Construct: sgRNA BRDN0001147779

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAATGCTGGAAATTGCCCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TTK (7272)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75581

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 80010894 + TTK NNNTGCTGGAAATTGCCCTG NGG 0 1.0 Tier I
2 NC_000007.14 7 100643327 + ACTL6B NNNTGCTGGAAAGTGCCCTG NGT 2 0.0042 Tier I
3 NC_000006.12 6 76019497 + IMPG1 NNNTAATGGAAATTGCCCTG NGG 2 0.8048 Tier II
4 NC_000006.12 6 152751886 - VIP NNNTGCTGGAAATAACCCTG NGG 2 0.5826 Tier II
5 NC_000006.12 6 73824341 + CD109 NNNTACTGAAAATTGCCCTG NGG 2 0.5571 Tier II
6 NC_000011.10 11 59206936 - DTX4 NNNGGCAGGAAATTGCCCTG NGG 2 0.5469 Tier II
7 NC_000010.11 10 129522432 + MGMT NNNTGCCGGAGATTGCCCTG NGG 2 0.4469 Tier II
8 NC_000011.10 11 121121195 - TECTA NNNTACTGGACATTGCCCTG NGG 2 0.3467 Tier II
9 NC_000011.10 11 121121195 - TBCEL-TECTA NNNTACTGGACATTGCCCTG NGG 2 0.3467 Tier II
10 NC_000020.11 20 44516906 + SERINC3 NNNTGCTTGAAATTGCTCTG NGG 2 0.2917 Tier II
11 NC_000015.10 15 27363546 + GABRG3 NNNTGCTGGAGATTGCCCTC NGG 2 0.2786 Tier II
12 NC_000005.10 5 15885263 - FBXL7 NNNTGCTGGAACCTGCCCTG NGG 2 0.2078 Tier II
13 NC_000011.10 11 33663490 + KIAA1549L NNNTGCTGAATATTGCCCTG NGG 2 0.1978 Tier II
14 NC_000020.11 20 59149772 + ZNF831 NNNGGCTGGAACTTGCCCTG NGG 2 0.1645 Tier II
15 NC_000009.12 9 129146874 + PTPA NNNTGCTGGGAATTGCCCAG NGG 2 0.1587 Tier II
16 NC_000004.12 4 5139751 + STK32B NNNTGCTGCAAATTCCCCTG NGG 2 0.1469 Tier II
17 NC_000016.10 16 78302904 + WWOX NNNTGCAGGAAATTTCCCTG NGG 2 0.125 Tier II
18 NC_000005.10 5 81667149 - SSBP2 NNNTTCTGGAATTTGCCCTG NGG 2 0.1 Tier II
19 NC_000008.11 8 141399410 + PTP4A3 NNNTGCTGGGAATTGCCGTG NGG 2 0.0741 Tier II
20 NC_000012.12 12 26475748 + ITPR2 NNNTGCTAGAAATTGCCCTG NGA 2 0.0694 Tier II
21 NC_000021.9 21 40408467 + DSCAM NNNTGCTGGAAATTGCCCTA NGA 2 0.0651 Tier II
22 NC_000001.11 1 173528191 - SLC9C2 NNNTTCTGGAAATTGGCCTG NGG 2 0.0462 Tier II
23 NC_000017.11 17 52046088 - CA10 NNNTGCTGGAAATTTCCCTG NAG 2 0.037 Tier II
24 NC_000012.12 12 78060025 + NAV3 NNNTGCTGGAAATTGCTCTG NTG 2 0.0182 Tier II
25 NC_000014.9 14 74483152 - NPC2 NNNTGCTGTAAATTGCCCTG NGC 2 0.0119 Tier II
26 NC_000004.12 4 182273746 - TENM3 NNNTGCTGGAAATTGCCTTG NGT 2 0.0104 Tier II
27 NC_000001.11 1 216692687 - ESRRG NNNGGCTGGAAATTGCCCTG NGT 2 0.0101 Tier II
28 NC_000016.10 16 1543386 + IFT140 NNNTGCTGGAAAGTGCCCTG NGC 2 0.0058 Tier II
29 NC_000016.10 16 1543386 + TMEM204 NNNTGCTGGAAAGTGCCCTG NGC 2 0.0058 Tier II
30 NC_000017.11 17 9955769 - GAS7 NNNTGCTGGAACTTGCCCTG NGC 2 0.0058 Tier II
31 NC_000007.14 7 146956643 - CNTNAP2 NNNTGCTGGAAATTGTCCTG NGT 2 0.005 Tier II
32 NC_000001.11 1 236526655 + LGALS8 NNNTGCTGGAAATTGCCCTG NAA 2 0.0 Tier II
33 NC_000005.10 5 31280476 - CDH6 NNNTGCTGGCAATGGCCCTG NGG 2 0.0 Tier II
34 NC_000017.11 17 58268034 - LPO NNNTGCTTGAAATGGCCCTG NGG 2 0.0 Tier II
35 NC_000008.11 8 24535555 + ADAM7-AS1 NNNCCCTGGAAATTGCCCTG NGG 2 0.5084 Tier III
36 NC_000006.12 6 6886956 - LINC03055 NNNTGCTGGACGTTGCCCTG NGG 2 0.2889 Tier III
37 NC_000006.12 6 6886956 - LOC105374903 NNNTGCTGGACGTTGCCCTG NGG 2 0.2889 Tier III
38 NC_000008.11 8 132492205 - LOC105375765 NNNTCCTGGATATTGCCCTG NGG 2 0.2418 Tier III
39 NC_000005.10 5 15885263 - LOC107986343 NNNTGCTGGAACCTGCCCTG NGG 2 0.2078 Tier III
40 NC_000002.12 2 8104108 - LINC00299 NNNTGCTGGAAATTGCCCAT NGG 2 0.2 Tier III
41 NC_000011.10 11 33663490 + LOC105376617 NNNTGCTGAATATTGCCCTG NGG 2 0.1978 Tier III
42 NC_000013.11 13 45690635 - LINC01055 NNNTGCTGTAAATTGCCCTG NAG 2 0.1383 Tier III
43 NC_000002.12 2 239778717 + LOC150935 NNNTGCTGGAAATCGCCCTC NGG 2 0.1224 Tier III
44 NC_000020.11 20 23337278 - NXT1-AS1 NNNTGCTGGAAAGTGCTCTG NGG 2 0.1217 Tier III
45 NC_000010.11 10 97420029 + LOC644215 NNNTCCTGGAAATTTCCCTG NGG 2 0.1122 Tier III
46 NC_000014.9 14 74483152 - RAP1AP NNNTGCTGTAAATTGCCCTG NGC 2 0.0119 Tier III
47 NC_000006.12 6 125639522 - HEY2-AS1 NNNTGCTGGAAATTGCCCTC NGC 2 0.0095 Tier III
48 NC_000010.11 10 3582652 - LOC105376360 NNNTGCTGGAAATTGCCGTG NGT 2 0.0022 Tier III
49 NC_000002.12 2 127431978 + LOC105373608 NNNTCCTGGAAATGGCCCTG NGG 2 0.0 Tier III
50 NC_000013.11 13 30163746 - LINC00385 NNNTGCTGGAATTGGCCCTG NGG 2 0.0 Tier III
51 NC_000010.11 10 120930615 + LOC105378519 NNNTGCTGGAGATGGCCCTG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 39476501 + Rims2 NNNTGCTGGAAATTACCCTT NGG 2 0.6588 Tier I
2 NC_000067.6 1 43956507 - Tpp2 NNNTTCTGGAAAATGCCCTG NGG 2 0.2077 Tier I
3 NC_000073.6 7 41043395 - Gm4884 NNNTGCTGGAATTTGGCCTG NGG 2 0.0513 Tier I
4 NC_000073.6 7 41288661 - Gm5592 NNNTGCTGGAATTTGGCCTG NGG 2 0.0513 Tier I
5 NC_000074.6 8 83001471 - Rnf150 NNNTGCTGGAAATTGACCTT NGG 2 0.7 Tier II
6 NC_000080.6 14 54744689 - Slc7a8 NNNTGCTGATAATTGCCCTG NGG 2 0.5672 Tier II
7 NC_000077.6 11 57019994 - Gria1 NNNTCCTGGAAATTGCCCTT NGG 2 0.55 Tier II
8 NC_000068.7 2 20135481 + Etl4 NNNTGCTGGAAATTGAACTG NGG 2 0.4667 Tier II
9 NC_000069.6 3 95389250 - 4930558C23Rik NNNTGCTGGAAATTGAACTG NGG 2 0.4667 Tier II
10 NC_000070.6 4 58060364 + Svep1 NNNTGCTGGAAATTGAACTG NGG 2 0.4667 Tier II
11 NC_000071.6 5 108417562 - Pde6b NNNTGCTGGAAATTGAACTG NGG 2 0.4667 Tier II
12 NC_000072.6 6 112629492 - Rad18 NNNTGCTGGAAATTGAACTG NGG 2 0.4667 Tier II
13 NC_000073.6 7 67646393 - Ttc23 NNNTGCTGGAAATTGAACTG NGG 2 0.4667 Tier II
14 NC_000073.6 7 102217493 - Pgap2 NNNTGCTGGAAATTGAACTG NGG 2 0.4667 Tier II
15 NC_000074.6 8 85777016 + Itfg1 NNNTGCTGGAAATTGAACTG NGG 2 0.4667 Tier II
16 NC_000075.6 9 8087755 + Cep126 NNNTGCTGGAAATTGAACTG NGG 2 0.4667 Tier II
17 NC_000075.6 9 10880470 + Cntn5 NNNTGCTGGAAATTGAACTG NGG 2 0.4667 Tier II
18 NC_000076.6 10 127364492 - Inhbc NNNTGCTGGAAATTGAACTG NGG 2 0.4667 Tier II
19 NC_000077.6 11 72358159 - Tekt1 NNNTGCTGGAAATTGAACTG NGG 2 0.4667 Tier II
20 NC_000078.6 12 87348715 + Sptlc2 NNNTGCTGGAAATTGAACTG NGG 2 0.4667 Tier II
21 NC_000078.6 12 116525437 - Ptprn2 NNNTGCTGGAAATTGAACTG NGG 2 0.4667 Tier II
22 NC_000068.7 2 62682369 + Gca NNNTGCTGGAAATTGACCTC NGG 2 0.4286 Tier II
23 NC_000072.6 6 141821793 - Slco1a4 NNNTGCTGGAAATTGACCTC NGG 2 0.4286 Tier II
24 NC_000075.6 9 24565208 + Dpy19l2 NNNTGCTGGAAATTGACCTC NGG 2 0.4286 Tier II
25 NC_000076.6 10 77755451 - Tspear NNNTGCTGGAAATTGACCTC NGG 2 0.4286 Tier II
26 NC_000076.6 10 77764396 - Tspear NNNTGCTGGAAATTGACCTC NGG 2 0.4286 Tier II
27 NC_000077.6 11 94587792 - Acsf2 NNNTGCTGGAAATTGACCTC NGG 2 0.4286 Tier II
28 NC_000086.7 X 74256533 + Emd NNNTGCTGGAAATTGACCTC NGG 2 0.4286 Tier II
29 NC_000077.6 11 63091084 - Tekt3 NNNTCCTGTAAATTGCCCTG NGG 2 0.419 Tier II
30 NC_000076.6 10 6993026 + Oprm1 NNNTGATGGAAATTGCCCTC NGG 2 0.398 Tier II
31 NC_000076.6 10 6993026 + Ipcef1 NNNTGATGGAAATTGCCCTC NGG 2 0.398 Tier II
32 NC_000074.6 8 71500552 - Plvap NNNTGCTGGGAATTGCCCTT NGG 2 0.3889 Tier II
33 NC_000068.7 2 162973399 + L3mbtl1 NNNTCCTGGAAATTGCCCTC NGG 2 0.3367 Tier II
34 NC_000067.6 1 33657436 - Prim2 NNNTGCTGGCAATTGACCTG NGG 2 0.3333 Tier II
35 NC_000072.6 6 71714726 + Reep1 NNNTGCTGGCAATTGACCTG NGG 2 0.3333 Tier II
36 NC_000080.6 14 121992291 + Ubac2 NNNTGCTCCAAATTGCCCTG NGG 2 0.3314 Tier II
37 NC_000068.7 2 158676716 - Ppp1r16b NNNTGCTGGATATTGACCTG NGG 2 0.3077 Tier II
38 NC_000068.7 2 125689567 - Shc4 NNNTTCTAGAAATTGCCCTG NGG 2 0.3 Tier II
39 NC_000082.6 16 64527088 - Csnka2ip NNNTCCTGGAATTTGCCCTG NGG 2 0.2619 Tier II
40 NC_000072.6 6 89507645 - Chchd6 NNNTCCTGGAACTTGCCCTG NGG 2 0.2068 Tier II
41 NC_000077.6 11 116407461 + Rnf157 NNNTCCTGGAAATTGCCCTG NAG 2 0.2037 Tier II
42 NC_000072.6 6 122463611 - Rimklb NNNTGCTGGAAATTGCACTC NGG 2 0.2 Tier II
43 NC_000084.6 18 11887551 + Cables1 NNNCTCTGGAAATTGCCCTG NGG 2 0.1941 Tier II
44 NC_000070.6 4 70241960 - Cdk5rap2 NNNTGCTGGACATTGCTCTG NGG 2 0.1867 Tier II
45 NC_000073.6 7 137005493 + Mgmt NNNTGCTGGAACTTGCACTG NGG 2 0.1228 Tier II
46 NC_000085.6 19 7075160 + Macrod1 NNNTGCTGGAACTTGCTCTG NGG 2 0.1228 Tier II
47 NC_000070.6 4 129198544 - Yars NNNTGCTGGAAATTGCTCTG NAG 2 0.121 Tier II
48 NC_000070.6 4 44070889 - Gne NNNTGCTGGAACTTGCCCTC NGG 2 0.1128 Tier II
49 NC_000072.6 6 101333370 + Pdzrn3 NNNTTCTGGAAATTGCCCAG NGG 2 0.0857 Tier II
50 NC_000086.7 X 20862183 + Syn1 NNNTGCTGCAAATTGGCCTG NGG 2 0.0828 Tier II
51 NC_000070.6 4 32914725 - Ankrd6 NNNTTCTGGAACTTGCCCTG NGG 2 0.0789 Tier II
52 NC_000069.6 3 153609688 - St6galnac3 NNNTGCTGGAACGTGCCCTG NGG 2 0.0686 Tier II
53 NC_000074.6 8 13198440 + Grtp1 NNNTGCTGGAAATTGGCCTC NGG 2 0.0659 Tier II
54 NC_000070.6 4 100145758 + Ror1 NNNTGCTGGAAATTGAGCTG NGG 2 0.0588 Tier II
55 NC_000078.6 12 31606794 - Bcap29 NNNTGCTGGAAATTGAGCTG NGG 2 0.0588 Tier II
56 NC_000082.6 16 7274554 - Rbfox1 NNNTGCTGGAAATTGCCATG NGA 2 0.0374 Tier II
57 NC_000071.6 5 53114864 - Sel1l3 NNNTGCTGGAAATTGCCTTG NTG 2 0.025 Tier II
58 NC_000086.7 X 140039656 + Nup62cl NNNTGCTGGAAATTGCCCTA NGC 2 0.0208 Tier II
59 NC_000076.6 10 86807765 + Nt5dc3 NNNTGCTGGAAACTGCCCTG NGC 2 0.0175 Tier II
60 NC_000070.6 4 149531634 - Ctnnbip1 NNNTGCTGGAAATTGACCTG NGT 2 0.0161 Tier II
61 NC_000070.6 4 66175875 - Astn2 NNNAGCTGGAAATGGCCCTG NGG 2 0.0 Tier II
62 NC_000070.6 4 58060364 + Gm35402 NNNTGCTGGAAATTGAACTG NGG 2 0.4667 Tier III
63 NC_000077.6 11 63091084 - Gm36926 NNNTCCTGTAAATTGCCCTG NGG 2 0.419 Tier III
64 NC_000081.6 15 5265095 - Gm41257 NNNTGCTGCAAGTTGCCCTG NGG 2 0.3889 Tier III
65 NC_000081.6 15 38310307 + Gm35248 NNNTGCTGGGAATTGCACTG NGG 2 0.2593 Tier III
66 NC_000068.7 2 122573843 - Gm34325 NNNTGATGGAAATTCCCCTG NGG 2 0.2532 Tier III
67 NC_000076.6 10 99475808 - Gm34983 NNNTGCTGGAAATTGGACTG NGG 2 0.0718 Tier III
68 NC_000070.6 4 100145758 + Gm12701 NNNTGCTGGAAATTGAGCTG NGG 2 0.0588 Tier III
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Other clones with same target sequence:

(none)