCACA NGG 1 0.2353 Tier II
20 NC_000068.7 2 142382647 + Macrod2 NNNTCCTAAAATGATGGACA NGG 1 0.2 Tier II
21 NC_000067.6 1 150921473 + Hmcn1 NNNTCCCAAAATGAAGGACC NGG 2 0.1563 Tier II
22 NC_000068.7 2 135935400 - Plcb4 NNNTCCTAAAGTGAAGGACC NGG 2 0.1477 Tier II
23 NC_000070.6 4 84342770 + Bnc2 NNNTCCTAAAATGTAGGACA NAG 2 0.1383 Tier II
24 NC_000077.6 11 77324329 - Ssh2 NNNTCCTAAAAGGAAGTACA NGG 2 0.125 Tier II
25 NC_000079.6 13 114152828 - Arl15 NNNTCCTAAAATGAAGGAAA NAG 2 0.1111 Tier II
26 NC_000084.6 18 75593014 - Ctif NNNTCCTTAAATGAAGGACA NAG 2 0.1111 Tier II
27 NC_000076.6 10 14428973 + Adgrg6 NNNTCCTTAAATGAAGTACA NGG 2 0.1071 Tier II
28 NC_000077.6 11 76088391 - Vps53 NNNTCCTAAAATCAAGGACC NGG 2 0.0957 Tier II
29 NC_000074.6 8 22414250 - Mrps31 NNNTCCTCAAATGCAGGACA NGG 2 0.0918 Tier II
30 NC_000071.6 5 34454910 - Fam193a NNNTCCTAAAATGCAGCACA NGG 2 0.0504 Tier II
31 NC_000083.6 17 68561449 - L3mbtl4 NNNTCCTAAAATGCTGGACA NGG 2 0.0429 Tier II
32 NC_000085.6 19 16262460 + Gnaq NNNTCCTAAAATGAAGAACA NTG 2 0.0364 Tier II
33 NC_000086.7 X 83462292 - Dmd NNNTCCTAAATTGAAGGACA NTG 2 0.012 Tier II
34 NC_000081.6 15 48364606 - Csmd3 NNNTCCTAAAATGAAGTACA NTG 2 0.0097 Tier II
35 NC_000078.6 12 89694683 - Nrxn3 NNNTCCTAAAATCAAGGACA NGC 2 0.0094 Tier II
36 NC_000078.6 12 9938616 + Gm33734 NNNCCCTAAGATGAAGGACA NGG 2 0.3595 Tier III
37 NC_000079.6 13 53761512 - Gm34439 NNNTCCAAAAATGAAGGGCA NGG 2 0.35 Tier III
38 NC_000079.6 13 9683342 + Gm36423 NNNTCTTAACATGAAGGACA NGG 2 0.3095 Tier III
39 NC_000071.6 5 28063764 - Gm35223 NNNTCCTAAGATGAAGGTCA NGG 2 0.2778 Tier III
40 NC_000076.6 10 97890529 - Gm10754 NNNTCCTAATATTAAGGACA NGG 2 0.2647 Tier III
41 NC_000068.7 2 50794782 + Gm32232 NNNACGTAAAATGAAGGACA NGG 2 0.2381 Tier III
42 NC_000075.6 9 92768654 - 4933400C23Rik NNNTCCTAAAATGAGGGACC NGG 2 0.1477 Tier III
43 NC_000068.7 2 167584247 + Gm31619 NNNTCCAAAAATGAAGGAGA NGG 2 0.1094 Tier III
44 NC_000068.7 2 167584247 + Gm11476 NNNTCCAAAAATGAAGGAGA NGG 2 0.1094 Tier III
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Other clones with same target sequence:

(none)